CALS7_ARATH
ID CALS7_ARATH Reviewed; 1958 AA.
AC Q9SHJ3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 3.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Callose synthase 7;
DE EC=2.4.1.34;
DE AltName: Full=1,3-beta-glucan synthase;
DE AltName: Full=Protein GLUCAN SYNTHASE-LIKE 7;
GN Name=CALS7; Synonyms=GSL7; OrderedLocusNames=At1g06490; ORFNames=F12K11.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11283334; DOI=10.2307/3871338;
RA Hong Z., Delauney A.J., Verma D.P.S.;
RT "A cell plate-specific callose synthase and its interaction with
RT phragmoplastin.";
RL Plant Cell 13:755-768(2001).
RN [4]
RP NOMENCLATURE.
RX PubMed=16021399; DOI=10.1007/s11103-005-4526-7;
RA Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.;
RT "Two callose synthases, GSL1 and GSL5, play an essential and redundant role
RT in plant and pollen development and in fertility.";
RL Plant Mol. Biol. 58:333-349(2005).
CC -!- FUNCTION: Involved in callose synthesis at the forming cell plate
CC during cytokinesis. During plant growth and development, callose is
CC found as a transitory component of the cell plate in dividing cells, is
CC a major component of pollen mother cell walls and pollen tubes, and is
CC found as a structural component of plasmodesmatal canals (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC beta-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:21476, Rhea:RHEA-
CC COMP:11146, Rhea:RHEA-COMP:14303, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:37671, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.34;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 48 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF24822.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007592; AAF24822.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27993.1; -; Genomic_DNA.
DR PIR; F86200; F86200.
DR RefSeq; NP_172136.2; NM_100528.3.
DR AlphaFoldDB; Q9SHJ3; -.
DR SMR; Q9SHJ3; -.
DR STRING; 3702.AT1G06490.1; -.
DR CAZy; GT48; Glycosyltransferase Family 48.
DR PaxDb; Q9SHJ3; -.
DR PRIDE; Q9SHJ3; -.
DR EnsemblPlants; AT1G06490.1; AT1G06490.1; AT1G06490.
DR GeneID; 837160; -.
DR Gramene; AT1G06490.1; AT1G06490.1; AT1G06490.
DR KEGG; ath:AT1G06490; -.
DR Araport; AT1G06490; -.
DR TAIR; locus:2009185; AT1G06490.
DR eggNOG; KOG0916; Eukaryota.
DR HOGENOM; CLU_000742_0_0_1; -.
DR InParanoid; Q9SHJ3; -.
DR OrthoDB; 48442at2759; -.
DR BioCyc; ARA:AT1G06490-MON; -.
DR BioCyc; MetaCyc:AT1G06490-MON; -.
DR PRO; PR:Q9SHJ3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHJ3; baseline and differential.
DR Genevisible; Q9SHJ3; AT.
DR GO; GO:0000148; C:1,3-beta-D-glucan synthase complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0003843; F:1,3-beta-D-glucan synthase activity; IMP:TAIR.
DR GO; GO:0046527; F:glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006075; P:(1->3)-beta-D-glucan biosynthetic process; IEA:InterPro.
DR GO; GO:0080165; P:callose deposition in phloem sieve plate; IMP:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010233; P:phloem transport; IMP:TAIR.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.270; -; 1.
DR InterPro; IPR026899; FKS1-like_dom1.
DR InterPro; IPR003440; Glyco_trans_48.
DR InterPro; IPR039431; Vta1/CALS_N.
DR InterPro; IPR023175; Vta1/CALS_N_sf.
DR Pfam; PF14288; FKS1_dom1; 1.
DR Pfam; PF02364; Glucan_synthase; 1.
DR Pfam; PF04652; Vta1; 1.
DR SMART; SM01205; FKS1_dom1; 1.
PE 3: Inferred from homology;
KW Cell membrane; Cell shape; Cell wall biogenesis/degradation;
KW Glycosyltransferase; Membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..1958
FT /note="Callose synthase 7"
FT /id="PRO_0000334579"
FT TOPO_DOM 1..504
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 526..535
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..569
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 570..590
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 591..620
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 621..641
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 642..673
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 674..694
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 695..730
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 731..751
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 752..1496
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1497..1517
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1518..1547
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1548..1568
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1569..1576
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1577..1597
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1598..1640
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1641..1661
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1662..1667
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1668..1688
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1689..1742
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1743..1763
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1764..1771
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1772..1792
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1793..1812
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1813..1833
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1834..1835
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1836..1856
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1857..1878
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1879..1899
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1900..1958
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1958 AA; 228032 MW; 40AC43FFC980F692 CRC64;
MASTSSGGRG EDGRPPQMQP VRSMSRKMTR AGTMMIEHPN EDERPIDSEL VPSSLASIAP
ILRVANDIDQ DNARVAYLCR FHAFEKAHRM DPTSSGRGVR QFKTYLLHKL EEEEEITEHM
LAKSDPREIQ LYYQTFYENN IQDGEGKKTP EEMAKLYQIA TVLYDVLKTV VPQARIDDKT
LRYAKEVERK KEQYEHYNIL PLYALGAKTA VMELPEIKAA ILAVCNVDNL PRPRFHSASA
NLDEVDRERG RSFNDILEWL ALVFGFQRGN VANQREHLIL LLANIDVRKR DLENYVEIKP
STVRKLMEKY FKNYNSWCKY LRCDSYLRFP AGCDKQQLSL LYIGLYLLIW GEASNVRFMP
ECLCYIFHNM ANEVHGILFG NVYPVTGDTY EAGAPDEEAF LRNVITPIYQ VLRKEVRRNK
NGKASHSKWR NYDDLNEYFW DKRCFRLKWP MNFKADFFIH TDEISQVPNQ RHDQVSHGKR
KPKTNFVEAR TFWNLYRSFD RMWMFLVLSL QTMIIVAWHP SGSILAIFTE DVFRNVLTIF
ITSAFLNLLQ ATLDLVLSFG AWKSLKFSQI MRYITKFLMA AMWAIMLPIT YSKSVQNPTG
LIKFFSSWVG SWLHRSLYDY AIALYVLPNI LAAVFFLLPP LRRIMERSNM RIVTLIMWWA
QPKLYIGRGM HEEMFALFKY TFFWVMLLLS KLAFSYYVEI LPLVNPTKLI WDMHVVNYEW
HEFFPNATHN IGVIIAIWGP IVLVYFMDTQ IWYAIFSTLF GGIYGAFSHL GEIRTLGMLR
SRFKVVPSAF CSKLTPLPLG HAKRKHLDET VDEKDIARFS QMWNKFIHTM RDEDLISDRE
RDLLLVPSSS GDVTVVQWPP FLLASKIPIA LDMAKDFKGK EDVDLFKKIK SEYYMHYAVV
EAYETVRDII YGLLQDESDK RIVREICYEV DISIQQHRFL SEFRMTGMPL LSDKLEKFLK
ILLSDYEEDD YKSQIINVLQ DIIEIITQDV MVNGHEILER AHLQSGDIES DKKEQRFEKI
DLSLTQNISW REKVVRLLLL LTVKESAINI PQSLEARRRM TFFANSLFMN MPDAPRVRDM
LSFSVLTPYY KEDVLYSEEE LNKENEDGIT ILFYLQRIYP EEWSNYCERV NDLKRNLSEK
DKAEQLRQWV SYRGQTLSRT VRGMMYYRVA LELQCFQEYT EENATNGGYL PSESNEDDRK
AFSDRARALA DLKFTYVVSC QVYGNQKKSS ESRDRSCYNN ILQLMLKYPS LRVAYIDERE
ETVNGKSQKV FYSVLLKGCD KLDEEIYRIK LPGPPTEIGE GKPENQNHAI IFTRGEALQT
IDMNQDNYFE ECFKMRNVLQ EFDEGRRGKR NPTILGLREH IFTGSVSSLA WFMSNQETSF
VTIGQRVLAN PLRVRFHYGH PDIFDRIFHI TRGGISKASK IINLSEDIFA GYNSTLRGGY
VTHHEYIQAG KGRDVGMNQI SFFEAKVANG NGEQTLSRDV YRLGRRFDFY RMLSFYFTTV
GFYFSSMITV LTVYVFLYGR LYLVLSGLEK NILQSASVHE SNALEQALAA QSVFQLGFLM
VLPMVMEIGL EKGFRTALGD FIIMQLQLAS VFFTFQLGTK AHYFGRTILH GGSKYRATGR
GFVVFHAKFA ENYRLYSRSH FVKGLELVIL LVVYQVYGTS YRSSSTYMYI TFSMWFLVTS
WLFAPFIFNP SGFEWQKTVD DWTDWKRWMG NRGGIGIVLD KSWESWWDIE QEHLKHTNLR
GRVLEILLAL RFLLYQYGIV YHLNIARRHT TFLVYGLSWA ILLSVLLVLK MVSMGRRKFG
TDFQVMFRIL KALLFLGFLS VMTVLFVVCG LTISDLFASI LAFLPTGWAI LLIGQALRSV
FKGLGFWDSV KELGRAYEYI MGLVIFTPIA VLSWFPFVSE FQTRLLFNQA FSRGLQISMI
LAGKKDKETP STKYLGHTEE SFGLEHDTNT FNHYYLWT
