ID   VRAB_STAES              Reviewed;         382 AA.
AC   Q8CTR0;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Putative acetyl-CoA C-acetyltransferase VraB;
DE            EC=2.3.1.-;
GN   Name=vraB; OrderedLocusNames=SE_0346;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   EMBL; AE015929; AAO03943.1; -; Genomic_DNA.
DR   RefSeq; NP_763901.1; NC_004461.1.
DR   RefSeq; WP_001832124.1; NZ_WBME01000045.1.
DR   AlphaFoldDB; Q8CTR0; -.
DR   SMR; Q8CTR0; -.
DR   STRING; 176280.SE_0346; -.
DR   EnsemblBacteria; AAO03943; AAO03943; SE_0346.
DR   GeneID; 50019492; -.
DR   KEGG; sep:SE_0346; -.
DR   PATRIC; fig|176280.10.peg.319; -.
DR   eggNOG; COG0183; Bacteria.
DR   HOGENOM; CLU_031026_2_1_9; -.
DR   OMA; GHPWGAS; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR   PROSITE; PS00737; THIOLASE_2; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..382
FT                   /note="Putative acetyl-CoA C-acetyltransferase VraB"
FT                   /id="PRO_0000206432"
FT   ACT_SITE        86
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   382 AA;  41902 MW;  C3FC7540CB7A94F3 CRC64;
     MKQPVIIAAK RIAFGKYGGR LRHLEPESLL EPLFNHFTDQ YPKVMSLLDD VILGNTVGNG
     GNLARKSLLE AGLDFKIPGI TIDRQCGSGL EAVIQACRMV QSGAGTIYIA GGVESTSRAP
     WKIKRPQSVY ESEFPQFFER APFAREGEDP SMIEAAENVA KKYHISRNEQ DDFAYRSHQL
     ASKNMNNGNI SQEILPFKVK GEYFNQDESI KPQLTLRTLG RLKPLLNEGT VTVGNSCKKN
     DGAVLLIVME ENRARQLGFT EGIKFVNSAT VGVQPQYLGV GPVPAVNQLL AQERLTINDI
     NAVELNEAFS SQVIASQQQL NIPLNKLNCW GGAIATGHPY GASGAALVTR LFYMKHQFRT
     IATMGIGGGI GNAALFERWY GN