CALS9_ARATH
ID CALS9_ARATH Reviewed; 1890 AA.
AC Q9SFU6; F4JDA3; Q8LF10;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Callose synthase 9;
DE EC=2.4.1.34;
DE AltName: Full=1,3-beta-glucan synthase;
DE AltName: Full=Protein GLUCAN SYNTHASE-LIKE 10;
GN Name=CALS9; Synonyms=GSL10; OrderedLocusNames=At3g07160; ORFNames=T1B9.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1532-1890.
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11283334; DOI=10.2307/3871338;
RA Hong Z., Delauney A.J., Verma D.P.S.;
RT "A cell plate-specific callose synthase and its interaction with
RT phragmoplastin.";
RL Plant Cell 13:755-768(2001).
RN [5]
RP NOMENCLATURE.
RX PubMed=16021399; DOI=10.1007/s11103-005-4526-7;
RA Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.;
RT "Two callose synthases, GSL1 and GSL5, play an essential and redundant role
RT in plant and pollen development and in fertility.";
RL Plant Mol. Biol. 58:333-349(2005).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=18315544; DOI=10.1111/j.1365-313x.2008.03462.x;
RA Toeller A., Brownfield L., Neu C., Twell D., Schulze-Lefert P.;
RT "Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in
RT male gametophyte development and plant growth.";
RL Plant J. 54:911-923(2008).
CC -!- FUNCTION: Involved in sporophytic and gametophytic development.
CC Required for normal plant development. During pollen formation,
CC required for the entry of microspores into mitosis and microspore
CC symmetric division. May be required for correct temporal and spatial
CC control of callose deposition during pollen mitosis. During plant
CC growth and development, callose is found as a transitory component of
CC the cell plate in dividing cells, is a major component of pollen mother
CC cell walls and pollen tubes, and is found as a structural component of
CC plasmodesmatal canals. {ECO:0000269|PubMed:18315544}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC beta-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:21476, Rhea:RHEA-
CC COMP:11146, Rhea:RHEA-COMP:14303, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:37671, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.34;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout pollen development with a
CC peak at bicellular pollen stage. {ECO:0000269|PubMed:18315544}.
CC -!- DISRUPTION PHENOTYPE: Plants develop deformed or collapsed and inviable
CC pollen grains. {ECO:0000269|PubMed:18315544}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 48 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF20230.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM61660.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC012395; AAF20230.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74506.1; -; Genomic_DNA.
DR EMBL; AY085106; AAM61660.1; ALT_INIT; mRNA.
DR RefSeq; NP_187372.5; NM_111596.6.
DR AlphaFoldDB; Q9SFU6; -.
DR BioGRID; 5238; 4.
DR STRING; 3702.AT3G07160.1; -.
DR CAZy; GT48; Glycosyltransferase Family 48.
DR iPTMnet; Q9SFU6; -.
DR SwissPalm; Q9SFU6; -.
DR PaxDb; Q9SFU6; -.
DR PRIDE; Q9SFU6; -.
DR EnsemblPlants; AT3G07160.1; AT3G07160.1; AT3G07160.
DR GeneID; 819903; -.
DR Gramene; AT3G07160.1; AT3G07160.1; AT3G07160.
DR KEGG; ath:AT3G07160; -.
DR Araport; AT3G07160; -.
DR TAIR; locus:2098565; AT3G07160.
DR eggNOG; KOG0916; Eukaryota.
DR HOGENOM; CLU_000742_1_1_1; -.
DR InParanoid; Q9SFU6; -.
DR OMA; NEMARMY; -.
DR PRO; PR:Q9SFU6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SFU6; baseline and differential.
DR Genevisible; Q9SFU6; AT.
DR GO; GO:0000148; C:1,3-beta-D-glucan synthase complex; IEA:InterPro.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0003843; F:1,3-beta-D-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0046527; F:glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006075; P:(1->3)-beta-D-glucan biosynthetic process; IEA:InterPro.
DR GO; GO:0052543; P:callose deposition in cell wall; IMP:TAIR.
DR GO; GO:0048589; P:developmental growth; IMP:TAIR.
DR GO; GO:0055047; P:generative cell mitosis; IMP:TAIR.
DR GO; GO:0009556; P:microsporogenesis; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:TAIR.
DR Gene3D; 1.25.40.270; -; 1.
DR InterPro; IPR026899; FKS1-like_dom1.
DR InterPro; IPR003440; Glyco_trans_48.
DR InterPro; IPR023175; Vta1/CALS_N_sf.
DR Pfam; PF14288; FKS1_dom1; 1.
DR Pfam; PF02364; Glucan_synthase; 1.
DR SMART; SM01205; FKS1_dom1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cell shape; Cell wall biogenesis/degradation;
KW Glycosyltransferase; Membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..1890
FT /note="Callose synthase 9"
FT /id="PRO_0000334581"
FT TOPO_DOM 1..489
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..523
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..560
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 582..591
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 592..612
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 613..658
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 659..679
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 680..722
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 723..743
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 744..1457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1458..1478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1479..1512
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1513..1533
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1534..1539
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1540..1560
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1561..1609
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1610..1630
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1631..1651
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1652..1703
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1704..1724
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1725..1732
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1733..1753
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1754..1768
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1769..1789
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1790..1795
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1796..1816
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1817..1838
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1839..1859
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1860..1890
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 1577
FT /note="R -> M (in Ref. 3; AAM61660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1890 AA; 217087 MW; FA18F086C071360B CRC64;
MSRAESSWER LVNAALRRDR TGGVAGGNQS SIVGYVPSSL SNNRDIDAIL RAADEIQDED
PNIARILCEH GYSLAQNLDP NSEGRGVLQF KTGLMSVIKQ KLAKREVGTI DRSQDILRLQ
EFYRLYREKN NVDTLKEEEK QLRESGAFTD ELERKTVKRK RVFATLKVLG SVLEQLAKEI
PEELKHVIDS DAAMSEDTIA YNIIPLDAPV TTNATTTFPE VQAAVAALKY FPGLPKLPPD
FPIPATRTAD MLDFLHYIFG FQKDSVSNQR EHIVLLLANE QSRLNIPEET EPKLDDAAVR
KVFLKSLENY IKWCDYLCIQ PAWSNLEAIN GDKKLLFLSL YFLIWGEAAN IRFLPECLCY
IFHHMVREMD EILRQQVARP AESCMPVDSR GSDDGVSFLD HVIAPLYGVV SAEAFNNDNG
RAPHSAWRNY DDFNEYFWSL HSFELGWPWR TSSSFFQKPI PRKKLKTGRA KHRGKTSFVE
HRTFLHLYHS FHRLWIFLAM MFQALAIIAF NKDDLTSRKT LLQILSLGPT FVVMKFSESV
LEVIMMYGAY STTRRLAVSR IFLRFIWFGL ASVFISFLYV KSLKAPNSDS PIVQLYLIVI
AIYGGVQFFF SILMRIPTCH NIANKCDRWP VIRFFKWMRQ ERHYVGRGMY ERTSDFIKYL
LFWLVVLSAK FSFAYFLQIK PLVGPTRMIV KQNNIPYSWH DFVSRKNYNA LTVASLWAPV
VAIYLLDIHI FYTIFSAFLG FLLGARDRLG EIRSLEAIHK LFEEFPGAFM RALHVPLTNR
TSDTSHQTVD KKNKVDAAHF APFWNQIIKS LREEDYITDF EMELLLMPKN SGRLELVQWP
LFLLSSKILL AKEIAAESNS QEEILERIER DDYMKYAVEE VYHTLKLVLT ETLEAEGRLW
VERIYEDIQT SLKERNIHHD FQLNKLSLVI TRVTALLGIL KENETPEHAK GAIKALQDLY
DVMRLDILTF NMRGHYETWN LLTQAWNEGR LFTKLKWPKD PELKALVKRL YSLFTIKDSA
AHVPRNLEAR RRLQFFTNSL FMDVPPPKSV RKMLSFSVFT PYYSEVVLYS MAELTKRNED
GISILFYLQK IYPDEWKNFL ARIGRDENAL EGDLDNERDI LELRFWASYR GQTLARTVRG
MMYYRKALML QSYLERKAGN DATDAEGFEL SPEARAQADL KFTYVVTCQI YGRQKEDQKP
EAVDIALLMQ RNEALRIAYI DVVDSPKEGK SHTEYYSKLV KADISGKDKE IYSIKLPGDP
KLGEGKPENQ NHAIVFTRGN AIQTIDMNQD NYFEEALKMR NLLEEFDRDH GIRPPTILGV
REHVFTGSVS SLASFMSNQE TSFVTLGQRV LAKPLKIRMH YGHPDVFDRV FHITRGGISK
ASRVINISED IFAGFNTTLR QGNVTHHEYI QVGKGRDVGL NQIALFEGKV AGGNGEQVLS
RDVYRLGQLL DFFRMMSFFF TTVGFYLCTM LTVLTVYIFL YGRAYLALSG VGATIRERAI
LLDDTALSAA LNAQFLFQIG VFTAVPMVLG FILEQGFLQA IVSFITMQFQ LCTVFFTFSL
GTRTHYFGRT ILHGGARYQA TGRGFVVKHI KFSENYRLYS RSHFVKAMEV ILLLVVYLAY
GNDEAGAVSY ILLTVSSWFL AVSWLFAPYL FNPAGFEWQK VVEDFKEWTN WLFYRGGIGV
KGAESWEAWW EEELSHIRTL SGRIMETILS LRFFIFQYGI VYKLKLQGSD TSFAVYGWSW
VAFAMIIVLF KVFTFSQKIS VNFQLLLRFI QGLSLLMALA GIIVAVVLTP LSVTDIFACV
LAFIPTGWGI LSIACAWKPV LKRMGMWKSI RSLARLYDAL MGMLIFLPVA LCSWFPFVST
FQTRMMFNQA FSRGLEISLI LAGDNPNSGL
