VRDA_ASPPA
ID VRDA_ASPPA Reviewed; 343 AA.
AC B9WYE6;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Versiconal hemiacetal acetate reductase;
DE EC=1.1.1.353;
DE AltName: Full=VHA reductase;
GN Name=vrdA;
OS Aspergillus parasiticus.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=5067;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 3-27 AND
RP 214-25, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 26691 / NRRL 2999 / CBS 921.70;
RX PubMed=19211038; DOI=10.1016/j.fgb.2008.12.005;
RA Shima Y., Shiina M., Shinozawa T., Ito Y., Nakajima H., Adachi Y., Yabe K.;
RT "Participation in aflatoxin biosynthesis by a reductase enzyme encoded by
RT vrdA gene outside the aflatoxin gene cluster.";
RL Fungal Genet. Biol. 46:221-231(2009).
RN [2]
RP IDENTIFICATION.
RX PubMed=16349333; DOI=10.1128/aem.60.7.2561-2567.1994;
RA Matsushima K., Ando Y., Hamasaki T., Yabe K.;
RT "Purification and characterization of two versiconal hemiacetal acetate
RT reductases involved in aflatoxin biosynthesis.";
RL Appl. Environ. Microbiol. 60:2561-2567(1994).
CC -!- FUNCTION: Catalyzes 3 reactions: from hydroxyversicolorone (HVN) to
CC versicolorone (VONE), from versiconal hemiacetal acetate (VHA) to
CC versiconol acetate (VOAc) and from versiconal (VHOH) to versiconol
CC (VOH). Probably not an aflatoxin biosynthesis gene: may be involved in
CC the vertical branching steps connecting the main pathway from HVN to
CC VHOH with the side pathway from VONE to VOH.
CC {ECO:0000269|PubMed:19211038}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S)-versicolorone + NADP(+) = 1'-hydroxyversicolorone + H(+)
CC + NADPH; Xref=Rhea:RHEA:35691, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:73281, ChEBI:CHEBI:77907;
CC EC=1.1.1.353; Evidence={ECO:0000269|PubMed:19211038};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-versiconol acetate + NADP(+) = (2S,3S)-versiconal
CC hemiacetal acetate + H(+) + NADPH; Xref=Rhea:RHEA:35695,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:72673, ChEBI:CHEBI:77975; EC=1.1.1.353;
CC Evidence={ECO:0000269|PubMed:19211038};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-versiconol + NADP(+) = (2S-3S)-versiconal hemiacetal +
CC H(+) + NADPH; Xref=Rhea:RHEA:35699, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:77947,
CC ChEBI:CHEBI:77950; EC=1.1.1.353;
CC Evidence={ECO:0000269|PubMed:19211038};
CC -!- DISRUPTION PHENOTYPE: Aflatoxin production is not inhibited, while the
CC enzyme activity catalyzing the reaction from versiconal hemiacetal
CC acetate (VHA), to versiconol acetate (VOAc), which branches from the
CC main pathway, significantly decreases. {ECO:0000269|PubMed:19211038}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto
CC reductase 2 subfamily. {ECO:0000305}.
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DR EMBL; AB025578; BAH24200.1; -; Genomic_DNA.
DR EMBL; AB025579; BAH24201.1; -; mRNA.
DR AlphaFoldDB; B9WYE6; -.
DR SMR; B9WYE6; -.
DR KEGG; ag:BAH24200; -.
DR BRENDA; 1.1.1.353; 523.
DR GO; GO:0102974; F:hydroxyversicolorone reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0102975; F:versiconal hemiacetal acetate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0102976; F:versiconal reductase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR SUPFAM; SSF51430; SSF51430; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; NADP; Oxidoreductase.
FT CHAIN 1..343
FT /note="Versiconal hemiacetal acetate reductase"
FT /id="PRO_0000424161"
FT ACT_SITE 59
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 144
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 229..239
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT SITE 87
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 343 AA; 38698 MW; 32AA44AEE1DEDAA3 CRC64;
MEYARLGDSG LKVSKVILGC MGYGSPEWQG WVLNEEESLP LIEHAYNKGI RTWDTADMYS
HGKSEEIVGK ALKKYNIPRS RVVILTKCYF GVDDQGNFPS PLSTGRQNEG DYLNRVGLSR
RHILEAVDAS VERLGTYIDV LQIHRLDRET PREEIMRALN DVVESGKARY IGASSMAAWE
FQTLQNIAIR NGWHKFISMQ NYHNLIAREE EREMIPYCLD SGVSLIPWSP VARGALARPW
ASRSTLRENT DAGISILVRA RESESDKAII DRVEELADKK GISMAQVAIA WSLSHPSEYP
IVGLNTKDRI DEAVASVQVK LTPEEIQYLE EPYVPKAIHP GER