VRET_DROME
ID VRET_DROME Reviewed; 691 AA.
AC Q9VCU7;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein vreteno;
GN Name=vret; ORFNames=CG4771;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP INTERACTION WITH AUB AND PIWI, AND MUTAGENESIS OF CYS-266; GLY-407;
RP 536-ILE-GLN-537; PRO-602 AND GLY-610.
RX PubMed=21831924; DOI=10.1242/dev.069187;
RA Zamparini A.L., Davis M.Y., Malone C.D., Vieira E., Zavadil J.,
RA Sachidanandam R., Hannon G.J., Lehmann R.;
RT "Vreteno, a gonad-specific protein, is essential for germline development
RT and primary piRNA biogenesis in Drosophila.";
RL Development 138:4039-4050(2011).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21863019; DOI=10.1038/emboj.2011.308;
RA Handler D., Olivieri D., Novatchkova M., Gruber F.S., Meixner K.,
RA Mechtler K., Stark A., Sachidanandam R., Brennecke J.;
RT "A systematic analysis of Drosophila TUDOR domain-containing proteins
RT identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway
RT factors.";
RL EMBO J. 30:3977-3993(2011).
CC -!- FUNCTION: Gonad-specific protein essential for germline development to
CC repress transposable elements and preventing their mobilization, which
CC is essential for the germline integrity. Acts via the piRNA metabolic
CC process in both germline and somatic gonadal tissues by mediating the
CC repression of transposable elements during meiosis. Required for
CC primary piRNA biogenesis in both germline and somatic gonadal tissues.
CC {ECO:0000269|PubMed:21831924, ECO:0000269|PubMed:21863019}.
CC -!- SUBUNIT: Interacts with aub and piwi. {ECO:0000269|PubMed:21831924}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21831924,
CC ECO:0000269|PubMed:21863019}. Note=Localizes to Yb bodies in follicle
CC cells and to the meiotic nuage, also named P granule, in germline
CC cells.
CC -!- TISSUE SPECIFICITY: Gonad-specific. {ECO:0000269|PubMed:21831924}.
CC -!- DISRUPTION PHENOTYPE: Both female and male vret mutants are sterile.
CC {ECO:0000269|PubMed:21831924}.
CC -!- MISCELLANEOUS: 'Vreteno' means 'woolspinning spindle' in Bulgarian,
CC referring to its eggshell phenotype. {ECO:0000305|PubMed:21831924}.
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DR EMBL; AE014297; AAF56058.1; -; Genomic_DNA.
DR EMBL; AY058673; AAL13902.1; -; mRNA.
DR RefSeq; NP_651092.1; NM_142835.4.
DR AlphaFoldDB; Q9VCU7; -.
DR BioGRID; 67644; 38.
DR IntAct; Q9VCU7; 13.
DR MINT; Q9VCU7; -.
DR STRING; 7227.FBpp0083716; -.
DR PaxDb; Q9VCU7; -.
DR DNASU; 42695; -.
DR EnsemblMetazoa; FBtr0084323; FBpp0083716; FBgn0263143.
DR GeneID; 42695; -.
DR KEGG; dme:Dmel_CG4771; -.
DR UCSC; CG4771-RA; d. melanogaster.
DR CTD; 42695; -.
DR FlyBase; FBgn0263143; vret.
DR VEuPathDB; VectorBase:FBgn0263143; -.
DR eggNOG; KOG2039; Eukaryota.
DR HOGENOM; CLU_026119_0_0_1; -.
DR InParanoid; Q9VCU7; -.
DR OMA; RYLNVKY; -.
DR OrthoDB; 449514at2759; -.
DR PhylomeDB; Q9VCU7; -.
DR SignaLink; Q9VCU7; -.
DR BioGRID-ORCS; 42695; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42695; -.
DR PRO; PR:Q9VCU7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0263143; Expressed in cleaving embryo and 12 other tissues.
DR Genevisible; Q9VCU7; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0043186; C:P granule; IDA:UniProtKB.
DR GO; GO:0070725; C:Yb body; IPI:FlyBase.
DR GO; GO:0007293; P:germarium-derived egg chamber formation; IMP:FlyBase.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0010526; P:negative regulation of transposition, RNA-mediated; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0030719; P:P granule organization; IBA:GO_Central.
DR GO; GO:0034587; P:piRNA metabolic process; IMP:FlyBase.
DR CDD; cd04508; TUDOR; 2.
DR InterPro; IPR002999; Tudor.
DR Pfam; PF00567; TUDOR; 2.
DR SMART; SM00333; TUDOR; 2.
DR PROSITE; PS50304; TUDOR; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Meiosis; Reference proteome; Repeat.
FT CHAIN 1..691
FT /note="Protein vreteno"
FT /id="PRO_0000425213"
FT DOMAIN 366..427
FT /note="Tudor 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00211"
FT DOMAIN 573..630
FT /note="Tudor 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00211"
FT REGION 128..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 266
FT /note="C->Y: In vret(46); egg chambers are defective with
FT abnormal nurse cell numbers and improper oocyte
FT positioning. Follicle cells often fail to encapsulate egg
FT chambers, and occasionally form disorganized multicellular
FT layers."
FT /evidence="ECO:0000269|PubMed:21831924"
FT MUTAGEN 407
FT /note="G->E: In vret(39); egg chambers are defective with
FT abnormal nurse cell numbers and improper oocyte
FT positioning. Follicle cells often fail to encapsulate egg
FT chambers, and occasionally form disorganized multicellular
FT layers."
FT /evidence="ECO:0000269|PubMed:21831924"
FT MUTAGEN 536..537
FT /note="IQ->LK: In vret(48-60); the germarium is filled with
FT germ cells that maintain a round spectrosome, suggesting
FT failure of germline stem cells to differentiate."
FT /evidence="ECO:0000269|PubMed:21831924"
FT MUTAGEN 602
FT /note="P->L: In vret(49); ventralized eggs with no defects
FT in somatic gonadal cell patterning, germ cell
FT differentiation or oocyte specification."
FT /evidence="ECO:0000269|PubMed:21831924"
FT MUTAGEN 610
FT /note="G->E: In vret(48-15); ventralized eggs with no
FT defects in somatic gonadal cell patterning, germ cell
FT differentiation or oocyte specification."
FT /evidence="ECO:0000269|PubMed:21831924"
SQ SEQUENCE 691 AA; 78964 MW; EC93E72166CF50C5 CRC64;
MESESSQDDW SAFDPMSREY YDQVGNRYTN ESGQKLIMEA PLPPKDEKEQ RATRQAKYPY
LVVPKSSPYV TEKMLINFFG RALIKEMEFR RLSRVYFVYF ENIASLETAQ QRVQRYPNLI
KCITGRPQKE REITSDPVTS TEPMPTPGPA ISATERTPVN HNREVPISTG GQNHPEFFRP
PLVTKDDYKR GSLLATNDPI QRYLNVKYEF ALERHDIYKL KDETRIPQVV LHFRSGRTIP
LSTVAVTEED KTKSLLEDGV SKCVVCQNWT DTFCKLCKMP FCNASCFADV AEQHKQACGK
GEILNLDEKV GRKYPKPGLP PSGSKVRITA FEQTNVVYVR SADIQIDIAY YTVLTEVMML
GKDASKLQST PVCGQIVLYK FEGHMSRAMV LNVDNIKEIY VVFIDFGSVE VTQLERLYEC
SSYLAGLTCY PVAVKLRGVP RRFVGPNIRE VMYELDQSLV FNIKYSSREY DTSKGMQVVV
MTEIDINRSL NRLFKTILTP VEPSVSDLGY KEDCLPYIPL HCGKNINVVV MDNTFIQCGF
IYCTSIDLAY EVTKMQRDIQ EYGEKIAKCA TYAPPINELC IAKYEGKWRR GLSVELVGDG
YPSILFIDYG NIVPTHVTDI RPYPPQFIFP IMTTQLDLIG VPEKPTDEQI KWLDKNYPIG
SVITCSEITF DEETNSYSTR IEKLQEFLSL D
