VRN1_ARATH
ID VRN1_ARATH Reviewed; 341 AA.
AC Q8L3W1; Q9LJ67;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=B3 domain-containing transcription factor VRN1;
DE AltName: Full=Protein VERNALIZATION 1;
GN Name=VRN1; OrderedLocusNames=At3g18990; ORFNames=K13E13.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12114624; DOI=10.1126/science.1072147;
RA Levy Y.Y., Mesnage S., Mylne J.S., Gendall A.R., Dean C.;
RT "Multiple roles of Arabidopsis VRN1 in vernalization and flowering time
RT control.";
RL Science 297:243-246(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
CC -!- FUNCTION: Involved in the regulation of vernalization. Acts as
CC transcriptional repressor of FLC, a major target of the vernalization
CC pathway. Binds DNA in vitro in a non-sequence-specific manner.
CC {ECO:0000269|PubMed:12114624}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00326,
CC ECO:0000269|PubMed:12114624}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and at lower levels in aerial
CC parts. {ECO:0000269|PubMed:12114624}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01695.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF289051; AAM76972.1; -; Genomic_DNA.
DR EMBL; AF289052; AAM76973.1; -; mRNA.
DR EMBL; AP000735; BAB01695.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76178.1; -; Genomic_DNA.
DR EMBL; BT024731; ABD59069.1; -; mRNA.
DR EMBL; AK230388; BAF02186.1; -; mRNA.
DR EMBL; AB493620; BAH30458.1; -; mRNA.
DR RefSeq; NP_188529.2; NM_112785.5.
DR PDB; 4I1K; X-ray; 1.60 A; A/B=208-341.
DR PDBsum; 4I1K; -.
DR AlphaFoldDB; Q8L3W1; -.
DR BMRB; Q8L3W1; -.
DR SMR; Q8L3W1; -.
DR BioGRID; 6765; 4.
DR IntAct; Q8L3W1; 1.
DR STRING; 3702.AT3G18990.1; -.
DR iPTMnet; Q8L3W1; -.
DR PaxDb; Q8L3W1; -.
DR PRIDE; Q8L3W1; -.
DR ProteomicsDB; 242709; -.
DR DNASU; 821432; -.
DR EnsemblPlants; AT3G18990.1; AT3G18990.1; AT3G18990.
DR GeneID; 821432; -.
DR Gramene; AT3G18990.1; AT3G18990.1; AT3G18990.
DR KEGG; ath:AT3G18990; -.
DR Araport; AT3G18990; -.
DR TAIR; locus:2085849; AT3G18990.
DR eggNOG; ENOG502SVH3; Eukaryota.
DR HOGENOM; CLU_015069_1_1_1; -.
DR InParanoid; Q8L3W1; -.
DR PhylomeDB; Q8L3W1; -.
DR PRO; PR:Q8L3W1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8L3W1; baseline and differential.
DR Genevisible; Q8L3W1; AT.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0010048; P:vernalization response; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 2.
DR Gene3D; 2.40.330.10; -; 2.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR Pfam; PF02362; B3; 2.
DR SMART; SM01019; B3; 2.
DR SUPFAM; SSF101936; SSF101936; 2.
DR PROSITE; PS50863; B3; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..341
FT /note="B3 domain-containing transcription factor VRN1"
FT /id="PRO_0000375120"
FT DNA_BIND 5..98
FT /note="TF-B3 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT DNA_BIND 244..338
FT /note="TF-B3 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 166..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..223
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 224..236
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 243..247
FT /evidence="ECO:0007829|PDB:4I1K"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:4I1K"
FT HELIX 263..269
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 275..281
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 284..293
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 296..299
FT /evidence="ECO:0007829|PDB:4I1K"
FT HELIX 303..309
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 317..322
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 324..327
FT /evidence="ECO:0007829|PDB:4I1K"
FT STRAND 329..335
FT /evidence="ECO:0007829|PDB:4I1K"
SQ SEQUENCE 341 AA; 39275 MW; E40ABFB2C8CC3114 CRC64;
MPRPFFHKLI FSSTIQEKRL RVPDKFVSKF KDELSVAVAL TVPDGHVWRV GLRKADNKIW
FQDGWQEFVD RYSIRIGYLL IFRYEGNSAF SVYIFNLSHS EINYHSTGLM DSAHNHFKRA
RLFEDLEDED AEVIFPSSVY PSPLPESTVP ANKGYASSAI QTLFTGPVKA EEPTPTPKIP
KKRGRKKKNA DPEEINSSAP RDDDPENRSK FYESASARKR TVTAEERERA INAAKTFEPT
NPFFRVVLRP SYLYRGCIMY LPSGFAEKYL SGISGFIKVQ LAEKQWPVRC LYKAGRAKFS
QGWYEFTLEN NLGEGDVCVF ELLRTRDFVL KVTAFRVNEY V
