ID   VRPI_BPT7               Reviewed;          64 AA.
AC   P03704;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 77.
DE   RecName: Full=Bacterial RNA polymerase inhibitor;
DE   AltName: Full=Gene product 2;
DE            Short=Gp2;
GN   OrderedLocusNames=2;
OS   Escherichia phage T7 (Bacteriophage T7).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6864790; DOI=10.1016/s0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of
RT   T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7310871; DOI=10.1016/0022-2836(81)90178-9;
RA   Dunn J.J., Studier F.W.;
RT   "Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to
RT   the beginning of gene 4.";
RL   J. Mol. Biol. 148:303-330(1981).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH RPOC.
RX   PubMed=10369763; DOI=10.1006/jmbi.1999.2782;
RA   Nechaev S., Severinov K.;
RT   "Inhibition of Escherichia coli RNA polymerase by bacteriophage T7 gene 2
RT   protein.";
RL   J. Mol. Biol. 289:815-826(1999).
RN   [4]
RP   STRUCTURE BY NMR.
RX   PubMed=20133868; DOI=10.1073/pnas.0907908107;
RA   Camara B., Liu M., Reynolds J., Shadrin A., Liu B., Kwok K., Simpson P.,
RA   Weinzierl R., Severinov K., Cota E., Matthews S., Wigneshweraraj S.R.;
RT   "T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by
RT   antagonizing stable DNA strand separation near the transcription start
RT   site.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:2247-2252(2010).
RN   [5]
RP   STRUCTURE BY NMR.
RX   PubMed=22819324; DOI=10.1016/j.molcel.2012.06.013;
RA   James E., Liu M., Sheppard C., Mekler V., Camara B., Liu B., Simpson P.,
RA   Cota E., Severinov K., Matthews S., Wigneshweraraj S.;
RT   "Structural and mechanistic basis for the inhibition of Escherichia coli
RT   RNA polymerase by T7 Gp2.";
RL   Mol. Cell 47:755-766(2012).
CC   -!- FUNCTION: Acts as an inhibitor of bacterial RNA polymerase by
CC       interacting with RpoC subunit and thus preventing promoter recognition
CC       by RpoE. Non-DNA-binding transcription factor that plays a role in the
CC       transcriptional switch that abolishes transcription of the viral early
CC       genes (class I) by the host RNA polymerase but allows transcription of
CC       late genes (class II and class III) by T7 RNA polymerase.
CC       {ECO:0000269|PubMed:10369763}.
CC   -!- SUBUNIT: Interacts with host RpoC; this interaction inhibits host RNA
CC       polymerase activity. {ECO:0000269|PubMed:10369763}.
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DR   EMBL; V01146; CAA24399.1; -; Genomic_DNA.
DR   EMBL; V01127; CAA24342.1; -; Genomic_DNA.
DR   PIR; G94615; JRBP27.
DR   RefSeq; NP_041969.1; NC_001604.1.
DR   PDB; 2LMC; NMR; -; A=1-60.
DR   PDB; 2WNM; NMR; -; A=1-64.
DR   PDB; 4LK0; X-ray; 3.91 A; M/N=1-64.
DR   PDB; 4LLG; X-ray; 3.79 A; M/N=1-64.
DR   PDBsum; 2LMC; -.
DR   PDBsum; 2WNM; -.
DR   PDBsum; 4LK0; -.
DR   PDBsum; 4LLG; -.
DR   BMRB; P03704; -.
DR   SMR; P03704; -.
DR   IntAct; P03704; 1.
DR   MINT; P03704; -.
DR   GeneID; 1261073; -.
DR   KEGG; vg:1261073; -.
DR   EvolutionaryTrace; P03704; -.
DR   Proteomes; UP000000840; Genome.
DR   GO; GO:0039653; P:suppression by virus of host transcription; IDA:UniProtKB.
DR   Gene3D; 3.10.20.510; -; 1.
DR   InterPro; IPR016412; RNA_pol_inhibitor.
DR   InterPro; IPR038715; RNA_pol_inhibitor_sf.
DR   Pfam; PF16857; RNA_pol_inhib; 1.
DR   PIRSF; PIRSF004348; RNA_polymerase_inhibitor_gp2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Bacterial host gene expression shutoff by virus;
KW   Bacterial host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Reference proteome.
FT   CHAIN           1..64
FT                   /note="Bacterial RNA polymerase inhibitor"
FT                   /id="PRO_0000106481"
FT   STRAND          13..21
FT                   /evidence="ECO:0007829|PDB:2LMC"
FT   STRAND          26..31
FT                   /evidence="ECO:0007829|PDB:2LMC"
FT   HELIX           36..45
FT                   /evidence="ECO:0007829|PDB:2LMC"
FT   HELIX           48..50
FT                   /evidence="ECO:0007829|PDB:2LMC"
FT   STRAND          53..60
FT                   /evidence="ECO:0007829|PDB:2LMC"
SQ   SEQUENCE   64 AA;  7175 MW;  D42C11A4C116862F CRC64;
     MSNVNTGSLS VDNKKFWATV ESSEHSFEVP IYAETLDEAL ELAEWQYVPA GFEVTRVRPC
     VAPK