ID   VRPO_LAMBD              Reviewed;         333 AA.
AC   P03688;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Replication protein O;
GN   Name=O;
OS   Escherichia phage lambda (Bacteriophage lambda).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus.
OX   NCBI_TaxID=10710;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA   Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT   "Nucleotide sequence of bacteriophage lambda DNA.";
RL   J. Mol. Biol. 162:729-773(1982).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=704348; DOI=10.1093/nar/5.9.3141;
RA   Scherer G.;
RT   "Nucleotide sequence of the O gene and of the origin of replication in
RT   bacteriophage lambda DNA.";
RL   Nucleic Acids Res. 5:3141-3156(1978).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-162.
RX   PubMed=478301; DOI=10.1016/0378-1119(79)90060-x;
RA   Ovchinnikov Y.A., Guryev S.O., Krayev A.S., Monastyrskaya G.S.,
RA   Skryabin K.G., Sverdlov E.D., Zakharyev V.M., Bayev A.A.;
RT   "Primary structure of an EcoRI fragment of lambda imm434 DNA containing
RT   regions cI-cro of phage 434 and cII-o of phage lambda.";
RL   Gene 6:235-249(1979).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-99.
RX   PubMed=264238; DOI=10.1038/272410a0;
RA   Schwarz E., Scherer G., Hobom G., Koessel H.;
RT   "Nucleotide sequence of cro, cII and part of the O gene in phage lambda
RT   DNA.";
RL   Nature 272:410-414(1978).
CC   -!- FUNCTION: Necessary for the bidirectional replication of DNA. It
CC       interacts with the ori (origin of replication) region of the genome
CC       during the initiation of replication.
CC   -!- SIMILARITY: Belongs to the phage O protein family. {ECO:0000305}.
CC   -!- CAUTION: The amino end of replication protein O may be as shown or may
CC       begin at residue 22. There is a UGA terminator after residue 299. In
CC       vitro, this terminator codon is skipped and read-through occurs. It is
CC       not known whether read-through occurs in vivo. {ECO:0000305}.
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DR   EMBL; J02459; AAA96584.1; ALT_TERM; Genomic_DNA.
DR   EMBL; J02460; AAA32247.1; -; Genomic_DNA.
DR   PIR; F94614; ORBPL.
DR   RefSeq; NP_040631.1; NC_001416.1.
DR   IntAct; P03688; 1.
DR   GeneID; 2703490; -.
DR   KEGG; vg:3827060; -.
DR   Proteomes; UP000001711; Genome.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IDA:UniProtKB.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR006497; Phage_lambda_VrpO_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04492; Phage_rep_O; 1.
DR   TIGRFAMs; TIGR01610; phage_O_Nterm; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; Reference proteome.
FT   CHAIN           1..333
FT                   /note="Replication protein O"
FT                   /id="PRO_0000077702"
FT   REGION          117..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   333 AA;  37772 MW;  362FF405864AA208 CRC64;
     MTNTAKILNF GRGNFAGQER NVADLDDGYA RLSNMLLEAY SGADLTKRQF KVLLAILRKT
     YGWNKPMDRI TDSQLSEITK LPVKRCNEAK LELVRMNIIK QQGGMFGPNK NISEWCIPQN
     EGKSPKTRDK TSLKLGDCYP SKQGDTKDTI TKEKRKDYSS ENSGESSDQP ENDLSVVKPD
     AAIQSGSKWG TAEDLTAAEW MFDMVKTIAP SARKPNFAGW ANDIRLMRER DGRNHRDMCV
     LFRWACQDNF WSGNVLSPAK LRDKWTQLEI NRNKQQAGVT ASKPKLDLTN TDWIYGVDLK
     TSPHRWLTLT VSRCVGSPTT CRNSTTKSRR YSR