ID   VRP_PINMA               Reviewed;         238 AA.
AC   P86947;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Valine-rich protein;
DE   AltName: Full=Alveolin-like protein;
DE   Flags: Precursor;
OS   Pinctada maxima (Silver-lipped pearl oyster) (White-lipped pearl oyster).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=104660;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|PubMed:19915030};
RX   PubMed=19915030; DOI=10.1093/molbev/msp278;
RA   Jackson D.J., McDougall C., Woodcroft B., Moase P., Rose R.A., Kube M.,
RA   Reinhardt R., Rokhsar D.S., Montagnani C., Joubert C., Piquemal D.,
RA   Degnan B.M.;
RT   "Parallel evolution of nacre building gene sets in molluscs.";
RL   Mol. Biol. Evol. 27:591-608(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 35-52; 93-120; 124-130 AND 173-210, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23213212; DOI=10.1073/pnas.1210552109;
RA   Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C.,
RA   Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.;
RT   "Different secretory repertoires control the biomineralization processes of
RT   prism and nacre deposition of the pearl oyster shell.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23213212}.
CC   -!- TISSUE SPECIFICITY: Prismatic layer of shell (at protein level).
CC       Expressed primarily in the mantle with highest level in the mantle edge
CC       and lower level in the mantle pallium. {ECO:0000269|PubMed:23213212}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=GT280520; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=GT282238; Type=Miscellaneous discrepancy; Note=Premature stop codon at position 213.; Evidence={ECO:0000305};
CC       Sequence=GT282710; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=GT284449; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; GT277998; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT279116; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT280347; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT280520; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT280708; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281273; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281325; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT281479; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282238; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282452; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282632; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282646; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282710; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282729; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT283005; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT283604; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT283616; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT283704; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT283832; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284347; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284449; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; EZ420144; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P86947; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..238
FT                   /note="Valine-rich protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000413081"
FT   CONFLICT        106
FT                   /note="Q -> P (in Ref. 1; GT282452)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        113
FT                   /note="K -> R (in Ref. 1; GT282452)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        130
FT                   /note="R -> K (in Ref. 1; GT277998)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        141
FT                   /note="Q -> H (in Ref. 1; GT281479)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        191
FT                   /note="V -> C (in Ref. 1; GT280520)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        200
FT                   /note="E -> K (in Ref. 1; GT281325/GT283832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        205
FT                   /note="P -> S (in Ref. 1; GT283005/GT280708)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   238 AA;  27079 MW;  99E2D02ECD3A76CB CRC64;
     MQAVLLVVAL FGAALADPPK KIHLPTPKVK RVEKLAHQEH GVNVVQAVPI TKTVVRTVNV
     PKTLIQEVPI HVYTDLVLKS PVPRRKVIDI KKTVLQPHIR HVPVDQPYVV HRKVPVIHTK
     IVRQPRPVPR IVNIPRLKVV QKQINRIIDV PRLVTRERVH RVIKPVPVVR TRVQHVDVDV
     PMRVVVPEPV VQDRHTQSVE TVPVPHDVVR KVKVPKTFVL KDLIPVPEKA GRHYSSDK