VRTL_PENAE
ID VRTL_PENAE Reviewed; 503 AA.
AC D7PHY8;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=Efflux pump vrtL {ECO:0000303|PubMed:20534346};
DE AltName: Full=Viridicatumtoxin synthesis protein L {ECO:0000303|PubMed:20534346};
GN Name=vrtL {ECO:0000303|PubMed:20534346};
OS Penicillium aethiopicum.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=36650;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=IBT 5753;
RX PubMed=20534346; DOI=10.1016/j.chembiol.2010.03.015;
RA Chooi Y.H., Cacho R., Tang Y.;
RT "Identification of the viridicatumtoxin and griseofulvin gene clusters from
RT Penicillium aethiopicum.";
RL Chem. Biol. 17:483-494(2010).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=19168978; DOI=10.1038/ja.2008.84;
RA Zheng C.J., Yu H.E., Kim E.H., Kim W.G.;
RT "Viridicatumtoxin B, a new anti-MRSA agent from Penicillium sp. FR11.";
RL J. Antibiot. 61:633-637(2008).
RN [3]
RP BIOTECHNOLOGY.
RX PubMed=27049441; DOI=10.1038/ja.2016.35;
RA Inokoshi J., Nakamura Y., Komada S., Komatsu K., Umeyama H., Tomoda H.;
RT "Inhibition of bacterial undecaprenyl pyrophosphate synthase by small
RT fungal molecules.";
RL J. Antibiot. 69:798-805(2016).
CC -!- FUNCTION: Efflux pump; part of the gene cluster that mediates the
CC biosynthesis of viridicatumtoxin, a tetracycline-like fungal
CC meroterpenoid with a unique, fused spirobicyclic ring system
CC (PubMed:20534346). {ECO:0000269|PubMed:20534346}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- BIOTECHNOLOGY: Viridicatumtoxin and its derivative, viridicatumtoxin B,
CC exhibit anti-methicillin-resistant Staphylococcus aureus (anti-MRSA)
CC activity (PubMed:19168978). Moreover, viridicatumtoxin and a C2 acetyl
CC analog, spirohexaline, have been demonstrated to inhibit bacterial
CC undecaprenyl diphosphate synthase, a potential new target for
CC antibiotic development (PubMed:27049441). {ECO:0000269|PubMed:19168978,
CC ECO:0000269|PubMed:27049441}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; GU574477; ADI24938.1; -; Genomic_DNA.
DR AlphaFoldDB; D7PHY8; -.
DR SMR; D7PHY8; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..503
FT /note="Efflux pump vrtL"
FT /id="PRO_0000436819"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 503 AA; 55280 MW; C2C3C15044844D66 CRC64;
MSKLSDNHSS ASEGEKEAGD LESGPTAISS EPSFDDADRD PNLITWDGPK DPENPKNWPK
GLRWKNTWTV SLFVFISPVS SSMIAPAMSD LAKSLGMHAE IEIYLSLSIF ILAYSIGPIF
FGPASELYGR VRLLQISNVW YLAWNLGCGF ATTKGQLFAF RFLAGIGGSA PLAIGGGAIS
DMWTAEERGK AMGVYTLGPL LGPVVGPIAG GFIAEYSTWR WVFWSTSAAA LAVQVVGFFW
LQECHPGTLL RKRRDRLAKE TGNENLHTAE KVETLGYKLL HAFERPVKMF TTQPIVFCMA
IYMAYLFGIS YLMFATFPEI WTVVYHESPG IGGLNYLSIA IGSFIGLFFN LKLVDRIYRS
LKARNNGVGK PEYRMPSLAV GSVISTIGLF WYGWSIGNTH WIMPNIGALI FAMGTISCLQ
GMQTYIVDSY QTYAASAMAA CAVLRSLCGF GFPLFAPYMY NSLGYGWGTS LLAFITMVVG
WGAPFAFWHF GPRLRAMSKY ASG