VS41_GIAIN
ID VS41_GIAIN Reviewed; 687 AA.
AC P92127;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Variant-specific surface protein VSP4A1;
DE AltName: Full=CRISP-90;
DE Flags: Precursor;
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=O2-4A1;
RX PubMed=9178264; DOI=10.1016/s0166-6851(97)90002-5;
RA Papanastasiou P., Bruderer T., Li Y., Bommeli C., Koehler P.;
RT "Primary structure and biochemical properties of a variant-specific surface
RT protein of Giardia.";
RL Mol. Biochem. Parasitol. 86:13-27(1997).
RN [2]
RP CHARACTERIZATION.
RX PubMed=9078242; DOI=10.1042/bj3220049;
RA Papanastasiou P., McConville M.J., Ralton J., Koehler P.;
RT "The variant-specific surface protein of Giardia, VSP4A1, is a glycosylated
RT and palmitoylated protein.";
RL Biochem. J. 322:49-56(1997).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane
CC protein.
CC -!- PTM: O-glycosylated. The major glycan is a trisaccharide with Glc at
CC the reducing terminus.
CC -!- PTM: Palmitoylated.
CC -!- SIMILARITY: Belongs to the Giardia variant surface protein family.
CC {ECO:0000305}.
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DR EMBL; Z83743; CAB06038.1; -; Genomic_DNA.
DR AlphaFoldDB; P92127; -.
DR GlyConnect; 614; 1 O-Linked glycan.
DR SwissPalm; P92127; -.
DR VEuPathDB; GiardiaDB:DHA2_150135; -.
DR VEuPathDB; GiardiaDB:GL50581_2487; -.
DR VEuPathDB; GiardiaDB:GL50581_2604; -.
DR VEuPathDB; GiardiaDB:GL50581_3335; -.
DR VEuPathDB; GiardiaDB:GL50803_00137614; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR CDD; cd00064; FU; 1.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR006212; Furin_repeat.
DR InterPro; IPR005127; Giardia_VSP.
DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR Pfam; PF03302; VSP; 1.
DR SMART; SM00181; EGF; 4.
DR SMART; SM00261; FU; 4.
DR SUPFAM; SSF57184; SSF57184; 4.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Lipoprotein; Membrane; Palmitate; Repeat;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT CHAIN 15..687
FT /note="Variant-specific surface protein VSP4A1"
FT /id="PRO_0000036461"
FT TOPO_DOM 15..660
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 661..681
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 682..687
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 687 AA; 70857 MW; D892F675D626D7EC CRC64;
MLLTAFYVVL GSFAAPCQQD GDHIVTCQVN KCETVGLFEI CTECKTGGVP VDGFCRPFGS
IQAAAAGCTK ADGTALDKTA TTCGKCGDGY FLFMGGCYKT ESQPGSEICT TASNGLCTAC
KVDSQYIFQN KATPSEKGSE CILCWDTTDR NGVMGVANCA TCTAPASSTG PATCTECMAG
TYKKSDTECA ACHSDCATCS GEANNQCTSC ETGKYLKSNQ CVEKNTCNTN HYPDDTSMTC
VACTVLDANC ATCSFDSATA KGKCLTCNSN KIPRTTLDGT STCVENSYAG CQGADNELFM
KEDQSACLLC GDTKEASNDK GVANCRTCTK NANDSPPTCT ACLDGYFLER GSCTTTCADN
CATCSEATTE DKCKICKAGF FLASPGEGKC ISCSDTNNGG IDGCAECTKE PAGPLKCTKC
KPNRKPAGTS DNYTCTEKTC EDPTVCGGTS GACDAIVIDA NGKEHYYCSY CGETNKFPID
GLCTDNKGTN AGCTDHTCSY CAAGFFLYMG GCYKIDTVPG SYMCSKSTTA GVCDTPNANN
RFFVVPKAIS AEQSVLACGN PLGTIAGGNA YVGVEGCSQC TAPDARADGG MAVATCTACE
DGKKPGKSGT GCVACPDANC KSCTMDDVCE ECADGFSLDN GKCVSSGTNK SGLSTGAIAG
ISVAAIVVVG GLVGFLCWWF ICRGKAQ
