ID   VSG4_TRYBR              Reviewed;         476 AA.
AC   P02897;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Variant surface glycoprotein 4;
DE            Short=VSG 4;
DE   AltName: Full=Metacyclic variable antigen;
DE   Flags: Precursor; Fragment;
OS   Trypanosoma brucei rhodesiense.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=31286;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=6593722; DOI=10.1073/pnas.81.21.6642;
RA   Lenardo M.J., Rice-Ficht A.C., Kelly G., Esser K.M., Donelson J.E.;
RT   "Characterization of the genes specifying two metacyclic variable antigen
RT   types in Trypanosoma brucei rhodesiense.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:6642-6646(1984).
CC   -!- FUNCTION: VSG forms a coat on the surface of the parasite. The
CC       trypanosome evades the immune response of the host by expressing a
CC       series of antigenically distinct VSGs from an estimated 1000 VSG genes.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Note=A
CC       soluble form is released from ruptured cells by the action of a PI-PLC.
CC       {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A03392; VMUT4R.
DR   AlphaFoldDB; P02897; -.
DR   SMR; P02897; -.
DR   PRIDE; P02897; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042783; P:evasion of host immune response; IEA:InterPro.
DR   InterPro; IPR001812; Trypano_VSG_A_N_dom.
DR   InterPro; IPR019609; Variant_surf_glycoprt_trypan_C.
DR   Pfam; PF00913; Trypan_glycop; 1.
DR   Pfam; PF10659; Trypan_glycop_C; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Signal; Trypanosomiasis.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..457
FT                   /note="Variant surface glycoprotein 4"
FT                   /id="PRO_0000036441"
FT   PROPEP          458..>476
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000036442"
FT   REGION          367..439
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        369..383
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        387..413
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        425..439
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           457
FT                   /note="GPI-anchor amidated aspartate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        356
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        38..160
FT                   /evidence="ECO:0000250"
FT   DISULFID        144..203
FT                   /evidence="ECO:0000250"
FT   NON_TER         476
SQ   SEQUENCE   476 AA;  50723 MW;  32F8D04053FC1305 CRC64;
     MPTAGAVLLL ATAAVLSRPA AAANEKKPLT ISAAGAVCGF SNELKEVASF AATKVNAYLT
     ETEQLGTLAV DFCAAIFNGN GKPTAGEVYA ALLAAKTQTD RQAQQQKLVT GALLASSLCS
     QQAGHIESFI HVFYQAHRDA TTTCIYKSDE HQRKAADVPC VNNGGGLNKI TITTRATKPQ
     LNSKYKAIVA GTTPNGAAAE KNCKLVNGEQ SQNVFLASQT TNIALKWGDG LLTVPIGDTA
     ISASNWEPNN KDSIASGNYK ECAAALEQVP PRQSTATAAA SKLLKLEAED DPKLEEELIS
     KNSFYGDFPI SNVKITATDF SSLHSKLKSY RKKHTADGHQ LLKAKLQHLE KQMQMNATAC
     KLGAEISSGE PSTAKTTTSG RCEGKAKTAC PKSDYRQREE KDGKEECKPK SGEEQKTQTT
     GAGEGAADKK EEKCKGKLEP ECTKAPECKW EGETCKDSSI LVNKQFTLSM ISAAFM