VSGP_EBOSU
ID VSGP_EBOSU Reviewed; 372 AA.
AC Q7T9E0;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Pre-small/secreted glycoprotein;
DE Short=pre-sGP;
DE Contains:
DE RecName: Full=Small/secreted glycoprotein;
DE Short=sGP;
DE Contains:
DE RecName: Full=Delta-peptide;
DE Flags: Precursor;
GN Name=SGP; Synonyms=GP;
OS Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola
OS virus).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus.
OX NCBI_TaxID=386033;
OH NCBI_TaxID=77231; Epomops franqueti (Franquet's epauleted fruit bat).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=77243; Myonycteris torquata (Little collared fruit bat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=15367603; DOI=10.1128/jvi.78.19.10370-10377.2004;
RA Sanchez A., Lukwiya M., Bausch D., Mahanty S., Sanchez A.J., Wagoner K.D.,
RA Rollin P.E.;
RT "Analysis of human peripheral blood samples from fatal and nonfatal cases
RT of Ebola (Sudan) hemorrhagic fever: cellular responses, virus load, and
RT nitric oxide levels.";
RL J. Virol. 78:10370-10377(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=15047846; DOI=10.1128/jvi.78.8.4330-4341.2004;
RA Towner J.S., Rollin P.E., Bausch D.G., Sanchez A., Crary S.M., Vincent M.,
RA Lee W.F., Spiropoulou C.F., Ksiazek T.G., Lukwiya M., Kaducu F.,
RA Downing R., Nichol S.T.;
RT "Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in
RT an outbreak setting and assessment of patient viral load as a predictor of
RT outcome.";
RL J. Virol. 78:4330-4341(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=16139097; DOI=10.1016/j.virusres.2005.03.028;
RA Sanchez A., Rollin P.E.;
RT "Complete genome sequence of an Ebola virus (Sudan species) responsible for
RT a 2000 outbreak of human disease in Uganda.";
RL Virus Res. 113:16-25(2005).
CC -!- FUNCTION: sGP seems to possess an anti-inflammatory activity as it can
CC reverse the barrier-decreasing effects of TNF alpha. Might therefore
CC contribute to the lack of inflammatory reaction seen during infection
CC in spite the of extensive necrosis and massive virus production. Does
CC not seem to be involved in activation of primary macrophages. Does not
CC seem to interact specifically with neutrophils.
CC {ECO:0000250|UniProtKB:P60170}.
CC -!- FUNCTION: [Delta-peptide]: Viroporin that permeabilizes mammalian cell
CC plasma membranes. It acts by altering permeation of ionic compounds and
CC small molecules. This activity may lead to viral enterotoxic activity.
CC {ECO:0000250|UniProtKB:P60170}.
CC -!- SUBUNIT: sGP is a homodimer; disulfide-linked. The homodimers are
CC linked by two disulfide bonds in a parallel orientation. Delta-peptide
CC is a monomer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Small/secreted glycoprotein]: Secreted.
CC -!- SUBCELLULAR LOCATION: [Delta-peptide]: Secreted {ECO:0000250}.
CC -!- PTM: Pre-sGP is N-glycosylated. This precursor is processed into mature
CC sGP and delta-peptide by host furin or furin-like proteases. The
CC cleavage site corresponds to the furin optimal cleavage sequence [KR]-
CC X-[KR]-R. Both cleavage fragments contain sialic acid, but only the
CC delta-peptide is O-glycosylated (By similarity). {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=295; Note=Partially edited. RNA editing
CC at this position consists of an insertion of one or two adenine
CC nucleotides. The sequence displayed here is the small secreted
CC glycoprotein, derived from the unedited RNA. The sequence derived from
CC the +1A edited gives rise to the full-length transmembrane glycoprotein
CC GP (AC Q7T9D9), the +2A edited RNA gives rise to the super small
CC secreted glycoprotein ssGP (AC P0C772).;
CC -!- SIMILARITY: Belongs to the filoviruses glycoprotein family.
CC {ECO:0000305}.
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DR EMBL; AY316199; AAP88030.1; -; Genomic_RNA.
DR EMBL; AY344234; AAR11464.1; -; Genomic_RNA.
DR EMBL; AY729654; AAU43886.1; -; Genomic_RNA.
DR RefSeq; YP_138524.1; NC_006432.1.
DR SMR; Q7T9E0; -.
DR DNASU; 3160774; -.
DR GeneID; 3160774; -.
DR Proteomes; UP000000277; Genome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW.
DR GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR InterPro; IPR014625; GPC_FiloV.
DR InterPro; IPR002561; GPC_filovir-type_extra_dom.
DR Pfam; PF01611; Filo_glycop; 1.
DR PIRSF; PIRSF036874; GPC_FiloV; 1.
PE 3: Inferred from homology;
KW Cleavage on pair of basic residues; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; RNA editing; Secreted; Signal; Transport;
KW Viral ion channel.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..372
FT /note="Pre-small/secreted glycoprotein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000245090"
FT CHAIN 33..324
FT /note="Small/secreted glycoprotein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000245091"
FT CHAIN 325..372
FT /note="Delta-peptide"
FT /evidence="ECO:0000250"
FT /id="PRO_0000245092"
FT REGION 320..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 324..325
FT /note="Cleavage; by host furin"
FT /evidence="ECO:0000250"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 204
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 208
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 238
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 257
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 268
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT DISULFID 53
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT DISULFID 108..135
FT /evidence="ECO:0000250"
FT DISULFID 121..147
FT /evidence="ECO:0000250"
FT DISULFID 306
FT /note="Interchain"
FT /evidence="ECO:0000250"
SQ SEQUENCE 372 AA; 42584 MW; 19307E2085FBC6A4 CRC64;
MGGLSLLQLP RDKFRKSSFF VWVIILFQKA FSMPLGVVTN STLEVTEIDQ LVCKDHLAST
DQLKSVGLNL EGSGVSTDIP SATKRWGFRS GVPPKVVSYE AGEWAENCYN LEIKKPDGSE
CLPPPPDGVR GFPRCRYVHK AQGTGPCPGD YAFHKDGAFF LYDRLASTVI YRGVNFAEGV
IAFLILAKPK ETFLQSPPIR EAVNYTENTS SYYATSYLEY EIENFGAQHS TTLFKIDNNT
FVRLDRPHTP QFLFQLNDTI HLHQQLSNTT GRLIWTLDAN INADIGEWAF WENKKISPNN
YVEKSCLSKL YRSTRQKTMM RHRRELQREE SPTGPPGSIR TWFQRIPLGW FHCTYQKGKQ
HCRLRIRQKV EE