ID   VSH2_HAEPA              Reviewed;         141 AA.
AC   P36434;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Type II nicking enzyme V.HpaIIP {ECO:0000303|PubMed:12654995};
DE            Short=V.HpaII;
DE            EC=3.1.-.-;
DE   AltName: Full=HpaII very short patch repair endonuclease;
GN   Name=vsr; Synonyms=hpaIIV {ECO:0000303|PubMed:7514149};
OS   Haemophilus parainfluenzae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7514149; DOI=10.1016/0378-1119(94)90348-4;
RA   Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.;
RT   "Organization and sequence of the HpaII restriction-modification system and
RT   adjacent genes.";
RL   Gene 142:9-15(1994).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: May nick HpaII sequences that contain T/G mispairs resulting
CC       from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC       T transition mutations. The very short patch (VSP) repair process
CC       counteracts the mutagenic process by repairing the mismatches in favor
CC       of the G-containing strand. This enzyme is an endonuclease that nicks
CC       double-stranded DNA within the sequence CTGG next to the thymidine
CC       residue that is mismatched to 2'-deoxyguanosine. The incision is
CC       mismatch-dependent and strand-specific. {ECO:0000250|UniProtKB:P09184}.
CC   -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
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DR   EMBL; L17342; AAA20480.1; -; Genomic_DNA.
DR   AlphaFoldDB; P36434; -.
DR   SMR; P36434; -.
DR   REBASE; 162024; V.BsuBS38ORF4087P.
DR   REBASE; 203434; V.Bam1267ORF4054P.
DR   REBASE; 3659; V.HpaIIP.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   CDD; cd00221; Vsr; 1.
DR   InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR   InterPro; IPR007569; DUF559.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   Pfam; PF04480; DUF559; 1.
DR   Pfam; PF03852; Vsr; 1.
DR   PIRSF; PIRSF018267; VSR_endonuc; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR00632; vsr; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..141
FT                   /note="Type II nicking enzyme V.HpaIIP"
FT                   /id="PRO_0000200290"
SQ   SEQUENCE   141 AA;  17029 MW;  5826648756AF64D7 CRC64;
     MDKLTPEQRK KCMKASSKNK GTKPELLLAK YLWALGLRYR KNDRSIFGTP DLSFKRYKIA
     IFIDGEFWHG KDWDIRKYDI KSNKDFWISK IEHNMNRDKK VNDYLISNGW VIFRFWGKDV
     LKNPEKFSLE IQKAIYERCV R