ID   VSH5_DICDI              Reviewed;         337 AA.
AC   P14326; Q54F28;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Vegetative-specific protein H5;
GN   Name=cinB; Synonyms=H5; ORFNames=DDB_G0291121;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 5-337.
RC   STRAIN=AX3;
RA   Singleton C.K.;
RL   Submitted (MAY-1989) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=3243025; DOI=10.1002/dvg.1020090414;
RA   Singleton C.K., McPherson C.E., Manning S.S.;
RT   "Deactivation of gene expression upon the onset of development in
RT   Dictyostelium discoideum.";
RL   Dev. Genet. 9:327-335(1988).
CC   -!- DEVELOPMENTAL STAGE: This protein is expressed in growing cells and
CC       deactivated upon the initiation of development.
CC       {ECO:0000269|PubMed:3243025}.
CC   -!- INDUCTION: By cycloheximide. {ECO:0000269|PubMed:3243025}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA33445.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AAFI02000175; EAL61844.1; -; Genomic_DNA.
DR   EMBL; X15387; CAA33445.1; ALT_INIT; mRNA.
DR   PIR; S07569; S07569.
DR   RefSeq; XP_635362.1; XM_630270.1.
DR   AlphaFoldDB; P14326; -.
DR   SMR; P14326; -.
DR   STRING; 44689.DDB0220110; -.
DR   ESTHER; dicdi-cinbp; Hormone-sensitive_lipase_like.
DR   MEROPS; S09.A97; -.
DR   PaxDb; P14326; -.
DR   EnsemblProtists; EAL61844; EAL61844; DDB_G0291121.
DR   GeneID; 8628010; -.
DR   KEGG; ddi:DDB_G0291121; -.
DR   dictyBase; DDB_G0291121; cinB.
DR   eggNOG; KOG1515; Eukaryota.
DR   HOGENOM; CLU_012494_6_0_1; -.
DR   InParanoid; P14326; -.
DR   OMA; PCLMEEI; -.
DR   PhylomeDB; P14326; -.
DR   PRO; PR:P14326; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..337
FT                   /note="Vegetative-specific protein H5"
FT                   /id="PRO_0000071556"
FT   MOTIF           88..90
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        161
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        261
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        291
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   CONFLICT        199..217
FT                   /note="FETPSYNRFSEKFYLTKEG -> LKHHPIIDLRESINKGS (in Ref. 2;
FT                   CAA33445)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   337 AA;  38555 MW;  1BC87802EEE2D880 CRC64;
     MDPESFGLDK TAIEICNSYM PVLDIEPRLI REGFKSLYDI QKKHISKTKQ YQLNKENGDS
     LMDVHFFYPS GYEQNPVDHK YKAIFYIHGG GFMVDGIKKL PREISDRTNS ILIYPDYGLT
     PEFKYPLGLK QCYQLFTDIM NGNFNPFNDL INDSISIVGE SSGGNFALSL PLMLKLNNST
     FFKKISKVLV YYPITDCNFE TPSYNRFSEK FYLTKEGMKW CWNHYTNNDS ERDEITCCPL
     KATIDQLKDF PETLVITAET DVLSSEGEQF GLKLSNANVK VSVLRILKTI HGFVSLDQTN
     DSIACRVGMD LSMNFLNNIS NNSIINENNN KTLLKLL