ID   VSI3_TRYBB              Reviewed;         532 AA.
AC   P26328;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Variant surface glycoprotein ILTAT 1.23;
DE            Short=VSG;
DE   Flags: Precursor;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate MIAG 206;
RX   PubMed=1942032; DOI=10.1016/0022-2836(91)80178-w;
RA   Carrington M., Miller N., Blum M.L., Roditi I., Wiley D.C., Turner M.J.;
RT   "Variant specific glycoprotein of Trypanosoma brucei consists of two
RT   domains each having an independently conserved pattern of cysteine
RT   residues.";
RL   J. Mol. Biol. 221:823-835(1991).
CC   -!- FUNCTION: VSG forms a coat on the surface of the parasite. The
CC       trypanosome evades the immune response of the host by expressing a
CC       series of antigenically distinct VSGs from an estimated 1000 VSG genes.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Note=A
CC       soluble form is released from ruptured cells by the action of a PI-PLC.
CC       {ECO:0000250}.
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DR   EMBL; X56768; CAA40087.1; -; mRNA.
DR   PIR; S18448; S18448.
DR   AlphaFoldDB; P26328; -.
DR   SMR; P26328; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR025932; Trypano_VSG_B_N_dom.
DR   InterPro; IPR019609; Variant_surf_glycoprt_trypan_C.
DR   InterPro; IPR027446; VSG_C_dom_sf.
DR   Pfam; PF10659; Trypan_glycop_C; 1.
DR   Pfam; PF13206; VSG_B; 1.
DR   SUPFAM; SSF118251; SSF118251; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Signal;
KW   Trypanosomiasis.
FT   SIGNAL          1..23
FT   CHAIN           24..509
FT                   /note="Variant surface glycoprotein ILTAT 1.23"
FT                   /id="PRO_0000036421"
FT   PROPEP          510..532
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036422"
FT   REGION          79..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          408..504
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        408..425
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        426..442
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        444..472
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        473..504
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           509
FT                   /note="GPI-anchor amidated asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        66
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        419
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        509
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   532 AA;  56779 MW;  EED0D72C919396E6 CRC64;
     MFKNINAAVL LLILSTRNDY ANAAAGDNEN LFLDLCNLLE LGKRAVSKLA PTNLGELAYS
     EIQKLNMSLS DDAWKAKFAP KKGKQENSNN ADGAPRSQEK THARNHAWRQ AATDLAGDEG
     DKPTLRLAGL EAVTQVEKLQ YLSALQPLAE RAAAILEQLK TLHSGSAGLT DTNIRQEIQT
     ALYGTGATAP EKTTLQLLKG KGNVGSTRKD ICGQDNTAAK ADTVLAYLFC ICAGHATDSG
     GAIKVCSQTQ PANNKADADV SDAHTHAAAL AGQCHGSDTT NDIKAAEIDS AILEFTSKLK
     AANQKPYFGK YSATGCTGSD AEGICVMFKT TAKGEGKAVK QIPWVLTLHN AAEMIRKQQA
     VNGKIDSLNQ ELQAIQTAAY ALKPQLEMYK RLQQTTEKAR PGKQLTEMQA GECNTHKSNS
     TCPKNNCKWE EKDGKDGKCV ADDSKVTTQG NAPAGAGDGT AGTTTTPNCA SHTDKTKCEE
     ENKGKTTPVC GWRKGKEGES DQDKEMCRNG SFLAKKKFAL SVVSAAFTAL LF