VSIG1_MOUSE
ID VSIG1_MOUSE Reviewed; 407 AA.
AC Q9D2J4; Q9D9J0; Q9DA22;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=V-set and immunoglobulin domain-containing protein 1;
DE AltName: Full=Cell surface A33 antigen;
DE AltName: Full=Glycoprotein A34;
DE Flags: Precursor;
GN Name=Vsig1; Synonyms=Gpa34;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=16405301;
RA Scanlan M.J., Ritter G., Yin B.W., Williams C. Jr., Cohen L.S.,
RA Coplan K.A., Fortunato S.R., Frosina D., Lee S.Y., Murray A.E., Chua R.,
RA Filonenko V.V., Sato E., Old L.J., Jungbluth A.A.;
RT "Glycoprotein A34, a novel target for antibody-based cancer
RT immunotherapy.";
RL Cancer Immun. 6:2-2(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273 AND SER-274, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D2J4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D2J4-2; Sequence=VSP_030028;
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DR EMBL; DQ007336; AAY56125.1; -; mRNA.
DR EMBL; AK006251; BAB24483.1; -; mRNA.
DR EMBL; AK006862; BAB24769.1; -; mRNA.
DR EMBL; AK019565; BAB31795.1; -; mRNA.
DR CCDS; CCDS30443.1; -. [Q9D2J4-1]
DR RefSeq; NP_080379.1; NM_026103.1.
DR AlphaFoldDB; Q9D2J4; -.
DR SMR; Q9D2J4; -.
DR STRING; 10090.ENSMUSP00000033806; -.
DR GlyGen; Q9D2J4; 3 sites.
DR iPTMnet; Q9D2J4; -.
DR PhosphoSitePlus; Q9D2J4; -.
DR MaxQB; Q9D2J4; -.
DR PaxDb; Q9D2J4; -.
DR PRIDE; Q9D2J4; -.
DR ProteomicsDB; 297590; -. [Q9D2J4-1]
DR ProteomicsDB; 297591; -. [Q9D2J4-2]
DR ABCD; Q9D2J4; 44 sequenced antibodies.
DR DNASU; 78789; -.
DR GeneID; 78789; -.
DR KEGG; mmu:78789; -.
DR CTD; 340547; -.
DR MGI; MGI:1926039; Vsig1.
DR eggNOG; ENOG502QU0R; Eukaryota.
DR InParanoid; Q9D2J4; -.
DR BioGRID-ORCS; 78789; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q9D2J4; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9D2J4; protein.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0003382; P:epithelial cell morphogenesis; IMP:MGI.
DR GO; GO:0030277; P:maintenance of gastrointestinal epithelium; IMP:MGI.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000920; Myelin_P0-rel.
DR InterPro; IPR029861; VSIG1.
DR PANTHER; PTHR44974; PTHR44974; 1.
DR Pfam; PF07686; V-set; 1.
DR PRINTS; PR00213; MYELINP0.
DR SMART; SM00409; IG; 2.
DR SMART; SM00408; IGc2; 2.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Phosphoprotein; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..407
FT /note="V-set and immunoglobulin domain-containing protein
FT 1"
FT /id="PRO_0000313574"
FT TOPO_DOM 23..234
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 23..134
FT /note="Ig-like V-type"
FT DOMAIN 145..229
FT /note="Ig-like C2-type"
FT REGION 268..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..382
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 273
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 202
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 221
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 44..118
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 163..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 1..107
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030028"
FT CONFLICT 125
FT /note="H -> D (in Ref. 2; BAB24483/BAB24769)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="N -> K (in Ref. 2; BAB24483/BAB24769)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="S -> T (in Ref. 2; BAB24483)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="E -> K (in Ref. 2; BAB24769)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 407 AA; 44015 MW; B7094F818B868680 CRC64;
MMVFAFWKVF LILNCLAGQV SMVQVTIPDT FVNVTVGSNV TLLCLYTTTE KSLEKLSIQW
SFFHNKEMEE PISIYYSEGG QASAIGQFKD RIIGATNPGN ASITILHMQP ADSGIYICDV
NNPPHFVGKN QGLLDVTVLV KPSKPFCTIQ GRPEAGHPIS LSCLSAFGTP SPLYYWYNIE
GNTIVPVKES FNTATGVLVI GNLTNFEQGY YQCTAINSLG NSSCEIDLTS SHPEVGIIIG
ALVGALIGAA VIICVVYFAR NKVKSKQQKN LNSSTELEPM TKVHHPQQSE AISADGVQLE
GTLPSSIHAG HNTEPTTTAV LEPEYEPNPP LETTTQPDPE PEGSVPVLAP EAEIQPHPEL
DPETETEPEP EPEPKPEPEP EPELEPDPQS GVIIEPLSKA GEDTVKA
