ID   VSM4_TRYBB              Reviewed;         526 AA.
AC   P02896;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Variant surface glycoprotein MITAT 1.4A;
DE   AltName: Full=VSG 117;
DE   Flags: Precursor;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7120401; DOI=10.1016/0022-2836(82)90475-2;
RA   Boothroyd J.C., Paynter C.A., Coleman S.L., Cross G.A.M.;
RT   "Complete nucleotide sequence of complementary DNA coding for a variant
RT   surface glycoprotein from Trypanosoma brucei.";
RL   J. Mol. Biol. 157:547-556(1982).
RN   [2]
RP   PROTEIN SEQUENCE OF 34-503.
RX   PubMed=7120400; DOI=10.1016/0022-2836(82)90474-0;
RA   Allen G., Gurnett L.P., Cross G.A.M.;
RT   "Complete amino acids sequence of a variant surface glycoprotein (VSG 117)
RT   from Trypanosoma brucei.";
RL   J. Mol. Biol. 157:527-546(1982).
CC   -!- FUNCTION: VSG forms a coat on the surface of the parasite. The
CC       trypanosome evades the immune response of the host by expressing a
CC       series of antigenically distinct VSGs from an estimated 1000 VSG genes.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Note=A
CC       soluble form is released from ruptured cells by the action of a PI-PLC.
CC       {ECO:0000250}.
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DR   EMBL; V01387; CAA24677.1; -; mRNA.
DR   EMBL; J01214; AAA30290.1; -; mRNA.
DR   EMBL; J01215; AAA30291.1; -; mRNA.
DR   PIR; A92883; VMUT8B.
DR   AlphaFoldDB; P02896; -.
DR   SMR; P02896; -.
DR   GlyConnect; 615; 5 N-Linked glycans.
DR   iPTMnet; P02896; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042783; P:evasion of host immune response; IEA:InterPro.
DR   InterPro; IPR001812; Trypano_VSG_A_N_dom.
DR   InterPro; IPR019609; Variant_surf_glycoprt_trypan_C.
DR   Pfam; PF00913; Trypan_glycop; 1.
DR   Pfam; PF10659; Trypan_glycop_C; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   GPI-anchor; Lipoprotein; Membrane; Signal; Trypanosomiasis.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000269|PubMed:7120400"
FT   CHAIN           34..503
FT                   /note="Variant surface glycoprotein MITAT 1.4A"
FT                   /id="PRO_0000036435"
FT   PROPEP          504..526
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036436"
FT   REGION          157..193
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        164..178
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           503
FT                   /note="GPI-anchor amidated aspartate"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        453
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:7120400"
FT   DISULFID        47..173
FT                   /evidence="ECO:0000250"
FT   DISULFID        154..215
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   526 AA;  56318 MW;  C73F34BF2B446EE6 CRC64;
     MDCHTKETLG VTQWRRSTML TLSLLYAITP ADGAKEALEY KTWTNHCGLA ATLRKVAGGV
     LTKLKSHISY RKKLEEMETK LRIYALKGDG VGEQKSAEIL ATTAALMRQK ALTPEEANLK
     TALKAAGFAG EGAAAVSSYL MTLGTLTTSG SAHCLSNEGG DGDGKDQLAP KGCRHGTEAD
     FDAGAGPAES EVADSGFAQV PGKQDGANAG QANMCALFTH QATPHSSQGI YITGAQTKPS
     FGYGMLTIGT TDQTIGLKLS DIKGKQADSA QKFWSSCHAA VKAAQDMKAD PALKVDQTLL
     AVLVASPEMA EILKLEAAAS QQKGPEEVTI DLATEKNNYF GTNNNKLEPL WTKIKGQNIV
     DLAATKGSTK ELGTVTDTAE LQKLLSYYYT VNKEEQKKTA EKITKLETEL ADQKGKSPES
     ECNKISEEPK CNEDKICSWH KEVKAGEKHC KFNSTKAKEK GVSVTQTQTA GGTEATTDKC
     KGKLEDTCKK ESNCKWENNA CKDSSILVTK KFALTVVSAA FVALLF