ID   VSM6_TRYBB              Reviewed;         529 AA.
AC   P26334;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Variant surface glycoprotein MITAT 1.6;
DE            Short=VSG;
DE   Flags: Precursor;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate MIAG 151;
RX   PubMed=1942032; DOI=10.1016/0022-2836(91)80178-w;
RA   Carrington M., Miller N., Blum M.L., Roditi I., Wiley D.C., Turner M.J.;
RT   "Variant specific glycoprotein of Trypanosoma brucei consists of two
RT   domains each having an independently conserved pattern of cysteine
RT   residues.";
RL   J. Mol. Biol. 221:823-835(1991).
RN   [2]
RP   GLYCOSYLATION AT ASN-456, AND STRUCTURE OF CARBOHYDRATE.
RX   PubMed=8250900; DOI=10.1006/bbrc.1993.2412;
RA   Strang A.M., Allen A.K., Holder A.A., van Halbeek H.;
RT   "The carbohydrate structures of Trypanosoma brucei brucei MITat 1.6 variant
RT   surface glycoprotein. A re-investigation of the C-terminal glycan.";
RL   Biochem. Biophys. Res. Commun. 196:1430-1439(1993).
CC   -!- FUNCTION: VSG forms a coat on the surface of the parasite. The
CC       trypanosome evades the immune response of the host by expressing a
CC       series of antigenically distinct VSGs from an estimated 1000 VSG genes.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Note=A
CC       soluble form is released from ruptured cells by the action of a PI-PLC.
CC       {ECO:0000250}.
CC   -!- PTM: N-glycosylated; glycan is composed of 6 to 9 mannose residues.
CC       {ECO:0000269|PubMed:8250900}.
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DR   EMBL; X56764; CAA40083.1; -; mRNA.
DR   PIR; S18453; S18453.
DR   AlphaFoldDB; P26334; -.
DR   SMR; P26334; -.
DR   GlyConnect; 616; 8 N-Linked glycans (1 site).
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042783; P:evasion of host immune response; IEA:InterPro.
DR   InterPro; IPR001812; Trypano_VSG_A_N_dom.
DR   InterPro; IPR019609; Variant_surf_glycoprt_trypan_C.
DR   Pfam; PF00913; Trypan_glycop; 1.
DR   Pfam; PF10659; Trypan_glycop_C; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Signal; Trypanosomiasis.
FT   SIGNAL          1..24
FT   CHAIN           25..506
FT                   /note="Variant surface glycoprotein MITAT 1.6"
FT                   /id="PRO_0000036439"
FT   PROPEP          507..529
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036440"
FT   LIPID           506
FT                   /note="GPI-anchor amidated aspartate"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        456
FT                   /note="N-linked (GlcNAc...) (high mannose) asparagine"
FT                   /evidence="ECO:0000269|PubMed:8250900"
FT                   /id="CAR_000142"
FT   DISULFID        39..170
FT                   /evidence="ECO:0000250"
FT   DISULFID        147..214
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   529 AA;  57285 MW;  A1FB291B900502F4 CRC64;
     MAVHRALAAY AISLYVLLPR KSGATDKGAI KFETWEPLCL LTQDFGNLYN RAHKLNLDID
     TYVTAAQADQ LRLQILLSRA SSKIEAAAAA AATAAIAADL AGKAKHVASC KLAATTLTAT
     TGYLHGRIAE FLEVMTAHRG TTNKYGCLSK SRSDNSNSIT DSVANLKDKC KLTVQQISPD
     NKATEQITKA GFTKLTATGG LTSSDLGGSG QAVCMILSTT ASEVVNNGNL DEPVPYAGGY
     LRRKHDLTSD GNDNLATITD SASAPATRAK TDPYLQIWRA FKNLEDCEST FTSGYSRPSP
     ETLKAADETK TAIKNYVVQK EGKYDQATDK EDDYKNLDKI FKDGKDFYPQ KLWDAMDKKD
     LLKDATQTNE IKKLADITDR SELNKVLLYY TRQKEQTLTK ELKEAQEKAT QANQNDAAAK
     AAEDSCNKLV GGEKCNADKK CSYETETDGT KKCKFNATKA EKSGAPVTQA QTVGETEATP
     EKCKGKDAKT CGTTQGCKWE GETCKDSSIL VTKKFALTVV SAAFVALLF