ID   VSN1_LENAE              Reviewed;         166 AA.
AC   P50186;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Type II nicking enzyme V.NaeI {ECO:0000303|PubMed:12654995};
DE            Short=V.NaeI {ECO:0000303|PubMed:7698663};
DE            EC=3.1.-.-;
DE   AltName: Full=NaeI very short patch repair endonuclease;
GN   Name=vsr; Synonyms=naeIV {ECO:0000303|PubMed:7698663};
OS   Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix
OS   aerocolonigenes).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Lentzea.
OX   NCBI_TaxID=68170;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23870 / DSM 40034 / BCRC 13661 / CBS 609.68 / CIP 107109 / JCM
RC   4614 / KCTC 9379 / NBRC 13195 / NCIMB 12944 / NRRL B-3298 / 701;
RX   PubMed=7698663; DOI=10.1016/0378-1119(94)00806-4;
RA   Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E.,
RA   Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.;
RT   "Cloning and expression of the NaeI restriction endonuclease-encoding gene
RT   and sequence analysis of the NaeI restriction-modification system.";
RL   Gene 155:19-25(1995).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: May nick NaeI sequences that contain T/G mispairs resulting
CC       from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC       T transition mutations. The very short patch (VSP) repair process
CC       counteracts the mutagenic process by repairing the mismatches in favor
CC       of the G-containing strand. This enzyme is an endonuclease that nicks
CC       double-stranded DNA within the sequence GCCGGC (C-methylation site
CC       unknown) next to the thymidine residue that is mismatched to 2'-
CC       deoxyguanosine. The incision is mismatch-dependent and strand-specific.
CC       {ECO:0000250|UniProtKB:P09184}.
CC   -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
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DR   EMBL; U09581; AAC43326.1; -; Genomic_DNA.
DR   RefSeq; WP_030468109.1; NZ_JOFI01000013.1.
DR   AlphaFoldDB; P50186; -.
DR   SMR; P50186; -.
DR   REBASE; 165909; V.Nse506ORF6588P.
DR   REBASE; 166485; V.Nse506ORF2622P.
DR   REBASE; 166489; V.Nse506ORF862P.
DR   REBASE; 3678; V.NaeIP.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   CDD; cd00221; Vsr; 1.
DR   InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   Pfam; PF03852; Vsr; 1.
DR   PIRSF; PIRSF018267; VSR_endonuc; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR00632; vsr; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..166
FT                   /note="Type II nicking enzyme V.NaeI"
FT                   /id="PRO_0000200292"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..38
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   166 AA;  18884 MW;  F383BA2DF70C2B1B CRC64;
     MSDKSSRAAA RARAHASGTY PAPLNAGRSR NMQANRRSGT KPEAALRSAL FKLGYRYRKD
     FLLRLGDGVK VKPDIVFTAR KVAVFIDGCF WHVCPDHGRQ PTTNEWYWSP KLRRNVERDR
     TVNQSLTNAG WRVLRVWEHE ELQDAVAAVV DTLHHLEHGF DTSAED