ID   VSN1_NEIMC              Reviewed;         148 AA.
AC   Q9RLM5;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Type II nicking enzyme V.NmeDIP {ECO:0000303|PubMed:12654995};
DE            Short=V.NmeDIP;
DE            EC=3.1.-.-;
DE   AltName: Full=NmeDIP very short patch repair endonuclease;
GN   Name=vsr; Synonyms=nmeDIVP;
OS   Neisseria meningitidis serogroup C.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=135720;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2120 / Serogroup C / Serotype NT;
RX   PubMed=10671450; DOI=10.1128/jb.182.5.1296-1303.2000;
RA   Claus H., Friedrich A., Frosch M., Vogel U.;
RT   "Differential distribution of novel restriction-modification systems in
RT   clonal lineages of Neisseria meningitidis.";
RL   J. Bacteriol. 182:1296-1303(2000).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: May nick NmeDI sequences that contain T/G mispairs resulting
CC       from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC       T transition mutations. The very short patch (VSP) repair process
CC       counteracts the mutagenic process by repairing the mismatches in favor
CC       of the G-containing strand. This enzyme is an endonuclease that nicks
CC       double-stranded DNA within the sequence RTCGGB next to the thymidine
CC       residue that is mismatched to 2'-deoxyguanosine. The incision is
CC       mismatch-dependent and strand-specific. {ECO:0000250|UniProtKB:P09184}.
CC   -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
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DR   EMBL; AJ238948; CAB59896.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RLM5; -.
DR   SMR; Q9RLM5; -.
DR   REBASE; 152666; V.Rph744ORF7P.
DR   REBASE; 152682; V.Rph620ORF371P.
DR   REBASE; 152764; V.Rsp541ORF1071P.
DR   REBASE; 152772; V.Rsp941ORF1071P.
DR   REBASE; 251328; V.BliADL4ORF3338P.
DR   REBASE; 296185; V.Bve83ORF3864P.
DR   REBASE; 4237; V.NmeDIP.
DR   OMA; GCPQHAT; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   CDD; cd00221; Vsr; 1.
DR   InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR   InterPro; IPR007569; DUF559.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   Pfam; PF04480; DUF559; 1.
DR   Pfam; PF03852; Vsr; 1.
DR   PIRSF; PIRSF018267; VSR_endonuc; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR00632; vsr; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..148
FT                   /note="Type II nicking enzyme V.NmeDIP"
FT                   /id="PRO_0000200291"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   148 AA;  17419 MW;  D6AA99657B48499C CRC64;
     MDRLTPEQRK KCMQSNKSKG TKPELALAKA MWALGLRYRK NSGSIFGKPD FSFKKYKVAV
     FVDGEFWHGK DWEQRKAEIK GNREFWIAKI ERNIRRDMEV TGRLKVEGWA VLRFWSNDVV
     KNTTCCAEKV RQAVRDKQGL SKFQTREK