VSP1_ARATH
ID VSP1_ARATH Reviewed; 270 AA.
AC O49195; O23827; O82121; Q39259; Q42000; Q93VJ6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Vegetative storage protein 1;
DE Flags: Precursor;
GN Name=VSP1; OrderedLocusNames=At5g24780; ORFNames=T4C12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C24;
RA Guerineau F., Zhou D.X.;
RT "An Arabidopsis thaliana gene encoding a putative vegetative storage
RT protein (vsp).";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24; TISSUE=Flower bud;
RA Yu D.Y., Quigley F., Mache R.;
RT "High expression of two novel genes in Arabidopsis thaliana flower
RT carpels.";
RL Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower bud;
RX PubMed=8980528; DOI=10.1007/bf00020217;
RA Utsugi S., Sakamoto W., Ogura Y., Murata M., Motoyoshi F.;
RT "Isolation and characterization of cDNA clones corresponding to the genes
RT expressed preferentially in floral organs of Arabidopsis thaliana.";
RL Plant Mol. Biol. 32:759-765(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9747802; DOI=10.1023/a:1006072014605;
RA Utsugi S., Sakamoto W., Murata M., Motoyoshi F.;
RT "Arabidopsis thaliana vegetative storage protein (VSP) genes: gene
RT organization and tissue-specific expression.";
RL Plant Mol. Biol. 38:565-576(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-270.
RC STRAIN=cv. C24; TISSUE=Flower bud;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
CC -!- FUNCTION: May function as somatic storage protein during early seedling
CC development. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O49195-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in leaves and in gynoecia, especially in
CC styles, the basal and distal ends of ovaries and in siliques.
CC -!- INDUCTION: Expression is enhanced during wounding.
CC -!- SIMILARITY: Belongs to the APS1/VSP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA79173.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF043343; AAB97863.1; -; Genomic_DNA.
DR EMBL; X79490; CAA56036.1; -; mRNA.
DR EMBL; D85190; BAA22095.1; -; mRNA.
DR EMBL; AB006777; BAA33446.1; -; Genomic_DNA.
DR EMBL; AL392145; CAC08252.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93360.1; -; Genomic_DNA.
DR EMBL; AF386930; AAK62375.1; -; mRNA.
DR EMBL; AY044328; AAK73269.1; -; mRNA.
DR EMBL; AY072506; AAL66921.1; -; mRNA.
DR EMBL; AY087185; AAM64741.1; -; mRNA.
DR EMBL; Z18377; CAA79173.1; ALT_FRAME; mRNA.
DR PIR; S45062; S45062.
DR RefSeq; NP_568455.1; NM_122387.4. [O49195-1]
DR PDB; 4FYP; X-ray; 1.80 A; A/B=16-270.
DR PDBsum; 4FYP; -.
DR AlphaFoldDB; O49195; -.
DR SMR; O49195; -.
DR BioGRID; 17822; 6.
DR STRING; 3702.AT5G24780.1; -.
DR PaxDb; O49195; -.
DR PRIDE; O49195; -.
DR ProteomicsDB; 242684; -. [O49195-1]
DR EnsemblPlants; AT5G24780.1; AT5G24780.1; AT5G24780. [O49195-1]
DR GeneID; 832547; -.
DR Gramene; AT5G24780.1; AT5G24780.1; AT5G24780. [O49195-1]
DR KEGG; ath:AT5G24780; -.
DR Araport; AT5G24780; -.
DR TAIR; locus:2184585; AT5G24780.
DR eggNOG; ENOG502QU6T; Eukaryota.
DR HOGENOM; CLU_053338_1_1_1; -.
DR InParanoid; O49195; -.
DR OMA; FMEWLIA; -.
DR OrthoDB; 1100106at2759; -.
DR PhylomeDB; O49195; -.
DR BioCyc; ARA:AT5G24780-MON; -.
DR PRO; PR:O49195; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O49195; baseline and differential.
DR Genevisible; O49195; AT.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0003993; F:acid phosphatase activity; IDA:TAIR.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEP:TAIR.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR DisProt; DP02597; -.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR005519; Acid_phosphat_B-like.
DR InterPro; IPR010028; Acid_phosphatase_pln.
DR InterPro; IPR014403; APS1/VSP.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR Pfam; PF03767; Acid_phosphat_B; 1.
DR PIRSF; PIRSF002674; VSP; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01675; plant-AP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Glycoprotein; Reference proteome;
KW Signal; Storage protein.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..270
FT /note="Vegetative storage protein 1"
FT /id="PRO_0000023987"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 1..4
FT /note="MKIL -> MV (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="A -> V (in Ref. 3; BAA22095)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="V -> F (in Ref. 2; CAA56036)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="I -> M (in Ref. 2; CAA56036)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="V -> I (in Ref. 1, 2 and 8)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="P -> A (in Ref. 2; CAA56036)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="E -> V (in Ref. 2; CAA56036)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="H -> Y (in Ref. 3; BAA22095)"
FT /evidence="ECO:0000305"
FT CONFLICT 194..196
FT /note="VTV -> ITI (in Ref. 1, 2 and 8)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="L -> V (in Ref. 2; CAA56036)"
FT /evidence="ECO:0000305"
FT HELIX 57..65
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 80..87
FT /evidence="ECO:0007829|PDB:4FYP"
FT TURN 88..90
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 92..109
FT /evidence="ECO:0007829|PDB:4FYP"
FT STRAND 119..123
FT /evidence="ECO:0007829|PDB:4FYP"
FT TURN 127..129
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 133..136
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 137..142
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 150..156
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 165..176
FT /evidence="ECO:0007829|PDB:4FYP"
FT STRAND 180..188
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 189..191
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 192..201
FT /evidence="ECO:0007829|PDB:4FYP"
FT STRAND 208..213
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 221..234
FT /evidence="ECO:0007829|PDB:4FYP"
FT STRAND 238..246
FT /evidence="ECO:0007829|PDB:4FYP"
FT HELIX 247..250
FT /evidence="ECO:0007829|PDB:4FYP"
FT STRAND 254..260
FT /evidence="ECO:0007829|PDB:4FYP"
SQ SEQUENCE 270 AA; 30262 MW; ABD0AE4387E02BE5 CRC64;
MKILSLSLLL LLAATVSHVQ SSASVPGLIE LLESNTIFGN EAELLEKEGL SINYPNCRSW
HLGVETSNII NFDTVPANCK AYVEDYLITS KQYQYDSKTV NKEAYFYAKG LALKNDTVNV
WIFDLDDTLL SSIPYYAKYG YGTENTAPGA YWSWLESGES TPGLPETLHL YENLLELGIE
PIIISDRWKK LSEVTVENLK AVGVTKWKHL ILKPNGSKLT QVVYKSKVRN SLVKKGYNIV
GNIGDQWADL VEDTPGRVFK LPNPLYYVPS
