ID   VSP1_CRODM              Reviewed;          10 AA.
AC   P0DKX2;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Thrombin-like enzyme Cdc SI;
DE            Short=SVTLE;
DE            EC=3.4.21.-;
DE   AltName: Full=Fibrinogen-clotting enzyme;
DE   AltName: Full=Snake venom serine protease;
DE            Short=SVSP;
DE   Flags: Fragment;
OS   Crotalus durissus cumanensis (South American rattlesnake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus.
OX   NCBI_TaxID=184542;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=23178323; DOI=10.1016/j.toxicon.2012.11.010;
RA   Patino A.C., Pereanez J.A., Gutierrez J.M., Rucavado A.;
RT   "Biochemical and biological characterization of a two serine proteinases
RT   from Colombian Crotalus durissus cumanensis snake venom.";
RL   Toxicon 63:32-43(2013).
CC   -!- FUNCTION: Thrombin-like snake venom serine protease that coagulates
CC       human plasma and bovine fibrinogen by hydrolysis of the alpha chains
CC       (FGA) (minimum coagulation dose is 120 ug on fibrinogen). Has
CC       fibrinogenolytic activities, and degrades preferentially the Aalpha
CC       chain (FGA). Shows amidolytic activity toward N-benzoyl-L-Arg-p-
CC       nitroanilide, has a lower activity than Cdc SII. In vivo, intravenous
CC       injection induces defibrin(ogen)ation and a loss of the righting reflex
CC       and opisthotoxins, together with a typical gyroxin-like effect (18-20
CC       minutes). Subcutaneous injection into the footpads induces moderate
CC       edema. Potentiates local hemorrhagic activity induced by
CC       metalloproteinases (BaP1). {ECO:0000269|PubMed:23178323}.
CC   -!- ACTIVITY REGULATION: Strongly inhibited by PMSF. Not inhibited by EDTA,
CC       aprotinin, pepstatin, leupeptin, and bestatin.
CC       {ECO:0000269|PubMed:23178323}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.034 mM for N-benzoyl-L-Arg-p-nitroanilide
CC         {ECO:0000269|PubMed:23178323};
CC         Vmax=0.038 nmol/min/mg enzyme {ECO:0000269|PubMed:23178323};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=28561.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23178323};
CC   -!- MISCELLANEOUS: Does not induce myotoxicity or hemorrhage, when injected
CC       intramuscularly. Is not lethal when administered either intravenously
CC       or intraperitoneally into mice (maximum dose tested is 20 ug)
CC       (PubMed:23178323). {ECO:0000305|PubMed:23178323}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   SABIO-RK; P0DKX2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Blood coagulation cascade activating toxin; Direct protein sequencing;
KW   Disulfide bond; Fibrinogenolytic toxin; Hemostasis impairing toxin;
KW   Hydrolase; Protease; Secreted; Serine protease; Toxin.
FT   CHAIN           1..>10
FT                   /note="Thrombin-like enzyme Cdc SI"
FT                   /id="PRO_0000421249"
FT   DOMAIN          1..?
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        7..?
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   UNSURE          7
FT                   /note="Assigned by comparison with orthologs"
FT   NON_TER         10
SQ   SEQUENCE   10 AA;  1033 MW;  252FDA4EBB1AA878 CRC64;
     VIGGDECNIN