ID   VSPAL_TRIAB             Reviewed;          11 AA.
AC   P0DJF4;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Thrombin-like enzyme albolabrase;
DE            Short=SVTLE albolabrase;
DE            EC=3.4.21.-;
DE   AltName: Full=Fibrinogen-clotting enzyme;
DE   AltName: Full=Snake venom serine protease;
DE            Short=SVSP;
DE   Flags: Fragment;
OS   Trimeresurus albolabris (White-lipped pit viper) (Cryptelytrops
OS   albolabris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Trimeresurus.
OX   NCBI_TaxID=8765;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   TISSUE=Venom;
RX   PubMed=21983189; DOI=10.1016/j.cbpb.2011.09.009;
RA   Tan N.H., Fung S.Y., Yap Y.H.;
RT   "Isolation and characterization of the thrombin-like enzyme from
RT   Cryptelytrops albolabris (white-lipped tree viper) venom.";
RL   Comp. Biochem. Physiol. 161:79-85(2012).
CC   -!- FUNCTION: Thrombin-like snake venom serine protease. Releases both
CC       fibrinopeptides A and B after 90 min incubation, but only
CC       fibrinopeptides A after 10 min incubation. Clots fibrinogen with
CC       different clotting time depending on the species: bovine > dog = human
CC       > goat. Fails to clot both rabbit and cat fibrinogens.
CC       {ECO:0000269|PubMed:21983189}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.11 mM for N-benzoyl-L-Pro-L-Phe-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:21983189};
CC         KM=0.13 mM for N-benzoyl-L-Phe-L-Val-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:21983189};
CC         KM=0.31 mM for N-benzoyl-L-Val-L-Gly-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:21983189};
CC         KM=0.35 mM for N-p-tosyl-Gly-L-Pro-L-Lys p-nitroanilide
CC         {ECO:0000269|PubMed:21983189};
CC         KM=0.61 mM for N-alpha-benzoyl-L-arginine ethyl ester (BAEE)
CC         {ECO:0000269|PubMed:21983189};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:21983189}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- PTM: Glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
CC       {ECO:0000305}.
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DR   SABIO-RK; P0DJF4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Blood coagulation cascade activating toxin; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hemostasis impairing toxin; Hydrolase;
KW   Protease; Secreted; Serine protease; Toxin.
FT   CHAIN           1..>11
FT                   /note="Thrombin-like enzyme albolabrase"
FT                   /id="PRO_0000416385"
FT   DISULFID        7..?
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   NON_TER         11
SQ   SEQUENCE   11 AA;  1148 MW;  27CD8CDA4EBB1AA8 CRC64;
     VVGGDECNIN E