VSPP_TRIPP
ID VSPP_TRIPP Reviewed; 19 AA.
AC P0DJF7;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=Thrombin-like enzyme purpurase;
DE Short=SVTLE purpurase;
DE EC=3.4.21.-;
DE AltName: Full=Fibrinogen-clotting enzyme;
DE AltName: Full=Snake venom serine protease;
DE Short=SVSP;
DE Flags: Fragment;
OS Trimeresurus purpureomaculatus (Mangrove pit viper) (Cryptelytrops
OS purpureomaculatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Crotalinae; Trimeresurus.
OX NCBI_TaxID=101163;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC TISSUE=Venom;
RX PubMed=19770070; DOI=10.1016/j.cbpc.2009.09.002;
RA Tan N.H.;
RT "Isolation and characterization of the thrombin-like enzyme from
RT Cryptelytrops purpureomaculatus venom.";
RL Comp. Biochem. Physiol. 151:131-136(2010).
CC -!- FUNCTION: Thrombin-like snake venom serine protease. Releases only
CC fibrinopeptides A after 2 hours incubation. Clots fibrinogen with
CC different clotting time depending on the species: cat > human > dog >
CC goat >> rabbit. {ECO:0000269|PubMed:19770070}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.44 mM for N-benzoyl-L-Pro-L-Phe-L-Arg p-nitroanilide
CC {ECO:0000269|PubMed:19770070};
CC KM=0.72 mM for N-benzoyl-L-Phe-L-Val-L-Arg p-nitroanilide
CC {ECO:0000269|PubMed:19770070};
CC KM=0.76 mM for N-benzoyl-L-Val-L-Gly-L-Arg p-nitroanilide
CC {ECO:0000269|PubMed:19770070};
CC KM=0.14 mM for N-p-tosyl-Gly-L-Pro-L-Lys p-nitroanilide
CC {ECO:0000269|PubMed:19770070};
CC KM=0.09 mM for N-alpha-benzoyl-L-arginine ethyl ester (BAEE)
CC {ECO:0000269|PubMed:19770070};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19770070}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- PTM: Glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR AlphaFoldDB; P0DJF7; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Blood coagulation cascade activating toxin; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Hemostasis impairing toxin; Hydrolase;
KW Protease; Secreted; Serine protease; Toxin.
FT CHAIN 1..>19
FT /note="Thrombin-like enzyme purpurase"
FT /id="PRO_0000416396"
FT DOMAIN 1..>19
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 7..?
FT NON_TER 19
SQ SEQUENCE 19 AA; 2143 MW; 5A2B67F343EFE102 CRC64;
VVGGDECNIN DHRSLVRIF