ID   VSPP_TRIPP              Reviewed;          19 AA.
AC   P0DJF7;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Thrombin-like enzyme purpurase;
DE            Short=SVTLE purpurase;
DE            EC=3.4.21.-;
DE   AltName: Full=Fibrinogen-clotting enzyme;
DE   AltName: Full=Snake venom serine protease;
DE            Short=SVSP;
DE   Flags: Fragment;
OS   Trimeresurus purpureomaculatus (Mangrove pit viper) (Cryptelytrops
OS   purpureomaculatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Trimeresurus.
OX   NCBI_TaxID=101163;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   TISSUE=Venom;
RX   PubMed=19770070; DOI=10.1016/j.cbpc.2009.09.002;
RA   Tan N.H.;
RT   "Isolation and characterization of the thrombin-like enzyme from
RT   Cryptelytrops purpureomaculatus venom.";
RL   Comp. Biochem. Physiol. 151:131-136(2010).
CC   -!- FUNCTION: Thrombin-like snake venom serine protease. Releases only
CC       fibrinopeptides A after 2 hours incubation. Clots fibrinogen with
CC       different clotting time depending on the species: cat > human > dog >
CC       goat >> rabbit. {ECO:0000269|PubMed:19770070}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.44 mM for N-benzoyl-L-Pro-L-Phe-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:19770070};
CC         KM=0.72 mM for N-benzoyl-L-Phe-L-Val-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:19770070};
CC         KM=0.76 mM for N-benzoyl-L-Val-L-Gly-L-Arg p-nitroanilide
CC         {ECO:0000269|PubMed:19770070};
CC         KM=0.14 mM for N-p-tosyl-Gly-L-Pro-L-Lys p-nitroanilide
CC         {ECO:0000269|PubMed:19770070};
CC         KM=0.09 mM for N-alpha-benzoyl-L-arginine ethyl ester (BAEE)
CC         {ECO:0000269|PubMed:19770070};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19770070}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- PTM: Glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   AlphaFoldDB; P0DJF7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Blood coagulation cascade activating toxin; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hemostasis impairing toxin; Hydrolase;
KW   Protease; Secreted; Serine protease; Toxin.
FT   CHAIN           1..>19
FT                   /note="Thrombin-like enzyme purpurase"
FT                   /id="PRO_0000416396"
FT   DOMAIN          1..>19
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        7..?
FT   NON_TER         19
SQ   SEQUENCE   19 AA;  2143 MW;  5A2B67F343EFE102 CRC64;
     VVGGDECNIN DHRSLVRIF