ID   VSR_NEIMA               Reviewed;         140 AA.
AC   Q9JWD6; A1IPP5;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Type II nicking enzyme V.NmeAIP {ECO:0000303|PubMed:12654995};
DE            EC=3.1.-.-;
DE   AltName: Full=Very short patch repair endonuclease;
GN   Name=vsr; OrderedLocusNames=NMA0429;
OS   Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 /
OS   Z2491).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122587;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15465 / Z2491;
RX   PubMed=10761919; DOI=10.1038/35006655;
RA   Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M.,
RA   Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M.,
RA   Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K.,
RA   Leather S., Moule S., Mungall K.L., Quail M.A., Rajandream M.A.,
RA   Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G.,
RA   Barrell B.G.;
RT   "Complete DNA sequence of a serogroup A strain of Neisseria meningitidis
RT   Z2491.";
RL   Nature 404:502-506(2000).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: May nick NmeAIP sequences that contain T/G mispairs resulting
CC       from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC       T transition mutations. The very short patch (VSP) repair process
CC       counteracts the mutagenic process by repairing the mismatches in favor
CC       of the G-containing strand. This enzyme is an endonuclease that nicks
CC       double-stranded DNA within the sequence CCGG (C-methylation site
CC       unknown) next to the thymidine residue that is mismatched to 2'-
CC       deoxyguanosine. The incision is mismatch-dependent and strand-specific.
CC       {ECO:0000250|UniProtKB:P09184}.
CC   -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
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DR   EMBL; AL157959; CAM07716.1; -; Genomic_DNA.
DR   PIR; H81959; H81959.
DR   RefSeq; WP_002246501.1; NC_003116.1.
DR   AlphaFoldDB; Q9JWD6; -.
DR   SMR; Q9JWD6; -.
DR   REBASE; 152700; V.Rph841ORF106P.
DR   REBASE; 156150; V.BamRD77ORF3565P.
DR   REBASE; 233039; V.SfrNXT3ORF1642P.
DR   REBASE; 4643; V.NmeAIP.
DR   EnsemblBacteria; CAM07716; CAM07716; NMA0429.
DR   KEGG; nma:NMA0429; -.
DR   HOGENOM; CLU_111913_1_1_4; -.
DR   OMA; HDCYLFK; -.
DR   BioCyc; NMEN122587:NMA_RS02190-MON; -.
DR   Proteomes; UP000000626; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   CDD; cd00221; Vsr; 1.
DR   InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   Pfam; PF03852; Vsr; 1.
DR   PIRSF; PIRSF018267; VSR_endonuc; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR00632; vsr; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..140
FT                   /note="Type II nicking enzyme V.NmeAIP"
FT                   /id="PRO_0000200294"
SQ   SEQUENCE   140 AA;  16751 MW;  49FAE6003EDC12F6 CRC64;
     MTDIFTTSKR SFVMLKIHSK ETKPEVLVRK FLFFQGFRYR KNDKRYVGKP DIVLSKYKTV
     VFIHGCFWYG HSCNKGHIPK SNTDFWLEKI TKNCERDIKN ETELEKIGFK VIVVWECELK
     NKAICRERLN RLVREIKDAV