VSR_RSVT
ID VSR_RSVT Reviewed; 211 AA.
AC P26658;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 07-OCT-2020, entry version 51.
DE RecName: Full=Suppressor of RNA silencing p3;
DE AltName: Full=Protein NS3;
DE AltName: Full=Protein p3;
GN ORFNames=p3;
OS Rice stripe virus (isolate T) (RSV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Phenuiviridae; Tenuivirus.
OX NCBI_TaxID=36394;
OH NCBI_TaxID=4498; Avena sativa (Oat).
OH NCBI_TaxID=66017; Digitaria.
OH NCBI_TaxID=38413; Eragrostis.
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4530; Oryza sativa (Rice).
OH NCBI_TaxID=4555; Setaria italica (Foxtail millet) (Panicum italicum).
OH NCBI_TaxID=4556; Setaria viridis (Green bristlegrass) (Setaria italica subsp. viridis).
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH NCBI_TaxID=4577; Zea mays (Maize).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2016591; DOI=10.1099/0022-1317-72-4-763;
RA Zhu Y., Hayakawa T., Toriyama S., Takahashi M.;
RT "Complete nucleotide sequence of RNA 3 of rice stripe virus: an ambisense
RT coding strategy.";
RL J. Gen. Virol. 72:763-767(1991).
RN [2]
RP RNA-BINDING.
RX PubMed=16025246; DOI=10.1007/s11262-005-1797-7;
RA Liang D., Ma X., Qu Z., Hull R.;
RT "Nucleic acid binding property of the gene products of rice stripe virus.";
RL Virus Genes 31:203-209(2005).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=16025247; DOI=10.1007/s11262-005-1798-6;
RA Liang D., Qu Z., Ma X., Hull R.;
RT "Detection and localization of Rice stripe virus gene products in vivo.";
RL Virus Genes 31:211-221(2005).
RN [4]
RP HOMODIMERIZATION, AND MUTAGENESIS OF ARG-50; LYS-77; ARG-94; LYS-112;
RP ARG-124; LYS-127; LYS-165; ARG-169; 173-LYS-LYS-174; ARG-175 AND ARG-190.
RX PubMed=20951141; DOI=10.1016/j.jmb.2010.10.007;
RA Shen M., Xu Y., Jia R., Zhou X., Ye K.;
RT "Size-independent and noncooperative recognition of dsRNA by the rice
RT stripe virus RNA silencing suppressor NS3.";
RL J. Mol. Biol. 404:665-679(2010).
CC -!- FUNCTION: Acts as a suppressor of RNA-mediated gene silencing, also
CC known as post-transcriptional gene silencing (PTGS), presumably through
CC the binding of dsRNA.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:16025247}.
CC -!- SIMILARITY: Belongs to the tenuiviruses p3 protein family.
CC {ECO:0000305}.
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DR EMBL; X53563; CAA37634.1; -; Genomic_RNA.
DR RefSeq; NP_620547.1; NC_003776.1.
DR SMR; P26658; -.
DR GeneID; 962686; -.
DR KEGG; vg:962686; -.
DR Proteomes; UP000006677; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1440.190; -; 1.
DR InterPro; IPR007974; Tenui_movmnt_prot.
DR InterPro; IPR043105; Tenui_NS3.
DR Pfam; PF05310; Tenui_NS3; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Reference proteome; RNA-binding;
KW Suppressor of RNA silencing.
FT CHAIN 1..211
FT /note="Suppressor of RNA silencing p3"
FT /id="PRO_0000222529"
FT MUTAGEN 50
FT /note="R->G: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 77
FT /note="K->G: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 94
FT /note="R->G: No effect on RNA-binding or suppression of
FT RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 112
FT /note="K->G: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 124
FT /note="R->G: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 127
FT /note="K->G: No effect on RNA-binding or suppression of
FT RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 165
FT /note="K->G: No effect on RNA-binding or suppression of
FT RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 169
FT /note="R->G: No effect on RNA-binding or suppression of
FT RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 173..174
FT /note="KK->GG,ED: Complete loss of RNA-binding and
FT suppression of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 175
FT /note="R->E: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
FT MUTAGEN 190
FT /note="R->G: Complete loss of RNA-binding and suppression
FT of RNA-mediated gene silencing."
FT /evidence="ECO:0000269|PubMed:20951141"
SQ SEQUENCE 211 AA; 23874 MW; 8C06ACA56E604A20 CRC64;
MNVFTSSVGS VEFDHPLLLE NDLTSLSINC DDVHCSSRAL CYIYDIHSSR HPSIDEHQFL
RLLHGPDDAV TLGSFLKTLI WILSHDKNLP EEYRLPTIMM SSSYVKFFTE VKPRPPSTNC
WTCRMSKDNL PFTVPSVKGF PPDAELYIVP ISDHDGKPVK FDNRKTLYRS PSKKRHKYVI
SSDKPPLSAR YVKYVDSSAL ESLPGSSPAV L
