ID   VSR_TRVPP               Reviewed;         141 AA.
AC   Q77JX3;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   02-JUN-2021, entry version 37.
DE   RecName: Full=Suppressor of RNA silencing;
DE   AltName: Full=16kD cysteine rich protein;
DE            Short=P16;
OS   Tobacco rattle virus (isolate PpK20) (TRV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Virgaviridae; Tobravirus.
OX   NCBI_TaxID=652939;
OH   NCBI_TaxID=42337; Bidens pilosa (Hairy beggarticks) (Cobbler's pegs).
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=59895; Cynara cardunculus var. scolymus (Globe artichoke) (Cynara scolymus).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11169199; DOI=10.1046/j.0960-7412.2000.00942.x;
RA   Ratcliff F., Martin-Hernandez A.M., Baulcombe D.C.;
RT   "Technical Advance. Tobacco rattle virus as a vector for analysis of gene
RT   function by silencing.";
RL   Plant J. 25:237-245(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12028572; DOI=10.1046/j.1365-313x.2002.01297.x;
RA   Liu Y., Schiff M., Marathe R., Dinesh-Kumar S.P.;
RT   "Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated
RT   resistance to tobacco mosaic virus.";
RL   Plant J. 30:415-429(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Visser P.B., Bol J.F.;
RT   "Complete nucleotide sequence of RNA 1 of tobacco rattle virus isolate
RT   PpK20.";
RL   Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   PubMed=18272576; DOI=10.1128/jvi.02438-07;
RA   Martin-Hernandez A.M., Baulcombe D.C.;
RT   "Tobacco rattle virus 16-kilodalton protein encodes a suppressor of RNA
RT   silencing that allows transient viral entry in meristems.";
RL   J. Virol. 82:4064-4071(2008).
RN   [5]
RP   NUCLEAR LOCALIZATION SIGNALS, AND SUBCELLULAR LOCATION.
RX   PubMed=18559946; DOI=10.1099/vir.0.83503-0;
RA   Ghazala W., Waltermann A., Pilot R., Winter S., Varrelmann M.;
RT   "Functional characterization and subcellular localization of the 16K
RT   cysteine-rich suppressor of gene silencing protein of tobacco rattle
RT   virus.";
RL   J. Gen. Virol. 89:1748-1758(2008).
CC   -!- FUNCTION: Weak suppressor of RNA-mediated gene silencing, also known as
CC       post-transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that performs sequence-specific inhibition of viral mRNAs
CC       expression. This could be used by the virus to infect efficiently the
CC       host the meristem cells. {ECO:0000269|PubMed:18272576}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:18559946}.
CC       Host nucleus {ECO:0000305}.
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DR   EMBL; AF314165; AAG46034.1; -; Genomic_RNA.
DR   EMBL; AF406990; AAM50511.1; -; Genomic_RNA.
DR   EMBL; AF166084; AAD48029.1; -; Genomic_RNA.
DR   RefSeq; NP_620672.1; NC_003805.1.
DR   GeneID; 962132; -.
DR   KEGG; vg:962132; -.
DR   Proteomes; UP000001669; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007968; Tobacco_rattle_virus_16kDa.
DR   Pfam; PF05304; DUF728; 1.
PE   4: Predicted;
KW   Host cytoplasm; Host nucleus; Reference proteome;
KW   Suppressor of RNA silencing.
FT   CHAIN           1..141
FT                   /note="Suppressor of RNA silencing"
FT                   /id="PRO_0000409290"
FT   REGION          94..117
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           75..91
FT                   /note="Bipartite nuclear localization signal 1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           111..128
FT                   /note="Bipartite nuclear localization signal 2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   141 AA;  16337 MW;  181888373C9CCD00 CRC64;
     MTCVLKGCVN EVTVLGHETC SIGHANKLRK QVADMVGVTR RCAENNCGWF VCVVINDFTF
     DVYNCCGRSH LEKCRKRVET RNREIWKQIR RNQAENMSAT AKKSHNSKTS KKKFKEDREF
     GTPKRFLRDD VPFGIDRLFA F