VSTM4_HUMAN
ID VSTM4_HUMAN Reviewed; 320 AA.
AC Q8IW00; B4DNI6; Q96MX7;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=V-set and transmembrane domain-containing protein 4;
DE Contains:
DE RecName: Full=Peptide Lv;
DE Flags: Precursor;
GN Name=VSTM4; Synonyms=C10orf72;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP SER-68.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS SER-68 AND
RP ARG-243.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP VARIANT [LARGE SCALE ANALYSIS] HIS-100.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Peptide Lv enhances L-type voltage-gated calcium channel (L-
CC VGCC) currents in retinal photoreceptors.
CC {ECO:0000250|UniProtKB:T1NXB5}.
CC -!- INTERACTION:
CC Q8IW00; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-4311759, EBI-3867333;
CC Q8IW00; Q9Y680: FKBP7; NbExp=3; IntAct=EBI-4311759, EBI-3918971;
CC Q8IW00; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-4311759, EBI-11959885;
CC Q8IW00; Q8IUG1: KRTAP1-3; NbExp=3; IntAct=EBI-4311759, EBI-11749135;
CC Q8IW00; P60328: KRTAP12-3; NbExp=3; IntAct=EBI-4311759, EBI-11953334;
CC Q8IW00; Q99750: MDFI; NbExp=3; IntAct=EBI-4311759, EBI-724076;
CC Q8IW00; Q16623: STX1A; NbExp=3; IntAct=EBI-4311759, EBI-712466;
CC Q8IW00; P22735: TGM1; NbExp=3; IntAct=EBI-4311759, EBI-2562368;
CC -!- SUBCELLULAR LOCATION: [Peptide Lv]: Secreted
CC {ECO:0000250|UniProtKB:T1NXB5}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IW00-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IW00-2; Sequence=VSP_022766, VSP_022767;
CC -!- PTM: Proteolytically cleaved to generate a bioactive peptide.
CC {ECO:0000269|PubMed:16959974}.
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DR EMBL; AK056299; BAB71142.1; -; mRNA.
DR EMBL; AK297931; BAG60248.1; -; mRNA.
DR EMBL; AC060234; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084727; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC041414; AAH41414.1; -; mRNA.
DR CCDS; CCDS31198.1; -. [Q8IW00-1]
DR CCDS; CCDS7228.1; -. [Q8IW00-2]
DR RefSeq; NP_001026916.2; NM_001031746.4. [Q8IW00-1]
DR RefSeq; NP_659421.1; NM_144984.3. [Q8IW00-2]
DR AlphaFoldDB; Q8IW00; -.
DR BioGRID; 128223; 12.
DR IntAct; Q8IW00; 10.
DR STRING; 9606.ENSP00000331062; -.
DR GlyGen; Q8IW00; 4 sites.
DR iPTMnet; Q8IW00; -.
DR PhosphoSitePlus; Q8IW00; -.
DR BioMuta; VSTM4; -.
DR DMDM; 334302923; -.
DR MassIVE; Q8IW00; -.
DR MaxQB; Q8IW00; -.
DR PaxDb; Q8IW00; -.
DR PeptideAtlas; Q8IW00; -.
DR PRIDE; Q8IW00; -.
DR ProteomicsDB; 70792; -. [Q8IW00-1]
DR ProteomicsDB; 70793; -. [Q8IW00-2]
DR Antibodypedia; 49933; 58 antibodies from 15 providers.
DR DNASU; 196740; -.
DR Ensembl; ENST00000298454.3; ENSP00000298454.3; ENSG00000165633.13. [Q8IW00-2]
DR Ensembl; ENST00000332853.9; ENSP00000331062.3; ENSG00000165633.13. [Q8IW00-1]
DR GeneID; 196740; -.
DR KEGG; hsa:196740; -.
DR MANE-Select; ENST00000332853.9; ENSP00000331062.3; NM_001031746.5; NP_001026916.2.
DR UCSC; uc001jhf.4; human. [Q8IW00-1]
DR CTD; 196740; -.
DR DisGeNET; 196740; -.
DR GeneCards; VSTM4; -.
DR HGNC; HGNC:26470; VSTM4.
DR HPA; ENSG00000165633; Low tissue specificity.
DR neXtProt; NX_Q8IW00; -.
DR OpenTargets; ENSG00000165633; -.
DR PharmGKB; PA134889917; -.
DR VEuPathDB; HostDB:ENSG00000165633; -.
DR eggNOG; ENOG502QUVT; Eukaryota.
DR GeneTree; ENSGT00390000008566; -.
DR HOGENOM; CLU_060467_0_0_1; -.
DR InParanoid; Q8IW00; -.
DR OMA; WLLFADV; -.
DR OrthoDB; 1141836at2759; -.
DR PhylomeDB; Q8IW00; -.
DR TreeFam; TF331374; -.
DR PathwayCommons; Q8IW00; -.
DR SignaLink; Q8IW00; -.
DR BioGRID-ORCS; 196740; 7 hits in 1077 CRISPR screens.
DR ChiTaRS; VSTM4; human.
DR GenomeRNAi; 196740; -.
DR Pharos; Q8IW00; Tdark.
DR PRO; PR:Q8IW00; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q8IW00; protein.
DR Bgee; ENSG00000165633; Expressed in left adrenal gland cortex and 172 other tissues.
DR Genevisible; Q8IW00; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043542; P:endothelial cell migration; IEA:Ensembl.
DR GO; GO:0001935; P:endothelial cell proliferation; IEA:Ensembl.
DR GO; GO:0097601; P:retina blood vessel maintenance; IEA:Ensembl.
DR GO; GO:0061298; P:retina vasculature development in camera-type eye; IEA:Ensembl.
DR GO; GO:0002040; P:sprouting angiogenesis; IEA:Ensembl.
DR GO; GO:0042311; P:vasodilation; IEA:Ensembl.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Membrane; Reference proteome; Secreted; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..320
FT /note="V-set and transmembrane domain-containing protein 4"
FT /id="PRO_0000274483"
FT PEPTIDE 55..104
FT /note="Peptide Lv"
FT /evidence="ECO:0000250|UniProtKB:T1NXB5"
FT /id="PRO_0000431303"
FT TOPO_DOM 24..180
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 24..155
FT /note="Ig-like"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..127
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 153..191
FT /note="VISLKASEESSFEKTKETWAFFEDLYVYAVLVCCVGILS -> GVSSHPVER
FT GAFLGISLVILPHSLPFTADLTMASFLQSS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022766"
FT VAR_SEQ 192..320
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022767"
FT VARIANT 68
FT /note="F -> S (in dbSNP:rs13088)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_030291"
FT VARIANT 100
FT /note="R -> H (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs138454994)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035539"
FT VARIANT 243
FT /note="K -> R (in dbSNP:rs17854124)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_065188"
FT CONFLICT 195
FT /note="F -> S (in Ref. 1; BAG60248)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 36146 MW; AC5F325CB8B0E1C7 CRC64;
MRLLALAAAA LLARAPAPEV CAALNVTVSP GPVVDYLEGE NATLLCHVSQ KRRKDSLLAV
RWFFAHSFDS QEALMVKMTK LRVVQYYGNF SRSAKRRRLR LLEEQRGALY RLSVLTLQPS
DQGHYVCRVQ EISRHRNKWT AWSNGSSATE MRVISLKASE ESSFEKTKET WAFFEDLYVY
AVLVCCVGIL SILLFMLVIV WQSVFNKRKS RVRHYLVKCP QNSSGETVTS VTSLAPLQPK
KGKRQKEKPD IPPAVPAKAP IAPTFHKPKL LKPQRKVTLP KIAEENLTYA ELELIKPHRA
AKGAPTSTVY AQILFEENKL
