VSTM4_MOUSE
ID VSTM4_MOUSE Reviewed; 319 AA.
AC T1NXB5;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=V-set and transmembrane domain-containing protein 4;
DE Contains:
DE RecName: Full=Peptide Lv;
DE Flags: Precursor;
GN Name=Vstm4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP MASS SPECTROMETRY, SUBCELLULAR LOCATION OF LV PEPTIDE, AND FUNCTION OF LV
RP PEPTIDE.
RX PubMed=22912796; DOI=10.1371/journal.pone.0043091;
RA Shi L., Ko M.L., Abbott L.C., Ko G.Y.;
RT "Identification of Peptide lv, a novel putative neuropeptide that regulates
RT the expression of L-type voltage-gated calcium channels in
RT photoreceptors.";
RL PLoS ONE 7:E43091-E43091(2012).
CC -!- FUNCTION: Peptide Lv enhances L-type voltage-gated calcium channel (L-
CC VGCC) currents in retinal photoreceptors.
CC {ECO:0000269|PubMed:22912796}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Peptide Lv]: Secreted
CC {ECO:0000269|PubMed:22912796}.
CC -!- TISSUE SPECIFICITY: Peptide Lv is widely expressed in various tissues
CC and the central nervous system, including the retinal photoreceptor
CC layer, hippocampus, olfactory bulb, and cerebellum.
CC {ECO:0000269|PubMed:22912796}.
CC -!- PTM: Proteolytically cleaved to generate a bioactive peptide.
CC {ECO:0000269|PubMed:22912796}.
CC -!- MASS SPECTROMETRY: [Peptide Lv]: Mass=5808.66; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:22912796};
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DR EMBL; AK053685; BAC35473.1; -; mRNA.
DR EMBL; AC133585; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466573; EDL24847.1; -; Genomic_DNA.
DR CCDS; CCDS26923.1; -.
DR RefSeq; NP_848906.1; NM_178791.4.
DR AlphaFoldDB; T1NXB5; -.
DR STRING; 10090.ENSMUSP00000055178; -.
DR GlyGen; T1NXB5; 3 sites.
DR iPTMnet; T1NXB5; -.
DR PhosphoSitePlus; T1NXB5; -.
DR PaxDb; T1NXB5; -.
DR PRIDE; T1NXB5; -.
DR ProteomicsDB; 297613; -.
DR Antibodypedia; 49933; 58 antibodies from 15 providers.
DR DNASU; 320736; -.
DR Ensembl; ENSMUST00000053175; ENSMUSP00000055178; ENSMUSG00000050666.
DR GeneID; 320736; -.
DR KEGG; mmu:320736; -.
DR UCSC; uc007szj.1; mouse.
DR CTD; 196740; -.
DR MGI; MGI:2444633; Vstm4.
DR VEuPathDB; HostDB:ENSMUSG00000050666; -.
DR eggNOG; ENOG502QUVT; Eukaryota.
DR GeneTree; ENSGT00390000008566; -.
DR HOGENOM; CLU_060467_0_0_1; -.
DR OMA; WLLFADV; -.
DR OrthoDB; 1141836at2759; -.
DR BioGRID-ORCS; 320736; 3 hits in 72 CRISPR screens.
DR PRO; PR:T1NXB5; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; T1NXB5; protein.
DR Bgee; ENSMUSG00000050666; Expressed in otolith organ and 157 other tissues.
DR Genevisible; T1NXB5; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043542; P:endothelial cell migration; IDA:MGI.
DR GO; GO:0001935; P:endothelial cell proliferation; IMP:MGI.
DR GO; GO:0097601; P:retina blood vessel maintenance; IMP:MGI.
DR GO; GO:0061298; P:retina vasculature development in camera-type eye; IDA:MGI.
DR GO; GO:0002040; P:sprouting angiogenesis; IDA:MGI.
DR GO; GO:0042311; P:vasodilation; IDA:MGI.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Reference proteome; Secreted; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..319
FT /note="V-set and transmembrane domain-containing protein 4"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431304"
FT PEPTIDE 55..103
FT /note="Peptide Lv"
FT /evidence="ECO:0000269|PubMed:22912796"
FT /id="PRO_0000431305"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 24..154
FT /note="Ig-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..126
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
SQ SEQUENCE 319 AA; 35899 MW; F35E3F2D94159761 CRC64;
MRLRLLALAA AVLLGPAPEV CGALNVTVSP GPVVDYLEGE NATLLCHVSQ KRRKDSLLAV
RWFFAPDGSQ EALMVKMTKL RIIQYYGNFS RTANQQRLRL LEERRGVLYR LSVLTLRPTD
QGQYVCKVQE ISKHRNKWTA WSNGSSATEM RVISLKAGED SSFEKKKVTW AFFEDLYVYA
VLVCCVGILS VLLFTLVIAW QSVFHKRKSR VRHYLVKCPQ NSSGETVTSV TSLAPLQPQK
GKRQKKKVDV PPAVPAKAPI ATTFHKPKLL KPQRKVALPK ITEENLTYAE LELIKPHRAA
KGVPTSTVYA QILFEENQL
