VSTX3_PARSR
ID VSTX3_PARSR Reviewed; 34 AA.
AC P0DL74;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2017, sequence version 1.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Voltage sensor toxin 3 {ECO:0000303|PubMed:24211312};
DE Short=VSTX3 {ECO:0000303|PubMed:24211312};
DE AltName: Full=Beta/kappa-theraphotoxin-Gr4a {ECO:0000250|UniProtKB:P0C2P5};
DE Short=Beta/kappa-TRTX-Gr4a {ECO:0000250|UniProtKB:P0C2P5};
OS Paraphysa scrofa (Chilean copper tarantula) (Phrixotrichus auratus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Paraphysa.
OX NCBI_TaxID=269635;
RN [1]
RP PROTEIN SEQUENCE, SYNTHESIS, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=24211312; DOI=10.1016/j.toxicon.2013.10.029;
RA Cherki R.S., Kolb E., Langut Y., Tsveyer L., Bajayo N., Meir A.;
RT "Two tarantula venom peptides as potent and differential Na(V) channels
RT blockers.";
RL Toxicon 77:58-67(2014).
CC -!- FUNCTION: Potent voltage-gated sodium channel blocker (IC(50)=190 nM
CC and 210 nM on human and rat Nav1.3/SCN3A respectively, 430 nM on human
CC Nav1.7/SCN9A, 770 nM and 290 nM on human and rat Nav1.8/SCN10A
CC respectively) (PubMed:24211312). Binds the voltage-sensor domain of the
CC potassium channel KvAP (from Aeropyrum pernix) and weakly inhibits this
CC channel (By similarity). {ECO:0000250|UniProtKB:P0C2P5,
CC ECO:0000269|PubMed:24211312}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24211312}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:24211312}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000250}.
CC -!- MASS SPECTROMETRY: Mass=4168.0; Method=Electrospray; Note=Monoisotopic
CC mass.; Evidence={ECO:0000269|PubMed:24211312};
CC -!- MISCELLANEOUS: Shows only a very weak inhibition on human Nav1.5/SCN5A.
CC {ECO:0000269|PubMed:24211312}.
CC -!- MISCELLANEOUS: The primary structure of the mature peptide is identical
CC to that of VSTX3 from Grammostola rosea (AC P0C2P5). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 61 (VSTX3)
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; P0DL74; -.
DR SMR; P0DL74; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR011696; Huwentoxin-1.
DR InterPro; IPR013140; Huwentoxin_CS1.
DR Pfam; PF07740; Toxin_12; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Knottin; Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT PEPTIDE 1..34
FT /note="Voltage sensor toxin 3"
FT /evidence="ECO:0000269|PubMed:24211312"
FT /id="PRO_0000442000"
FT DISULFID 2..17
FT /evidence="ECO:0000250|UniProtKB:P0DL72"
FT DISULFID 9..22
FT /evidence="ECO:0000250|UniProtKB:P0DL72"
FT DISULFID 16..29
FT /evidence="ECO:0000250|UniProtKB:P0DL72"
SQ SEQUENCE 34 AA; 4178 MW; BE2D51E87DF93738 CRC64;
DCLGWFKGCD PDNDKCCEGY KCNRRDKWCK YKLW