VSX2_XANOR
ID VSX2_XANOR Reviewed; 136 AA.
AC Q56829; Q5H5B0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Type II nicking enzyme V.XorIIP {ECO:0000303|PubMed:15673718};
DE EC=3.1.-.-;
DE AltName: Full=V.XorII;
DE AltName: Full=XorII very short patch repair endonuclease;
GN Name=vsr {ECO:0000303|PubMed:8078464}; Synonyms=xorIIV;
GN OrderedLocusNames=XOO0606;
OS Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=291331;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JW89011;
RX PubMed=8078464; DOI=10.1007/bf00286690;
RA Choi S.H., Leach J.E.;
RT "Identification of the XorII methyltransferase gene and a vsr homolog from
RT Xanthomonas oryzae pv. oryzae.";
RL Mol. Gen. Genet. 244:383-390(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KACC10331 / KXO85;
RX PubMed=15673718; DOI=10.1093/nar/gki206;
RA Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S.,
RA Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H.,
RA Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S.,
RA Go S.-J.;
RT "The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the
RT bacterial blight pathogen of rice.";
RL Nucleic Acids Res. 33:577-586(2005).
RN [3]
RP NOMENCLATURE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: May nick XorII sequences that contain T/G mispairs resulting
CC from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC T transition mutations. The very short patch (VSP) repair process
CC counteracts the mutagenic process by repairing the mismatches in favor
CC of the G-containing strand. This enzyme is an endonuclease that nicks
CC double-stranded DNA within the sequence CGATCG (C-methylation site
CC unknown) next to the thymidine residue that is mismatched to 2'-
CC deoxyguanosine. The incision is mismatch-dependent and strand-specific.
CC {ECO:0000250|UniProtKB:P09184}.
CC -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW73860.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U06424; AAA50431.1; -; Genomic_DNA.
DR EMBL; AE013598; AAW73860.1; ALT_INIT; Genomic_DNA.
DR PIR; S46292; S46292.
DR RefSeq; WP_011257516.1; NC_006834.1.
DR AlphaFoldDB; Q56829; -.
DR SMR; Q56829; -.
DR REBASE; 101150; V.Rga602ORF33P.
DR REBASE; 10810; V.XorKIP.
DR REBASE; 203804; V.Keu1446ORF816P.
DR REBASE; 3690; V.XorIIP.
DR EnsemblBacteria; AAW73860; AAW73860; XOO0606.
DR KEGG; xoo:XOO0606; -.
DR HOGENOM; CLU_111913_1_1_6; -.
DR Proteomes; UP000006735; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR CDD; cd00221; Vsr; 1.
DR InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR Pfam; PF03852; Vsr; 1.
DR PIRSF; PIRSF018267; VSR_endonuc; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR00632; vsr; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome; Restriction system.
FT CHAIN 1..136
FT /note="Type II nicking enzyme V.XorIIP"
FT /id="PRO_0000200293"
FT CONFLICT 36
FT /note="G -> A (in Ref. 1; AAA50431)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 136 AA; 15884 MW; 0EE4EB51958D9E3B CRC64;
MTDRLSPERR RYLMQQVRSK NTRPEKAVRS LLHSIGYRFR LHRKDLPGTP DIVFPSRRLV
LFVHGCFWHG HGCRIGQLPK SRLDYWSPKI EANRARDQRK EAALAAEGWR VAVVWQCELS
DLGALEARLR NILDPS