ID   VSX2_XANOR              Reviewed;         136 AA.
AC   Q56829; Q5H5B0;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Type II nicking enzyme V.XorIIP {ECO:0000303|PubMed:15673718};
DE            EC=3.1.-.-;
DE   AltName: Full=V.XorII;
DE   AltName: Full=XorII very short patch repair endonuclease;
GN   Name=vsr {ECO:0000303|PubMed:8078464}; Synonyms=xorIIV;
GN   OrderedLocusNames=XOO0606;
OS   Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=291331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JW89011;
RX   PubMed=8078464; DOI=10.1007/bf00286690;
RA   Choi S.H., Leach J.E.;
RT   "Identification of the XorII methyltransferase gene and a vsr homolog from
RT   Xanthomonas oryzae pv. oryzae.";
RL   Mol. Gen. Genet. 244:383-390(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KACC10331 / KXO85;
RX   PubMed=15673718; DOI=10.1093/nar/gki206;
RA   Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S.,
RA   Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H.,
RA   Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S.,
RA   Go S.-J.;
RT   "The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the
RT   bacterial blight pathogen of rice.";
RL   Nucleic Acids Res. 33:577-586(2005).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: May nick XorII sequences that contain T/G mispairs resulting
CC       from m5C-deamination. If unrepaired, these mismatches can lead to C-to-
CC       T transition mutations. The very short patch (VSP) repair process
CC       counteracts the mutagenic process by repairing the mismatches in favor
CC       of the G-containing strand. This enzyme is an endonuclease that nicks
CC       double-stranded DNA within the sequence CGATCG (C-methylation site
CC       unknown) next to the thymidine residue that is mismatched to 2'-
CC       deoxyguanosine. The incision is mismatch-dependent and strand-specific.
CC       {ECO:0000250|UniProtKB:P09184}.
CC   -!- SIMILARITY: Belongs to the Vsr family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW73860.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U06424; AAA50431.1; -; Genomic_DNA.
DR   EMBL; AE013598; AAW73860.1; ALT_INIT; Genomic_DNA.
DR   PIR; S46292; S46292.
DR   RefSeq; WP_011257516.1; NC_006834.1.
DR   AlphaFoldDB; Q56829; -.
DR   SMR; Q56829; -.
DR   REBASE; 101150; V.Rga602ORF33P.
DR   REBASE; 10810; V.XorKIP.
DR   REBASE; 203804; V.Keu1446ORF816P.
DR   REBASE; 3690; V.XorIIP.
DR   EnsemblBacteria; AAW73860; AAW73860; XOO0606.
DR   KEGG; xoo:XOO0606; -.
DR   HOGENOM; CLU_111913_1_1_6; -.
DR   Proteomes; UP000006735; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   CDD; cd00221; Vsr; 1.
DR   InterPro; IPR004603; DNA_mismatch_endonuc_vsr.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   Pfam; PF03852; Vsr; 1.
DR   PIRSF; PIRSF018267; VSR_endonuc; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR00632; vsr; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome; Restriction system.
FT   CHAIN           1..136
FT                   /note="Type II nicking enzyme V.XorIIP"
FT                   /id="PRO_0000200293"
FT   CONFLICT        36
FT                   /note="G -> A (in Ref. 1; AAA50431)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   136 AA;  15884 MW;  0EE4EB51958D9E3B CRC64;
     MTDRLSPERR RYLMQQVRSK NTRPEKAVRS LLHSIGYRFR LHRKDLPGTP DIVFPSRRLV
     LFVHGCFWHG HGCRIGQLPK SRLDYWSPKI EANRARDQRK EAALAAEGWR VAVVWQCELS
     DLGALEARLR NILDPS