VTC2_SCHPO
ID VTC2_SCHPO Reviewed; 734 AA.
AC O13718;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Vacuolar transporter chaperone 2;
GN Name=vtc2; ORFNames=SPAC14C4.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181; THR-529 AND TYR-583, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the vacuolar transporter chaperone (VTC)
CC complex, which plays a role in vacuolar membrane fusion. {ECO:0000250}.
CC -!- SUBUNIT: The VTC complex is an integral membrane heterooligomer
CC composed of at least vtc1, vtc2 and vtc4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VTC2/3 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB11204.1; -; Genomic_DNA.
DR PIR; T37696; T37696.
DR RefSeq; NP_594916.1; NM_001020348.2.
DR AlphaFoldDB; O13718; -.
DR SMR; O13718; -.
DR BioGRID; 277933; 15.
DR STRING; 4896.SPAC14C4.11.1; -.
DR iPTMnet; O13718; -.
DR MaxQB; O13718; -.
DR PaxDb; O13718; -.
DR PRIDE; O13718; -.
DR EnsemblFungi; SPAC14C4.11.1; SPAC14C4.11.1:pep; SPAC14C4.11.
DR GeneID; 2541428; -.
DR KEGG; spo:SPAC14C4.11; -.
DR PomBase; SPAC14C4.11; vtc2.
DR VEuPathDB; FungiDB:SPAC14C4.11; -.
DR eggNOG; KOG1161; Eukaryota.
DR eggNOG; KOG4580; Eukaryota.
DR HOGENOM; CLU_009308_2_0_1; -.
DR InParanoid; O13718; -.
DR OMA; SFKFWIH; -.
DR PhylomeDB; O13718; -.
DR PRO; PR:O13718; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0033254; C:vacuolar transporter chaperone complex; ISO:PomBase.
DR GO; GO:0006914; P:autophagy; ISO:PomBase.
DR GO; GO:0006112; P:energy reserve metabolic process; IC:PomBase.
DR GO; GO:0006799; P:polyphosphate biosynthetic process; ISO:PomBase.
DR GO; GO:0007034; P:vacuolar transport; ISO:PomBase.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; ISO:PomBase.
DR Gene3D; 3.20.100.30; -; 1.
DR InterPro; IPR003807; DUF202.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR018966; VTC_domain.
DR InterPro; IPR042267; VTC_sf.
DR Pfam; PF02656; DUF202; 1.
DR Pfam; PF09359; VTC; 1.
DR PROSITE; PS51382; SPX; 1.
PE 1: Evidence at protein level;
KW Chaperone; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..734
FT /note="Vacuolar transporter chaperone 2"
FT /id="PRO_0000116686"
FT TOPO_DOM 1..624
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 625..645
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 646..650
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 651..671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 672..693
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 694..714
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 715..734
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT DOMAIN 1..144
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT MOD_RES 181
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 529
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 583
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 734 AA; 85445 MW; 2373306038C7F4EE CRC64;
MRFSDSIEAG IYEPWRDKYM NYPELKHLLK TEEEAPSWGE NDESKFVSVM DAQLEKVYAF
HLEILKELNE SVDWVKSKVS ASQEPDGPPI SKEEAIKLLE RLDSCTETVK KLEKYTRLNL
TGFFKIVKKH DKLYPGYSLR PVFQVRLRAC PLGSVQFNPL LAEIFSLYNT LRDGLSAPSN
SVQVKPKHEH NVDYNSSMYR RRTFRFWVHP DNVMEVKTYI MRHLPVLYYS GKQGFDKDQN
GVSGILDPIS TCLYLDNSNF DLYSQNLERS EQAYSLRLHW YGKLTPKTDI IVERMVRQGS
TLSHSEDRFT IREKKVRELL SGRYDFRKVE DDHSTTASDQ KKKLIEDVEQ LIVDNHLQPV
LRSVYTRTAF QIPGDDEVRI NLDSDWVMIR EDSLDIERPC RDPEDWHRHD IDDADFPYKH
LRKGEYSRFP YSVLEIRECV RYDEDEPLWI SELRNSHLIS EIDGFSKYEH GVAILFEKYV
SLLPMWVFSM DQDIRKDLQE VYSHPEGSAG SRNVYIKRRN QRVLKQNMTP EPSQPSPLVN
RLKANEMHPV SEEPEDNREV YRNEHGDHFN FRSIPGLLKP STYGSFKHHG KTFVTPPHIK
KPEIPLRVSG PIKVEAKVWL ANERTFLKWL HVVVLLGSLA LALYNSAGER LGQAFGVVYT
LLAIFIGFYA WKLHAKRSQM IKSRSPAPMT DYWGPLIVGT ALAISLIVNM SFALKDAVYQ
NLIEPDRLLV KLFT
