VTC4_SCHPO
ID VTC4_SCHPO Reviewed; 721 AA.
AC P78810; Q1L850;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2000, sequence version 2.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Vacuolar transporter chaperone 4;
GN Name=vtc4; ORFNames=SPCC1322.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 192-721.
RC STRAIN=PR745;
RX PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT "Identification of open reading frames in Schizosaccharomyces pombe
RT cDNAs.";
RL DNA Res. 4:363-369(1997).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495; THR-497; SER-501;
RP THR-534 AND SER-546, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the vacuolar transporter chaperone (VTC)
CC complex, which plays a role in vacuolar membrane fusion. {ECO:0000250}.
CC -!- SUBUNIT: The VTC complex is an integral membrane heterooligomer
CC composed of at least vtc1, vtc2 and vtc4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the VTC4 family. {ECO:0000305}.
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DR EMBL; CU329672; CAA22867.1; -; Genomic_DNA.
DR EMBL; D89159; BAA13821.1; -; mRNA.
DR PIR; T40945; T40945.
DR PIR; T42520; T42520.
DR RefSeq; NP_588142.1; NM_001023132.2.
DR AlphaFoldDB; P78810; -.
DR SMR; P78810; -.
DR BioGRID; 275369; 7.
DR STRING; 4896.SPCC1322.14c.1; -.
DR iPTMnet; P78810; -.
DR MaxQB; P78810; -.
DR PaxDb; P78810; -.
DR PRIDE; P78810; -.
DR EnsemblFungi; SPCC1322.14c.1; SPCC1322.14c.1:pep; SPCC1322.14c.
DR GeneID; 2538788; -.
DR KEGG; spo:SPCC1322.14c; -.
DR PomBase; SPCC1322.14c; vtc4.
DR VEuPathDB; FungiDB:SPCC1322.14c; -.
DR eggNOG; KOG1161; Eukaryota.
DR eggNOG; KOG4580; Eukaryota.
DR HOGENOM; CLU_009308_0_0_1; -.
DR InParanoid; P78810; -.
DR OMA; AGGKQQN; -.
DR PhylomeDB; P78810; -.
DR PRO; PR:P78810; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0033254; C:vacuolar transporter chaperone complex; ISO:PomBase.
DR GO; GO:0006112; P:energy reserve metabolic process; IC:PomBase.
DR GO; GO:0016237; P:lysosomal microautophagy; ISO:PomBase.
DR GO; GO:0006799; P:polyphosphate biosynthetic process; ISO:PomBase.
DR GO; GO:0006797; P:polyphosphate metabolic process; IBA:GO_Central.
DR GO; GO:0007034; P:vacuolar transport; ISO:PomBase.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; ISO:PomBase.
DR Gene3D; 3.20.100.30; -; 1.
DR InterPro; IPR003807; DUF202.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR018966; VTC_domain.
DR InterPro; IPR042267; VTC_sf.
DR Pfam; PF02656; DUF202; 1.
DR Pfam; PF09359; VTC; 1.
DR PROSITE; PS51382; SPX; 1.
PE 1: Evidence at protein level;
KW Chaperone; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..721
FT /note="Vacuolar transporter chaperone 4"
FT /id="PRO_0000363396"
FT TOPO_DOM 1..631
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 632..652
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 653..657
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 658..678
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 679..697
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 698..718
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 719..721
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT DOMAIN 1..146
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REGION 490..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 490..515
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 495
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 497
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 501
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 534
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 546
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT CONFLICT 580
FT /note="K -> R (in Ref. 2; BAA13821)"
FT /evidence="ECO:0000305"
FT CONFLICT 714
FT /note="N -> Y (in Ref. 2; BAA13821)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 721 AA; 83635 MW; DF4471E8FC0A6BCF CRC64;
MKFGQLLKET LMYEYKYSYV NYDKLKKEIK RRNDQGGWSE EDESDFVELL EKELDKVYSF
QKNKSAEVME RIRFCEEQTD EVVRRLDSDN PPNENDFAIL ETELTDIMAT VHDLAKFSEL
NYTAFYKIIK KHDKHTGWIL KPVFAARLNA KPFFKEQYDL LVVKLSKLYD FVRTRGSPIK
GDSAAGGTQQ NFVRQTTKYW VHPNNVTELK IYILKHLPVL VFNPNKEFAR EDAAITSIYY
DNDDLDFYLG RLEKREGAEA IRLRWYGNMD NNNIFVERKT HREDWTGEKS VKARFPLKEK
YVNAFLRGDY TVEEAFAKMR KDGKKSLKEI ESLERLAKEV QYTVLSRGMK PYVRSFYERT
AFQLPGDARV RISLDTNLSL IREDGPSRAG NNWRRMDIGI DYPFDQLPDE DIVRFPYAIL
EVKLQTQFGQ DPPEWVNNLV NSHLVEAVPK FSKFIHGVST LFYDRVTLLP YWFPQMDIDI
RKPATHTFIQ GRSQSGTHSS SVSANVLTDS ENTPIHADGD NYVDEESRIG SSSTRNDNST
FQTSDSFQEL DERTNLLDIS KRKGRDSFVA ALNSRLKDIK DSFFLETVPK FEEPTEPTVI
YEQKYVSPPG KRIYVPVRVE PKTYFALERT YLDYLRYSIL MGSIGITLFS FAKTRSGILG
AASFTLVALF AIFYSTFLYL WRAVNIAKHN AVRYDDRFGP TAICVITFAA ISANVILNFN
A
