ID   VTF3S_VACCW             Reviewed;         288 AA.
AC   P68720; O57221; Q80HV9;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   23-FEB-2022, entry version 46.
DE   RecName: Full=Intermediate transcription factor 3 small subunit;
DE   AltName: Full=VITF-3 32 kDa subunit;
DE   AltName: Full=VITF-3 small subunit;
GN   Name=VITF3S; OrderedLocusNames=VACWR127; ORFNames=A8R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=10077573; DOI=10.1073/pnas.96.6.2692;
RA   Sanz P., Moss B.;
RT   "Identification of a transcription factor, encoded by two vaccinia virus
RT   early genes, that regulates the intermediate stage of viral gene
RT   expression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:2692-2697(1999).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=15471883; DOI=10.1074/jbc.m411033200;
RA   Katsafanas G.C., Moss B.;
RT   "Vaccinia virus intermediate stage transcription is complemented by Ras-
RT   GTPase-activating protein SH3 domain-binding protein (G3BP) and cytoplasmic
RT   activation/proliferation-associated protein (p137) individually or as a
RT   heterodimer.";
RL   J. Biol. Chem. 279:52210-52217(2004).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from
CC       intermediate gene promoters. {ECO:0000269|PubMed:10077573,
CC       ECO:0000269|PubMed:15471883}.
CC   -!- SUBUNIT: Heterodimer of a 45 kDa (A23R) and a 32 kDa (A8R) subunit to
CC       form the virus intermediate transcription factor (VITF)-3.
CC       {ECO:0000269|PubMed:10077573}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:15471883}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A8 family. {ECO:0000305}.
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DR   EMBL; AY243312; AAO89406.1; -; Genomic_DNA.
DR   PIR; T37395; T37395.
DR   RefSeq; YP_233009.1; NC_006998.1.
DR   DNASU; 3707525; -.
DR   GeneID; 3707525; -.
DR   KEGG; vg:3707525; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   InterPro; IPR006834; Pox_A8.
DR   Pfam; PF04745; Pox_A8; 1.
PE   1: Evidence at protein level;
KW   Activator; Early protein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..288
FT                   /note="Intermediate transcription factor 3 small subunit"
FT                   /id="PRO_0000099192"
SQ   SEQUENCE   288 AA;  33575 MW;  49E0C362F8372235 CRC64;
     MFEPVPDLNL EASVELGEVN IDQTTPMIKE NSGFISRSRR LFAHRSKDDE RKLALRFFLQ
     RLYFLDHREI HYLFRCVDAV KDVTITKKNN IIVAPYIALL TIASKGCKLT ETMIEAFFPE
     LYNEHSKKFK FNSQVSIIQE KLGYQFGNYH VYDFEPYYST VALAIRDEHS SGIFNIRQES
     YLVSSLSEIT YRFYLINLKS DLVQWSASTG AVINQMVNTV LITVYEKLQL VIENDSQFTC
     SLAVESKLPI KLLKDRNELF TKFINELKKT SSFKISKRDK DTLLKYFT