VTI1A_RAT
ID VTI1A_RAT Reviewed; 224 AA.
AC Q9JI51; Q9JI52;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Vesicle transport through interaction with t-SNAREs homolog 1A;
DE AltName: Full=Vesicle transport v-SNARE protein Vti1-like 2;
DE AltName: Full=Vti1-rp2;
GN Name=Vti1a; Synonyms=Vti1l2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Sprague-Dawley; TISSUE=Cerebellum, and Lung;
RX PubMed=10908612; DOI=10.1523/jneurosci.20-15-05724.2000;
RA Antonin W., Riedel D., Fischer von Mollard G.;
RT "The SNARE Vti1a-beta is localized to small synaptic vesicles and
RT participates in a novel SNARE complex.";
RL J. Neurosci. 20:5724-5732(2000).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=9705316; DOI=10.1074/jbc.273.34.21783;
RA Xu Y., Wong S.H., Tang B.L., Subramaniam V.N., Zhang T., Hong W.;
RT "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment
RT protein receptor (Vti1-rp2) implicated in protein trafficking in the
RT secretory pathway.";
RL J. Biol. Chem. 273:21783-21789(1998).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 122-199 IN COMPLEX WITH STX6;
RP STX12; VTI1A AND VAMP4, AND FUNCTION.
RX PubMed=17159904; DOI=10.1038/sj.emboj.7601467;
RA Zwilling D., Cypionka A., Pohl W.H., Fasshauer D., Walla P.J., Wahl M.C.,
RA Jahn R.;
RT "Early endosomal SNAREs form a structurally conserved SNARE complex and
RT fuse liposomes with multiple topologies.";
RL EMBO J. 26:9-18(2007).
CC -!- FUNCTION: V-SNARE that mediates vesicle transport pathways through
CC interactions with t-SNAREs on the target membrane. These interactions
CC are proposed to mediate aspects of the specificity of vesicle
CC trafficking and to promote fusion of the lipid bilayers. Involved in
CC vesicular transport from the late endosomes to the trans-Golgi network.
CC Along with VAMP7, involved in an non-conventional RAB1-dependent
CC traffic route to the cell surface used by KCNIP1 and KCND2 (By
CC similarity). May be concerned with increased secretion of cytokines
CC associated with cellular senescence. {ECO:0000250,
CC ECO:0000269|PubMed:17159904}.
CC -!- SUBUNIT: Interacts with distinct SNARE complexes that contain either
CC STX5 or STX6 (By similarity). Interacts with NAPA and, to a lesser
CC extent, with NAPG (By similarity). Identified in a complex containing
CC STX6, STX12, VAMP4 and VTI1A. {ECO:0000250,
CC ECO:0000269|PubMed:17159904}.
CC -!- INTERACTION:
CC Q9JI51; Q9R0N3: Syt11; Xeno; NbExp=2; IntAct=EBI-7573650, EBI-647443;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:9705316}; Single-
CC pass type IV membrane protein {ECO:0000269|PubMed:9705316}. Cytoplasmic
CC vesicle, secretory vesicle, synaptic vesicle membrane
CC {ECO:0000269|PubMed:9705316}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:9705316}. Cytoplasmic vesicle, clathrin-coated
CC vesicle membrane {ECO:0000269|PubMed:9705316}; Single-pass type IV
CC membrane protein {ECO:0000269|PubMed:9705316}. Golgi apparatus membrane
CC {ECO:0000269|PubMed:9705316}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:9705316}. Note=Mainly associated with the Golgi
CC apparatus. Enriched in small synaptic vesicles and clathrin-coated
CC vesicles from nerve terminals. Isoform 1/VTI1A localizes to the cell
CC bodies of hippocampal neurons as well as to nerve terminals.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2; Synonyms=VTI1A-beta;
CC IsoId=Q9JI51-1; Sequence=Displayed;
CC Name=1; Synonyms=VTI1A;
CC IsoId=Q9JI51-2; Sequence=VSP_006754;
CC -!- TISSUE SPECIFICITY: Specifically expressed in the neuronal tissues
CC cerebellum, cortex and hippocampus. Isoform 1/VTI1A is expressed in the
CC same neuronal tissues but also in lung, liver, kidney and spleen.
CC -!- SIMILARITY: Belongs to the VTI1 family. {ECO:0000305}.
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DR EMBL; AF262222; AAF97791.1; -; mRNA.
DR EMBL; AF262221; AAF97790.1; -; mRNA.
DR RefSeq; NP_075589.1; NM_023101.1. [Q9JI51-2]
DR PDB; 2NPS; X-ray; 2.50 A; C=122-199.
DR PDBsum; 2NPS; -.
DR AlphaFoldDB; Q9JI51; -.
DR SMR; Q9JI51; -.
DR BioGRID; 249335; 1.
DR CORUM; Q9JI51; -.
DR IntAct; Q9JI51; 4.
DR MINT; Q9JI51; -.
DR STRING; 10116.ENSRNOP00000058953; -.
DR PhosphoSitePlus; Q9JI51; -.
DR PaxDb; Q9JI51; -.
DR PRIDE; Q9JI51; -.
DR Ensembl; ENSRNOT00000108016; ENSRNOP00000083070; ENSRNOG00000042786. [Q9JI51-2]
DR GeneID; 65277; -.
DR KEGG; rno:65277; -.
DR UCSC; RGD:621490; rat. [Q9JI51-1]
DR CTD; 143187; -.
DR RGD; 621490; Vti1a.
DR eggNOG; KOG1666; Eukaryota.
DR GeneTree; ENSGT00950000183192; -.
DR InParanoid; Q9JI51; -.
DR OrthoDB; 1195966at2759; -.
DR PhylomeDB; Q9JI51; -.
DR Reactome; R-RNO-6811438; Intra-Golgi traffic.
DR Reactome; R-RNO-6811440; Retrograde transport at the Trans-Golgi-Network.
DR EvolutionaryTrace; Q9JI51; -.
DR PRO; PR:Q9JI51; -.
DR Proteomes; UP000002494; Chromosome 1.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005776; C:autophagosome; ISO:RGD.
DR GO; GO:0030136; C:clathrin-coated vesicle; IDA:RGD.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; IDA:ParkinsonsUK-UCL.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0044306; C:neuron projection terminus; IDA:ParkinsonsUK-UCL.
DR GO; GO:0043025; C:neuronal cell body; IDA:ParkinsonsUK-UCL.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:ParkinsonsUK-UCL.
DR GO; GO:0031201; C:SNARE complex; IDA:ParkinsonsUK-UCL.
DR GO; GO:0008021; C:synaptic vesicle; IDA:ParkinsonsUK-UCL.
DR GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043195; C:terminal bouton; HDA:ParkinsonsUK-UCL.
DR GO; GO:0005484; F:SNAP receptor activity; ISO:RGD.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; ISO:RGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:RGD.
DR GO; GO:0090161; P:Golgi ribbon formation; ISO:RGD.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0016236; P:macroautophagy; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR GO; GO:0016189; P:synaptic vesicle to endosome fusion; IDA:SynGO.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:RGD.
DR GO; GO:0050882; P:voluntary musculoskeletal movement; ISO:RGD.
DR DisProt; DP01499; -.
DR Gene3D; 1.20.58.400; -; 1.
DR InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR000727; T_SNARE_dom.
DR InterPro; IPR038407; v-SNARE_N_sf.
DR InterPro; IPR007705; Vesicle_trsprt_v-SNARE_N.
DR Pfam; PF05008; V-SNARE; 1.
DR PIRSF; PIRSF028865; Membrin-2; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Cytoplasmic vesicle;
KW Golgi apparatus; Membrane; Protein transport; Reference proteome; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..224
FT /note="Vesicle transport through interaction with t-SNAREs
FT homolog 1A"
FT /id="PRO_0000218227"
FT TOPO_DOM 1..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..224
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT COILED 31..92
FT /evidence="ECO:0000255"
FT COILED 106..185
FT /evidence="ECO:0000255"
FT VAR_SEQ 115..121
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:10908612"
FT /id="VSP_006754"
FT HELIX 124..136
FT /evidence="ECO:0007829|PDB:2NPS"
FT HELIX 138..194
FT /evidence="ECO:0007829|PDB:2NPS"
SQ SEQUENCE 224 AA; 26043 MW; 508CAC274ED055C4 CRC64;
MSADFEGYEQ DFAVLTAEIT SKISRVPRLP PDEKKQMVAN VEKQLEEARE LLEQMDLEVR
EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDAGNS SENQLIKLRE
ERAHLLDNTE RLERSSRRLE AGYQIAVETE QIGQEMLENL SHDRERIQRA RERLRETDAN
LGKSSRILTG MLRRIIQNRI LLVILGIIVV ITILTAITFF VRGH
