CALY_MOUSE
ID CALY_MOUSE Reviewed; 226 AA.
AC Q9DCA7; Q9D1K9;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Neuron-specific vesicular protein calcyon;
GN Name=Caly; Synonyms=Drd1ip;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12853145; DOI=10.1016/s0378-1119(03)00564-x;
RA Dai R., Bergson C.;
RT "Structure and expression of the murine calcyon gene.";
RL Gene 311:111-117(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=16595675; DOI=10.1074/jbc.m600265200;
RA Xiao J., Dai R., Negyessy L., Bergson C.;
RT "Calcyon, a novel partner of clathrin light chain, stimulates clathrin-
RT mediated endocytosis.";
RL J. Biol. Chem. 281:15182-15193(2006).
RN [5]
RP TRANSGENIC MICE.
RX PubMed=18295356; DOI=10.1016/j.bbr.2007.12.031;
RA Trantham-Davidson H., Vazdarjanova A., Dai R., Terry A., Bergson C.;
RT "Up-regulation of calcyon results in locomotor hyperactivity and reduced
RT anxiety in mice.";
RL Behav. Brain Res. 189:244-249(2008).
CC -!- FUNCTION: Interacts with clathrin light chain A and stimulates clathrin
CC self-assembly and clathrin-mediated endocytosis. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CLTA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}. Cell membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Most abundant in brain. Also expressed in testis
CC and ovary and, at much lower levels, in kidney and heart.
CC {ECO:0000269|PubMed:12853145}.
CC -!- DISRUPTION PHENOTYPE: Mice display impaired clathrin assembly and
CC clathrin-mediated endocytosis. Transgenic mice with up-regulated
CC forebrain expression of Caly display a range of abnormal behaviors
CC including spontaneous hyperactivity, reduced anxiety and/or impaired
CC harm avoidance. {ECO:0000269|PubMed:16595675}.
CC -!- SIMILARITY: Belongs to the NSG family. {ECO:0000305}.
CC -!- CAUTION: The human ortholog was originally thought to interact with the
CC D1 dopamine receptor (DRD1) and to play a role in potentiating calcium
CC ion-dependent signaling but this work was later retracted.
CC {ECO:0000305}.
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DR EMBL; AK002979; BAB22492.1; -; mRNA.
DR EMBL; AK003400; BAB22765.2; -; mRNA.
DR EMBL; BC049663; AAH49663.1; -; mRNA.
DR CCDS; CCDS21962.1; -.
DR RefSeq; NP_001177314.1; NM_001190385.1.
DR RefSeq; NP_001177315.1; NM_001190386.1.
DR RefSeq; NP_081045.2; NM_026769.5.
DR RefSeq; XP_006536300.1; XM_006536237.2.
DR RefSeq; XP_011248155.1; XM_011249853.2.
DR AlphaFoldDB; Q9DCA7; -.
DR BioGRID; 212931; 1.
DR STRING; 10090.ENSMUSP00000130779; -.
DR iPTMnet; Q9DCA7; -.
DR PhosphoSitePlus; Q9DCA7; -.
DR MaxQB; Q9DCA7; -.
DR PaxDb; Q9DCA7; -.
DR PRIDE; Q9DCA7; -.
DR ProteomicsDB; 265515; -.
DR Antibodypedia; 32650; 99 antibodies from 27 providers.
DR DNASU; 68566; -.
DR Ensembl; ENSMUST00000026541; ENSMUSP00000026541; ENSMUSG00000025468.
DR Ensembl; ENSMUST00000166758; ENSMUSP00000130779; ENSMUSG00000025468.
DR Ensembl; ENSMUST00000211044; ENSMUSP00000148159; ENSMUSG00000025468.
DR Ensembl; ENSMUST00000211283; ENSMUSP00000148173; ENSMUSG00000025468.
DR GeneID; 68566; -.
DR KEGG; mmu:68566; -.
DR UCSC; uc009kgr.1; mouse.
DR CTD; 50632; -.
DR MGI; MGI:1915816; Caly.
DR VEuPathDB; HostDB:ENSMUSG00000025468; -.
DR eggNOG; ENOG502QW2S; Eukaryota.
DR GeneTree; ENSGT00390000000483; -.
DR HOGENOM; CLU_112085_1_0_1; -.
DR InParanoid; Q9DCA7; -.
DR OMA; ILKQKHC; -.
DR OrthoDB; 1262422at2759; -.
DR PhylomeDB; Q9DCA7; -.
DR TreeFam; TF332232; -.
DR BioGRID-ORCS; 68566; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9DCA7; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9DCA7; protein.
DR Bgee; ENSMUSG00000025468; Expressed in dentate gyrus of hippocampal formation granule cell and 134 other tissues.
DR Genevisible; Q9DCA7; MM.
DR GO; GO:0030424; C:axon; ISO:MGI.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0098843; C:postsynaptic endocytic zone; ISO:MGI.
DR GO; GO:0032051; F:clathrin light chain binding; ISS:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0008089; P:anterograde axonal transport; ISO:MGI.
DR GO; GO:0048268; P:clathrin coat assembly; ISS:UniProtKB.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; IEA:InterPro.
DR GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR GO; GO:1905445; P:positive regulation of clathrin coat assembly; ISO:MGI.
DR GO; GO:0045807; P:positive regulation of endocytosis; ISS:UniProtKB.
DR GO; GO:2001019; P:positive regulation of retrograde axon cargo transport; ISO:MGI.
DR GO; GO:0098884; P:postsynaptic neurotransmitter receptor internalization; IDA:SynGO.
DR InterPro; IPR009431; NSG.
DR PANTHER; PTHR28546; PTHR28546; 1.
DR Pfam; PF06387; Calcyon; 1.
DR PIRSF; PIRSF002383; Calcyon; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasmic vesicle; Endocytosis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..226
FT /note="Neuron-specific vesicular protein calcyon"
FT /id="PRO_0000164369"
FT TOPO_DOM 1..88
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 177..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..217
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 226 AA; 24580 MW; AFF880F4292ADC98 CRC64;
MVKLGCSFSG KPGKEAGDQD GAAMDSVPLI SPLDVSQLQP SFSDQVVINT QTEYQLTSAD
QPKKFADLEG QRLACSHSEE GRRLPTARMI AFAMALLGCV LIMYKAIWYD QFTCPDGFLL
RHKICTPLTL EMYYTEMDPE RHRSILAAIG AYPLSRKHGT EMPAVWGNNY RTAKEEHKGT
TPAAMAVSTA AAAAAAEGTE PSGKSLDTRE KEDPQKAEGV PSQPPK
