VTM2A_HUMAN
ID VTM2A_HUMAN Reviewed; 236 AA.
AC Q8TAG5; A4D2E9; B5MC94;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=V-set and transmembrane domain-containing protein 2A {ECO:0000312|HGNC:HGNC:28499};
DE Flags: Precursor;
GN Name=VSTM2A {ECO:0000312|HGNC:HGNC:28499}; Synonyms=VSTM2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT LYS-84.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=28052263; DOI=10.1016/j.celrep.2016.12.015;
RA Secco B., Camire E., Briere M.A., Caron A., Billong A., Gelinas Y.,
RA Lemay A.M., Tharp K.M., Lee P.L., Gobeil S., Guimond J.V., Patey N.,
RA Guertin D.A., Stahl A., Haddad E., Marsolais D., Bosse Y., Birsoy K.,
RA Laplante M.;
RT "Amplification of adipogenic commitment by VSTM2A.";
RL Cell Rep. 18:93-106(2017).
CC -!- FUNCTION: Plays a role in the regulation of the early stage of white
CC and brown preadipocyte cell differentiation. Promotes adipogenic
CC commitment of preadipocytes by increasing gene expression of the
CC transcription factor PPARG in a BMP4-dependent signaling pathway.
CC {ECO:0000269|PubMed:28052263}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q8R0A6}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q8R0A6}.
CC Note=Secreted by adipose precursor cells (By similarity). Not detected
CC in the nucleus (PubMed:28052263). {ECO:0000250|UniProtKB:Q8R0A6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8TAG5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TAG5-2; Sequence=VSP_039817;
CC -!- DEVELOPMENTAL STAGE: Expressed early in fetal white adipose tissues
CC from 17 to 21 weeks of gestation (at protein level).
CC -!- PTM: N-glycosylated. N-linked glycosylation is critical for secretion
CC but not for preadipocyte cell differentiation activity.
CC {ECO:0000250|UniProtKB:Q8R0A6}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH28404.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAL23809.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC069313; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH236957; EAL23809.1; ALT_INIT; Genomic_DNA.
DR EMBL; CH471201; EAW50952.1; -; Genomic_DNA.
DR EMBL; BC028404; AAH28404.1; ALT_INIT; mRNA.
DR CCDS; CCDS5512.2; -. [Q8TAG5-1]
DR CCDS; CCDS83185.1; -. [Q8TAG5-2]
DR RefSeq; NP_001304772.1; NM_001317843.1. [Q8TAG5-2]
DR RefSeq; NP_872352.2; NM_182546.3. [Q8TAG5-1]
DR AlphaFoldDB; Q8TAG5; -.
DR SMR; Q8TAG5; -.
DR BioGRID; 128776; 6.
DR IntAct; Q8TAG5; 3.
DR MINT; Q8TAG5; -.
DR STRING; 9606.ENSP00000384103; -.
DR GlyGen; Q8TAG5; 2 sites.
DR iPTMnet; Q8TAG5; -.
DR PhosphoSitePlus; Q8TAG5; -.
DR BioMuta; VSTM2A; -.
DR DMDM; 308153542; -.
DR MassIVE; Q8TAG5; -.
DR PaxDb; Q8TAG5; -.
DR PeptideAtlas; Q8TAG5; -.
DR PRIDE; Q8TAG5; -.
DR ProteomicsDB; 73879; -. [Q8TAG5-1]
DR ProteomicsDB; 73880; -. [Q8TAG5-2]
DR Antibodypedia; 13746; 87 antibodies from 16 providers.
DR DNASU; 222008; -.
DR Ensembl; ENST00000302287.7; ENSP00000303108.3; ENSG00000170419.11. [Q8TAG5-2]
DR Ensembl; ENST00000407838.7; ENSP00000384967.3; ENSG00000170419.11. [Q8TAG5-1]
DR GeneID; 222008; -.
DR KEGG; hsa:222008; -.
DR UCSC; uc010kzf.4; human. [Q8TAG5-1]
DR CTD; 222008; -.
DR DisGeNET; 222008; -.
DR GeneCards; VSTM2A; -.
DR HGNC; HGNC:28499; VSTM2A.
DR HPA; ENSG00000170419; Tissue enhanced (adrenal gland, brain, prostate, retina).
DR neXtProt; NX_Q8TAG5; -.
DR OpenTargets; ENSG00000170419; -.
DR PharmGKB; PA162408875; -.
DR VEuPathDB; HostDB:ENSG00000170419; -.
DR eggNOG; ENOG502RKWR; Eukaryota.
DR GeneTree; ENSGT00940000160556; -.
DR InParanoid; Q8TAG5; -.
DR OMA; MWTSHDS; -.
DR OrthoDB; 1216208at2759; -.
DR PhylomeDB; Q8TAG5; -.
DR TreeFam; TF331739; -.
DR PathwayCommons; Q8TAG5; -.
DR SignaLink; Q8TAG5; -.
DR BioGRID-ORCS; 222008; 8 hits in 1060 CRISPR screens.
DR ChiTaRS; VSTM2A; human.
DR GenomeRNAi; 222008; -.
DR Pharos; Q8TAG5; Tbio.
DR PRO; PR:Q8TAG5; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q8TAG5; protein.
DR Bgee; ENSG00000170419; Expressed in middle temporal gyrus and 111 other tissues.
DR ExpressionAtlas; Q8TAG5; baseline and differential.
DR Genevisible; Q8TAG5; HS.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISS:UniProtKB.
DR GO; GO:0090336; P:positive regulation of brown fat cell differentiation; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0010884; P:positive regulation of lipid storage; IMP:UniProtKB.
DR GO; GO:0070352; P:positive regulation of white fat cell proliferation; IMP:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..236
FT /note="V-set and transmembrane domain-containing protein
FT 2A"
FT /id="PRO_0000014775"
FT DOMAIN 27..143
FT /note="Ig-like V-type"
FT REGION 184..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 48..127
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 213..236
FT /note="ARIATSHGLSVLLLVCGFVKGALL -> METHFEPFILPLTNAPQKGQSYRV
FT DRFMNGDF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_039817"
FT VARIANT 84
FT /note="E -> K (in dbSNP:rs17855529)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_047034"
SQ SEQUENCE 236 AA; 25833 MW; 3A4545C081E1C85A CRC64;
MMGIFLVYVG FVFFSVLYVQ QGLSSQAKFT EFPRNVTATE GQNVEMSCAF QSGSASVYLE
IQWWFLRGPE DLDPGAEGAG AQVELLPDRD PDSDGTKIST VKVQGNDISH KLQISKVRKK
DEGLYECRVT DANYGELQEH KAQAYLKVNA NSHARRMQAF EASPMWLQDM KPRKNVSAAI
PSSIHGSANQ RTHSTSSPQV VAKIPKQSPQ SGARIATSHG LSVLLLVCGF VKGALL
