VTS1_CRYNH
ID VTS1_CRYNH Reviewed; 687 AA.
AC J9VVN9;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=RNA-binding protein VTS1 {ECO:0000305};
GN Name=VTS1 {ECO:0000305}; Synonyms=RBP1 {ECO:0000305};
GN ORFNames=CNAG_06103 {ECO:0000312|EMBL:AFR98328.1};
OS Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN [1] {ECO:0000312|Proteomes:UP000010091}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA Cuomo C.A., Dietrich F.S.;
RT "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT grubii reveals complex RNA expression and microevolution leading to
RT virulence attenuation.";
RL PLoS Genet. 10:E1004261-E1004261(2014).
RN [2] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=27611567; DOI=10.1371/journal.ppat.1005873;
RA Park H.S., Chow E.W., Fu C., Soderblom E.J., Moseley M.A., Heitman J.,
RA Cardenas M.E.;
RT "Calcineurin Targets Involved in Stress Survival and Fungal Virulence.";
RL PLoS Pathog. 12:e1005873-e1005873(2016).
CC -!- FUNCTION: RNA-binding protein involved in post-transcriptional
CC regulation through transcript degradation.
CC {ECO:0000250|UniProtKB:Q08831}.
CC -!- SUBUNIT: Monomer. Binds to RNA. {ECO:0000250|UniProtKB:Q08831}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q08831}. Cytoplasm, P-body
CC {ECO:0000269|PubMed:27611567}. Note=Localizes to P-bodies at high
CC temperature. {ECO:0000269|PubMed:27611567}.
CC -!- SIMILARITY: Belongs to the VTS1 family. {ECO:0000305}.
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DR EMBL; CP003831; AFR98328.1; -; Genomic_DNA.
DR RefSeq; XP_012053135.1; XM_012197745.1.
DR AlphaFoldDB; J9VVN9; -.
DR SMR; J9VVN9; -.
DR EnsemblFungi; AFR98328; AFR98328; CNAG_06103.
DR GeneID; 23889341; -.
DR VEuPathDB; FungiDB:CNAG_06103; -.
DR HOGENOM; CLU_017632_1_0_1; -.
DR Proteomes; UP000010091; Chromosome 12.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; ISO:UniProtKB.
DR CDD; cd09556; SAM_VTS1_fungal; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR037635; VTS1_SAM.
DR Pfam; PF07647; SAM_2; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; RNA-binding; Translation regulation.
FT CHAIN 1..687
FT /note="RNA-binding protein VTS1"
FT /id="PRO_0000451821"
FT DOMAIN 606..667
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT REGION 1..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 248..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..598
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..74
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 278..307
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 310..325
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 545..584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 687 AA; 72547 MW; 74A69E53001089EE CRC64;
MASHTLRPHR SPSPSPEPPV NLANPAKLTR QSLGPPTSGN SKGFGSLGLG PGLASPSSPS
QPRHVSSSVA MGMGNRDSLS PRPSGVGSGR AVSATTGPRP SSEFIPTREA KTPEAEQIDQ
WFKHLANWEA TLEEMAAAST DQNFTEELGA IEQWFRVLSE AERTAALYSL LQYSTPVQIR
FFISVLHHMS QSDPMSALLS PSLSGSNAFQ TQVESKLSNM NLKSPSAGGG AGFAGSSGNG
HYLAPDDAAA KAAKARQNRI SAPGTLQPHD RWQGQLDQVV ERGTSPGLES NMSGRSRSKS
PTPEPRPKST DFSGKPRESL RRESAAFPRS PRVSAGSPGI GLGIGHESPM ASPFGNNASW
ASMVNTPLVQ NFNDGKLVAP ASVADLSQAL NMATLQLNNP GYLPLDDARK YRRPTGGLAS
GQTSRNVSGQ YNDDGEIVNP HTTQPGTPSG LLPNQFGGGR SPLVDQFGLG GLGIGADSTT
LANLGLNYNL AGLGGVNPGL NGLTAAQAQA AQMLAMQQQL QNSQYSPFNA SAPSLQPGLN
PSRRGAGRGQ SSHLNQHYNQ HQQQHQPGRR SPNPLKSHSP APGQQTGGGG AGGGAGVAGP
EDVDVKVLED VPNWLRVLRL HKYTPNFEKS NWNDMVLMTD QDLQDNGISA QGARTKFLKV
FYNVRTKMDI AHPPGQEEYA PGPKESK
