CAL_ARATH
ID CAL_ARATH Reviewed; 255 AA.
AC Q39081; C0SUX4; Q9C675; Q9S779; Q9S780; Q9SBG8; Q9SBG9; Q9SBH0; Q9SBH1;
AC Q9SBH2; Q9SBH3; Q9SBH4; Q9SBH5; Q9SBH6;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 3.
DT 25-MAY-2022, entry version 152.
DE RecName: Full=Transcription factor CAULIFLOWER;
DE Short=AtCAL;
DE AltName: Full=Agamous-like MADS-box protein AGL10;
GN Name=CAL; Synonyms=AGL10; OrderedLocusNames=At1g26310; ORFNames=F28B23.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF GLY-27; CYS-39;
RP GLU-131; 151-GLU--ALA-255 AND 176-GLU--THR-186.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=7824951; DOI=10.1126/science.7824951;
RA Kempin S.A., Savidge B., Yanofsky M.F.;
RT "Molecular basis of the cauliflower phenotype in Arabidopsis.";
RL Science 267:522-525(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-255, AND VARIANTS.
RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-0, cv. Bu-0, cv. Bu-2, cv. Ch-0,
RC cv. Co-1, cv. Columbia, cv. Firenzi, cv. Kas-0, cv. Kent,
RC cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Nd-0, cv. NL2, and
RC cv. Wassilewskija;
RX PubMed=9653152; DOI=10.1073/pnas.95.14.8130;
RA Purugganan M.D., Suddith J.I.;
RT "Molecular population genetics of the Arabidopsis CAULIFLOWER regulatory
RT gene: nonneutral evolution and naturally occurring variation in floral
RT homeotic function.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:8130-8134(1998).
RN [6]
RP FUNCTION.
RA Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R.;
RT "Control of flower development in Arabidopsis thaliana by APETALA1 and
RT interacting genes.";
RL Development 119:721-743(1993).
RN [7]
RP FUNCTION.
RX PubMed=10648231; DOI=10.1242/dev.127.4.725;
RA Ferrandiz C., Gu Q., Martienssen R., Yanofsky M.F.;
RT "Redundant regulation of meristem identity and plant architecture by
RT FRUITFULL, APETALA1 and CAULIFLOWER.";
RL Development 127:725-734(2000).
CC -!- FUNCTION: Probable transcription factor that promotes early floral
CC meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is
CC required subsequently for the transition of an inflorescence meristem
CC into a floral meristem. Seems to be partially redundant to the function
CC of APETALA1. Positively regulates the APETALA1 and LEAFY expression.
CC {ECO:0000269|PubMed:10648231, ECO:0000269|Ref.6}.
CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q39081; O64645: SOC1; NbExp=4; IntAct=EBI-592136, EBI-592041;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in young flower primordia.
CC -!- DEVELOPMENTAL STAGE: Expressed at an early stage of floral initiation.
CC -!- MISCELLANEOUS: Mutations in the CAL gene result in a characteristic
CC proliferation of inflorescence meristems in place of floral meristems.
CC The fragment expressed in cal-5 mutant is homolog to the CAL proteins
CC present in Brassica oleracea var. botrytis and may thus explain the
CC 'cauliflower'-shaped floral meristem (PubMed:7824951).
CC {ECO:0000305|PubMed:7824951}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA64789.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67505.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67506.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67507.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67508.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67509.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67510.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67511.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67512.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67513.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67514.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67515.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67516.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67517.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67518.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC67519.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG50679.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; L36925; AAA64789.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC079829; AAG50679.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30674.1; -; Genomic_DNA.
DR EMBL; AB493477; BAH30315.1; -; mRNA.
DR EMBL; AF061401; AAC67505.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061402; AAC67506.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061403; AAC67507.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061404; AAC67508.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061405; AAC67509.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061406; AAC67510.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061407; AAC67511.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061408; AAC67512.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061409; AAC67513.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061410; AAC67514.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061411; AAC67515.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061412; AAC67516.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061413; AAC67517.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061414; AAC67518.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF061415; AAC67519.1; ALT_SEQ; Genomic_DNA.
DR PIR; E86389; E86389.
DR RefSeq; NP_564243.1; NM_102395.3.
DR AlphaFoldDB; Q39081; -.
DR SMR; Q39081; -.
DR BioGRID; 24408; 5.
DR DIP; DIP-33764N; -.
DR IntAct; Q39081; 6.
DR STRING; 3702.AT1G26310.1; -.
DR PaxDb; Q39081; -.
DR PRIDE; Q39081; -.
DR EnsemblPlants; AT1G26310.1; AT1G26310.1; AT1G26310.
DR GeneID; 839172; -.
DR Gramene; AT1G26310.1; AT1G26310.1; AT1G26310.
DR KEGG; ath:AT1G26310; -.
DR Araport; AT1G26310; -.
DR TAIR; locus:2028830; AT1G26310.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; Q39081; -.
DR OMA; EDQTAMR; -.
DR OrthoDB; 1178730at2759; -.
DR PhylomeDB; Q39081; -.
DR PRO; PR:Q39081; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39081; baseline and differential.
DR Genevisible; Q39081; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010582; P:floral meristem determinacy; IEP:TAIR.
DR GO; GO:0009911; P:positive regulation of flower development; TAS:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Developmental protein; Differentiation;
KW DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..255
FT /note="Transcription factor CAULIFLOWER"
FT /id="PRO_0000199480"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 90..180
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT COILED 90..198
FT /evidence="ECO:0000255"
FT VARIANT 78
FT /note="R -> S (in strain: cv. Kas-0)"
FT VARIANT 114
FT /note="Y -> H (in strain: cv. Bu-0)"
FT VARIANT 120
FT /note="E -> G (in strain: cv. Bretagny)"
FT VARIANT 122
FT /note="M -> K (in strain: cv. Kas-0)"
FT VARIANT 141
FT /note="I -> T (in strain: cv. Bu-0)"
FT VARIANT 161
FT /note="K -> E (in strain: cv. Kent)"
FT VARIANT 176
FT /note="E -> K (in strain: cv. Kent and cv. Wassilewskija)"
FT VARIANT 182
FT /note="R -> K (in strain: cv. Bu-0, cv. Landsberg erecta,
FT cv. Li-8 and cv. NL2)"
FT VARIANT 184
FT /note="K -> R (in strain: cv. NL2)"
FT VARIANT 186
FT /note="T -> I (in strain: cv. Wassilewskija)"
FT VARIANT 195
FT /note="V -> I (in strain: cv. Co-1)"
FT VARIANT 196
FT /note="D -> N (in strain: cv. Kent)"
FT VARIANT 205
FT /note="Q -> QL (in strain: cv. Bu-2)"
FT VARIANT 209
FT /note="L -> F (in strain: cv. Li-3)"
FT VARIANT 228
FT /note="E -> G (in strain: cv. Bla-1, cv. Bretagny, cv. Bs-
FT 0, cv. Bu-0, cv. Bu-2, cv. Co-1, cv. Kas-0, cv. Kent, cv.
FT Landsberg erecta, cv. Li-3, cv. Li-8, cv. Nd-0, cv. NL2 and
FT cv. Wassilewskija)"
FT VARIANT 238
FT /note="N -> D (in strain: cv. Bu-2)"
FT VARIANT 249
FT /note="Y -> C (in strain: cv. NL2)"
FT MUTAGEN 27
FT /note="G->D: In cal-3; no visible phenotype. 'Cauliflower'
FT shape when associated with AP1 disruption."
FT /evidence="ECO:0000269|PubMed:7824951"
FT MUTAGEN 39
FT /note="C->Y: In cal-2; no visible phenotype. 'Cauliflower'
FT shape when associated with AP1 disruption."
FT /evidence="ECO:0000269|PubMed:7824951"
FT MUTAGEN 131
FT /note="E->K: In cal-4; no visible phenotype. 'Cauliflower'
FT shape when associated with AP1 disruption."
FT /evidence="ECO:0000269|PubMed:7824951"
FT MUTAGEN 151..255
FT /note="Missing: In cal-5; 'Cauliflower' shape."
FT /evidence="ECO:0000269|PubMed:7824951"
FT MUTAGEN 176..186
FT /note="ERENILRTKQT->KRENILRTRQI: In cal-1; no visible
FT phenotype. 'Cauliflower' shape when associated with AP1
FT disruption."
FT /evidence="ECO:0000269|PubMed:7824951"
SQ SEQUENCE 255 AA; 30187 MW; 5F7313A804DF594D CRC64;
MGRGRVELKR IENKINRQVT FSKRRTGLLK KAQEISVLCD AEVSLIVFSH KGKLFEYSSE
SCMEKVLERY ERYSYAERQL IAPDSHVNAQ TNWSMEYSRL KAKIELLERN QRHYLGEELE
PMSLKDLQNL EQQLETALKH IRSRKNQLMN ESLNHLQRKE KEIQEENSML TKQIKERENI
LRTKQTQCEQ LNRSVDDVPQ PQPFQHPHLY MIAHQTSPFL NMGGLYQEED QTAMRRNNLD
LTLEPIYNYL GCYAA
