ID   VVHA_VIBVU              Reviewed;         471 AA.
AC   P19247;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 138.
DE   RecName: Full=Cytolysin;
DE   Flags: Precursor;
GN   Name=vvhA; OrderedLocusNames=VV2_0404;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2370116; DOI=10.1128/iai.58.8.2706-2709.1990;
RA   Yamamoto K., Wright A.C., Kaper J.B., Morris J.G. Jr.;
RT   "The cytolysin gene of Vibrio vulnificus: sequence and relationship to the
RT   Vibrio cholerae E1 Tor hemolysin gene.";
RL   Infect. Immun. 58:2706-2709(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC       membranes and cause cell rupture by mechanisms not clearly defined.
CC   -!- SIMILARITY: Belongs to the HlyA hemolysin family. {ECO:0000305}.
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DR   EMBL; M34670; AAA03245.1; -; Unassigned_DNA.
DR   EMBL; AE016796; AAO07360.2; -; Genomic_DNA.
DR   PIR; A41478; A41478.
DR   RefSeq; WP_011081361.1; NC_004460.2.
DR   PDB; 4OWJ; X-ray; 2.00 A; A/B/C/D/E/F/G=338-471.
DR   PDB; 4OWK; X-ray; 2.00 A; A/B/C/D/E/F/G=338-471.
DR   PDB; 4OWL; X-ray; 2.10 A; A/B/C/D/E/F/G=338-471.
DR   PDBsum; 4OWJ; -.
DR   PDBsum; 4OWK; -.
DR   PDBsum; 4OWL; -.
DR   AlphaFoldDB; P19247; -.
DR   SMR; P19247; -.
DR   CAZy; CBM13; Carbohydrate-Binding Module Family 13.
DR   TCDB; 1.C.14.1.3; the cytohemolysin (chl) family.
DR   UniLectin; P19247; -.
DR   PRIDE; P19247; -.
DR   EnsemblBacteria; AAO07360; AAO07360; VV2_0404.
DR   KEGG; vvu:VV2_0404; -.
DR   HOGENOM; CLU_579932_0_0_6; -.
DR   OMA; HVAFYLN; -.
DR   PHI-base; PHI:6877; -.
DR   PHI-base; PHI:8497; -.
DR   Proteomes; UP000002275; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   CDD; cd00161; RICIN; 1.
DR   InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR   InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   InterPro; IPR000772; Ricin_B_lectin.
DR   Pfam; PF07968; Leukocidin; 1.
DR   Pfam; PF00652; Ricin_B_lectin; 1.
DR   SMART; SM00458; RICIN; 1.
DR   SUPFAM; SSF50370; SSF50370; 1.
DR   SUPFAM; SSF56959; SSF56959; 1.
DR   PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytolysis; Hemolysis; Lectin; Signal; Toxin; Virulence.
FT   SIGNAL          1..20
FT   CHAIN           21..471
FT                   /note="Cytolysin"
FT                   /id="PRO_0000013365"
FT   DOMAIN          338..465
FT                   /note="Ricin B-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00174"
FT   CONFLICT        13
FT                   /note="A -> R (in Ref. 1; AAA03245)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        338
FT                   /note="A -> R (in Ref. 1; AAA03245)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        435
FT                   /note="N -> S (in Ref. 1; AAA03245)"
FT                   /evidence="ECO:0000305"
FT   STRAND          339..347
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          351..357
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          360..363
FT                   /evidence="ECO:0007829|PDB:4OWL"
FT   STRAND          367..371
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   HELIX           377..379
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          380..383
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          389..391
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          394..396
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          398..401
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          407..410
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   HELIX           416..418
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          420..423
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          426..429
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          439..445
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          448..453
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   HELIX           454..456
FT                   /evidence="ECO:0007829|PDB:4OWJ"
FT   STRAND          462..466
FT                   /evidence="ECO:0007829|PDB:4OWJ"
SQ   SEQUENCE   471 AA;  52899 MW;  F5F41B504552BAFA CRC64;
     MKKMTLFTLS LLATAVQVGA QEYVPIVEKP IYITSSKIKC VLHTSGDFNA TRDWCNAGAS
     IDVRVNVAQM RSVQSATSDG FTPDAKIVRF TVDADKPGTG IHLVNELQQD HSWFQSWANR
     RTYIGPFASS YDLWVKPVSG YTPKKARDLP QNENKNYQHR DTYGYSIGIN GKVGAEVNKD
     GPKVGGEVSG SFTYNYSKTL VFDTKDYRIN NRSSLSDFDI SFEREFGECD ELRRQELGCY
     FTAAHWGSGW VFDKTKFNPI SYSNFKPNYD VLYEAPVSET GVTDFEMGVK LNYRARFGTV
     LPSALFSVYG SAGSSTNSST VKQRIRIDWN HPLFEAEAHV TLQSLSNNDL CLDVYGENGD
     KTVAGGSVNG WSCHGSWNQV WGLDKEERYR SRVASDRCLT VNADKTLTVE QCGANLAQKW
     YWEGDKLISR YVDGNNTRYL LNIVGGRNVQ VTPENEANQA RWKPTLQQVK L