VWCE_HUMAN
ID VWCE_HUMAN Reviewed; 955 AA.
AC Q96DN2; A5PKV0; Q7Z7L6; Q86WK8;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=von Willebrand factor C and EGF domain-containing protein;
DE AltName: Full=HBV X protein up-regulated gene 11 protein;
DE AltName: Full=HBxAg up-regulated gene 11 protein;
DE Flags: Precursor;
GN Name=VWCE; Synonyms=URG11;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 73-955 (ISOFORM 1).
RA Lian Z., Liu J., Li L., Li X., Tufan S.N.L., Clayton M., Wu M.-C.,
RA Wang H.-Y., Arbuthnot P., Kew M., Feitelson M.A.;
RT "Upregulated expression of a unique gene by hepatitis B x antigen promotes
RT hepatocellular growth and tumorigenesis.";
RL Neoplasia 5:229-244(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HBXAG, AND TISSUE SPECIFICITY.
RX PubMed=16496348; DOI=10.1002/hep.21053;
RA Lian Z., Liu J., Li L., Li X., Clayton M., Wu M.-C., Wang H.-Y.,
RA Arbuthnot P., Kew M., Fan D., Feitelson M.A.;
RT "Enhanced cell survival of Hep3B cells by the hepatitis B x antigen
RT effector, URG11, is associated with upregulation of beta-catenin.";
RL Hepatology 43:415-424(2006).
CC -!- FUNCTION: May be a regulatory element in the beta-catenin signaling
CC pathway and a target for chemoprevention of hapatocellular carcinoma.
CC {ECO:0000269|PubMed:16496348}.
CC -!- INTERACTION:
CC Q96DN2; Q3LI76: KRTAP15-1; NbExp=3; IntAct=EBI-2850497, EBI-11992140;
CC Q96DN2; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-2850497, EBI-16439278;
CC Q96DN2; P17024: ZNF20; NbExp=3; IntAct=EBI-2850497, EBI-717634;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Note=According to
CC PubMed:16496348 is localized in the cytoplasm.
CC {ECO:0000269|PubMed:16496348}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96DN2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96DN2-2; Sequence=VSP_031230, VSP_031231;
CC -!- TISSUE SPECIFICITY: Expressed in liver. {ECO:0000269|PubMed:16496348}.
CC -!- INDUCTION: By HBxAg. {ECO:0000269|PubMed:16496348}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO47606.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK056571; BAB71219.1; -; mRNA.
DR EMBL; BC052564; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC077719; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC142624; AAI42625.1; -; mRNA.
DR EMBL; CH471076; EAW73932.1; -; Genomic_DNA.
DR EMBL; AY205235; AAO47606.1; ALT_FRAME; mRNA.
DR CCDS; CCDS8002.1; -. [Q96DN2-1]
DR RefSeq; NP_689931.2; NM_152718.2. [Q96DN2-1]
DR AlphaFoldDB; Q96DN2; -.
DR BioGRID; 128616; 238.
DR IntAct; Q96DN2; 61.
DR MINT; Q96DN2; -.
DR STRING; 9606.ENSP00000334186; -.
DR GlyGen; Q96DN2; 7 sites, 2 O-linked glycans (5 sites).
DR iPTMnet; Q96DN2; -.
DR PhosphoSitePlus; Q96DN2; -.
DR BioMuta; VWCE; -.
DR DMDM; 269849477; -.
DR MassIVE; Q96DN2; -.
DR PaxDb; Q96DN2; -.
DR PeptideAtlas; Q96DN2; -.
DR PRIDE; Q96DN2; -.
DR ProteomicsDB; 76297; -. [Q96DN2-1]
DR Antibodypedia; 52915; 108 antibodies from 17 providers.
DR DNASU; 220001; -.
DR Ensembl; ENST00000301770.10; ENSP00000301770.6; ENSG00000167992.14. [Q96DN2-2]
DR Ensembl; ENST00000335613.10; ENSP00000334186.5; ENSG00000167992.14. [Q96DN2-1]
DR GeneID; 220001; -.
DR KEGG; hsa:220001; -.
DR MANE-Select; ENST00000335613.10; ENSP00000334186.5; NM_152718.2; NP_689931.2.
DR UCSC; uc001nra.4; human. [Q96DN2-1]
DR CTD; 220001; -.
DR DisGeNET; 220001; -.
DR GeneCards; VWCE; -.
DR HGNC; HGNC:26487; VWCE.
DR HPA; ENSG00000167992; Tissue enhanced (liver, ovary).
DR MIM; 611115; gene.
DR neXtProt; NX_Q96DN2; -.
DR OpenTargets; ENSG00000167992; -.
DR PharmGKB; PA143485669; -.
DR VEuPathDB; HostDB:ENSG00000167992; -.
DR eggNOG; KOG1216; Eukaryota.
DR eggNOG; KOG1217; Eukaryota.
DR GeneTree; ENSGT00940000161089; -.
DR HOGENOM; CLU_1261118_0_0_1; -.
DR InParanoid; Q96DN2; -.
DR OMA; PLCTGCF; -.
DR OrthoDB; 1223914at2759; -.
DR PhylomeDB; Q96DN2; -.
DR TreeFam; TF330819; -.
DR PathwayCommons; Q96DN2; -.
DR SignaLink; Q96DN2; -.
DR BioGRID-ORCS; 220001; 19 hits in 1073 CRISPR screens.
DR ChiTaRS; VWCE; human.
DR GenomeRNAi; 220001; -.
DR Pharos; Q96DN2; Tbio.
DR PRO; PR:Q96DN2; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96DN2; protein.
DR Bgee; ENSG00000167992; Expressed in right uterine tube and 117 other tissues.
DR ExpressionAtlas; Q96DN2; baseline and differential.
DR Genevisible; Q96DN2; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0098586; P:cellular response to virus; IEP:UniProtKB.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR InterPro; IPR001007; VWF_dom.
DR Pfam; PF07645; EGF_CA; 3.
DR Pfam; PF00093; VWC; 4.
DR SMART; SM00181; EGF; 4.
DR SMART; SM00179; EGF_CA; 3.
DR SMART; SM00214; VWC; 6.
DR SMART; SM00215; VWC_out; 3.
DR SUPFAM; SSF57184; SSF57184; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 3.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS01186; EGF_2; 2.
DR PROSITE; PS50026; EGF_3; 3.
DR PROSITE; PS01187; EGF_CA; 3.
DR PROSITE; PS01208; VWFC_1; 5.
DR PROSITE; PS50184; VWFC_2; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Disulfide bond; EGF-like domain;
KW Glycoprotein; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..955
FT /note="von Willebrand factor C and EGF domain-containing
FT protein"
FT /id="PRO_0000318580"
FT DOMAIN 70..98
FT /note="EGF-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 142..180
FT /note="EGF-like 2; calcium-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 181..219
FT /note="EGF-like 3; calcium-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 220..262
FT /note="EGF-like 4; calcium-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 384..441
FT /note="VWFC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 442..502
FT /note="VWFC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 503..560
FT /note="VWFC 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 566..626
FT /note="VWFC 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 627..685
FT /note="VWFC 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 686..743
FT /note="VWFC 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT REGION 292..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..889
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 903..955
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 841..872
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 918..937
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 462
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 472
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 146..155
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 151..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 166..179
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 185..194
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 190..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 205..218
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 224..237
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 233..246
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 248..261
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT VAR_SEQ 219
FT /note="V -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031230"
FT VAR_SEQ 220..955
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031231"
FT VARIANT 842
FT /note="P -> R (in dbSNP:rs3750982)"
FT /id="VAR_038782"
FT CONFLICT 134
FT /note="T -> A (in Ref. 1; BAB71219)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 955 AA; 99915 MW; B745618AD37E712B CRC64;
MWAGLLLRAA CVALLLPGAP ARGYTGRKPP GHFAAERRRL GPHVCLSGFG SGCCPGWAPS
MGGGHCTLPL CSFGCGSGIC IAPNVCSCQD GEQGATCPET HGPCGEYGCD LTCNHGGCQE
VARVCPVGFS MTETAVGIRC TDIDECVTSS CEGHCVNTEG GFVCECGPGM QLSADRHSCQ
DTDECLGTPC QQRCKNSIGS YKCSCRTGFH LHGNRHSCVD VNECRRPLER RVCHHSCHNT
VGSFLCTCRP GFRLRADRVS CEAFPKAVLA PSAILQPRQH PSKMLLLLPE AGRPALSPGH
SPPSGAPGPP AGVRTTRLPS PTPRLPTSSP SAPVWLLSTL LATPVPTASL LGNLRPPSLL
QGEVMGTPSS PRGPESPRLA AGPSPCWHLG AMHESRSRWT EPGCSQCWCE DGKVTCEKVR
CEAACSHPIP SRDGGCCPSC TGCFHSGVVR AEGDVFSPPN ENCTVCVCLA GNVSCISPEC
PSGPCQTPPQ TDCCTCVPVR CYFHGRWYAD GAVFSGGGDE CTTCVCQNGE VECSFMPCPE
LACPREEWRL GPGQCCFTCQ EPTPSTGCSL DDNGVEFPIG QIWSPGDPCE LCICQADGSV
SCKRTDCVDS CPHPIRIPGQ CCPDCSAGCT YTGRIFYNNE TFPSVLDPCL SCICLLGSVA
CSPVDCPITC TYPFHPDGEC CPVCRDCNYE GRKVANGQVF TLDDEPCTRC TCQLGEVSCE
KVPCQRACAD PALLPGDCCS SCPDSLSPLE EKQGLSPHGN VAFSKAGRSL HGDTEAPVNC
SSCPGPPTAS PSRPVLHLLQ LLLRTNLMKT QTLPTSPAGA HGPHSLALGL TATFPGEPGA
SPRLSPGPST PPGAPTLPLA SPGAPQPPPV TPERSFSASG AQIVSRWPPL PGTLLTEASA
LSMMDPSPSK TPITLLGPRV LSPTTSRLST ALAATTHPGP QQPPVGASRG EESTM
