W4932_FUSPC
ID W4932_FUSPC Reviewed; 8892 AA.
AC K3VDP2;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 23-FEB-2022, entry version 44.
DE RecName: Full=Nonribosomal peptide synthetase 32 {ECO:0000303|PubMed:25412204};
DE Short=NRPS 32 {ECO:0000303|PubMed:25412204};
DE EC=6.3.2.- {ECO:0000305|PubMed:25412204};
DE AltName: Full=W493 A and B biosynthesis cluster protein NRPS320 {ECO:0000303|PubMed:25412204};
GN Name=NRPS32 {ECO:0000303|PubMed:25412204}; ORFNames=FPSE_09183;
OS Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot
OS fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=1028729;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CS3096;
RX PubMed=23028337; DOI=10.1371/journal.ppat.1002952;
RA Gardiner D.M., McDonald M.C., Covarelli L., Solomon P.S., Rusu A.G.,
RA Marshall M., Kazan K., Chakraborty S., McDonald B.A., Manners J.M.;
RT "Comparative pathogenomics reveals horizontally acquired novel virulence
RT genes in fungi infecting cereal hosts.";
RL PLoS Pathog. 8:E1002952-E1002952(2012).
RN [2]
RP IDENTIFICATION, FUNCTION, AND PATHWAY.
RX PubMed=25412204; DOI=10.1021/np500436r;
RA Soerensen J.L., Sondergaard T.E., Covarelli L., Fuertes P.R., Hansen F.T.,
RA Frandsen R.J., Saei W., Lukassen M.B., Wimmer R., Nielsen K.F.,
RA Gardiner D.M., Giese H.;
RT "Identification of the biosynthetic gene clusters for the lipopeptides
RT fusaristatin A and W493 B in Fusarium graminearum and F.
RT pseudograminearum.";
RL J. Nat. Prod. 77:2619-2625(2014).
CC -!- FUNCTION: Nonribosomal peptide synthetase; part of the gene cluster
CC that mediates the biosynthesis of the lipopeptides W493 A and B
CC (PubMed:25412204). W493 A and B consist of six amino acid residues D-
CC allo-thr, L-Ala, D-Ala, L-Gln, D-Tyr, and L-Val/L-Ile linked to a 3-
CC hydroxy-4-methyltetradecanoic acid polyketide chain (PubMed:25412204).
CC The biosynthesis starts with formation of the linear polyketide chain
CC by the highly reducing polyketide synthase PKS40 (PubMed:25412204). The
CC gene cluster contains a putative acyl-CoA ligase (FPSE_09184) for
CC formation of a CoA thioester polyketide. The thiol bond could be
CC hydrolyzed by the putative thioesterase (FPSE_09186) and then accepted
CC by the first T domain in module 1 of NRPS32 (PubMed:25412204). The
CC second T domain is responsible for accepting a threonine, which is
CC adenylated by the A domain and epimerized to the D-allo-threonine
CC formed by the E domain. The five successive modules incorporate Ala,
CC Ala, Gln, Tyr, and Val/Ile into the final product, which is released by
CC cyclization (PubMed:25412204). {ECO:0000269|PubMed:25412204}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:25412204}.
CC -!- DOMAIN: NRP synthetases are composed of discrete domains (adenylation
CC (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation
CC (C) domains) which when grouped together are referred to as a single
CC module. Each module is responsible for the recognition (via the A
CC domain) and incorporation of a single amino acid into the growing
CC peptide product. Thus, an NRP synthetase is generally composed of one
CC or more modules and can terminate in a thioesterase domain (TE) that
CC releases the newly synthesized peptide from the enzyme. Occasionally,
CC epimerase (E) domains (responsible for L- to D-amino acid conversion)
CC are present within the NRP synthetase (By similarity). NRPS32 has the
CC following architecture: T-C-A-T-E-C-A-T-E-C-A-T-C-A-T-C-A-T-E-C-A-T-Cy.
CC The last condensation domain (Cy) could be responsible for cyclization
CC of the final product (Probable). {ECO:0000250|UniProtKB:Q4WAZ9,
CC ECO:0000305|PubMed:25412204}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR EMBL; AFNW01000306; EKJ70673.1; -; Genomic_DNA.
DR RefSeq; XP_009260575.1; XM_009262300.1.
DR STRING; 101028.EKJ70673; -.
DR EnsemblFungi; EKJ70673; EKJ70673; FPSE_09183.
DR GeneID; 20367800; -.
DR KEGG; fpu:FPSE_09183; -.
DR eggNOG; KOG1176; Eukaryota.
DR eggNOG; KOG1178; Eukaryota.
DR HOGENOM; CLU_222660_0_0_1; -.
DR InParanoid; K3VDP2; -.
DR Proteomes; UP000007978; Chromosome 1.
DR Proteomes; UP000007978; Unassembled WGS sequence.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR Gene3D; 1.10.1200.10; -; 7.
DR Gene3D; 3.30.300.30; -; 6.
DR Gene3D; 3.30.559.10; -; 11.
DR Gene3D; 3.40.50.12780; -; 5.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF00668; Condensation; 10.
DR Pfam; PF00550; PP-binding; 7.
DR SMART; SM00823; PKS_PP; 7.
DR SUPFAM; SSF47336; SSF47336; 7.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 6.
DR PROSITE; PS00455; AMP_BINDING; 5.
DR PROSITE; PS50075; CARRIER; 7.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 3: Inferred from homology;
KW Isomerase; Ligase; Phosphopantetheine; Phosphoprotein; Repeat.
FT CHAIN 1..8892
FT /note="Nonribosomal peptide synthetase 32"
FT /id="PRO_0000445375"
FT DOMAIN 12..85
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 1523..1599
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3061..3137
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4627..4703
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 5745..5821
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 6834..6911
FT /note="Carrier 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 8380..8456
FT /note="Carrier 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 88..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 567..956
FT /note="Condensation 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 989..1386
FT /note="Adenylation 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 1609..2039
FT /note="Epimerization 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 2083..2518
FT /note="Condensation 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 2543..2934
FT /note="Adenylation 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 3153..3590
FT /note="Epimerization 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 3634..4061
FT /note="Condensation 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 4098..4488
FT /note="Adenylation 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 4760..5181
FT /note="Condensation 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 5205..5605
FT /note="Adenylation 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 5868..6285
FT /note="Condensation 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 6307..6700
FT /note="Adenylation 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 6923..7360
FT /note="Epimerization 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 7403..7834
FT /note="Condensation 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 7855..8253
FT /note="Adenylation 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT REGION 8490..8878
FT /note="Condensation 7"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25412204"
FT MOD_RES 46
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 1560
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3098
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4664
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 5782
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 6872
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 8417
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 8892 AA; 976639 MW; D576AC167EB80D6F CRC64;
MENPNNGQAH FEPSKLVLGR VAADLFDTDV DSLDWGKSFI MLGGDSILAI DFIVRCRDAG
ILVDMRDLLT AGTLAQLAES IAQQNTGAGN GVNGHANGNG MNGNGLHNEA TIRNGSDDLT
TSAAAAGRRL RFATMEIPEN TDASIISTAM ERIVDRHSAL RSNWSISSTG KWIITTASGS
GLKSRQFFYG EFAEATEVTD AFEWLRKALL SDELFPSGCL VIPSDAPGCS HTIVLAADAN
VVDALSMGLV LRELRETING TQLELSSDFQ FSDWIARGCQ GLEAQVERAN TTKSNSITDT
GALKLNGNTR DNIDFQLSRL ATERLFAAQT HAALRTSPVD IVNAAVSQSL GPRYRDTENV
LTIISAYSIR EEKDIPLDSI GCYEVETELA ATAPVPGETL VHLVRQMRDA RAGFSADSRS
STAIYVDCTR LCHFEEGDYS PWLSDPTPDH SPYVSLAMIA GQVHVSLHFG IARPDDKLIA
DRLRSCLDEI VDELAKAPRM GTLHDFPLVR WSYSALDGLA RDLQTHGLDF GDIESIGPST
SVQESFFVSQ AINPGSYVSH ATLRLLPTSA NVPRRVETEK VVRAWSDIVE RHAMLRTAFA
ESNDRPGKFD QLVFKPTALP PRVIVISSSA ETSIVSPFTA GRFESPLRLC IYEINAEELR
LELEISHALV DGHSAKILLH DIRASYLQSA YFSQSAPLPY TVFASRQQAT LSEGEASAGI
TYWTSYLNEA SESHLPLITT NPQLKDLETT RCSVSVPDGK LRAVCGELSI TPANLFHVAW
ALALRRIILS DRITFSYIVS GRNSDADGVE ATVGPFVNTL PFALALNPEN SIADVLGSAK
QDWQNGLQFQ NIPIADLAAA KTRSLKRLGN TLLSIEREGS NTHPFTEGTY MSLDARTSAA
DFDLIANIRF NEQRINLSVE YWASRIAGPV AKAQMAAFED AVSFLLEGVD LSVRDFPSHS
PQDRAAFIRW NSTKPARLES CVHELVVEKM AEQPEALAVS SWDGELTYGE LERASRRLAY
HLVEKGVGPE VMVGMCMEKS KLGVVAMVAT LLAGGGLVPL GVSHPLARIE GIVKDAASPL
ILVDRVHEER LAELGSYTEL VAVDSFFDNA SSTAIPSSGP FTSVGPDNVA WMIFTSGSTG
KPKGVVLEHG SLATSILYHG RRLDIQPHDR LLQFAAFTFD AAIQEIITAL AFGATTCIPS
ESDRMDRLSN YLAESKVTIA TLTSTVAALV RPQETPTVRT IILMGEAVQA KVVDQWIDYA
TVINAYGPSE CCIHSTCRPV TDSSASLNIG TAIAGATWVV NPTNINQLVP LGGQGELLLE
GPLLARGYLN DSVKTAQAFV TDPAFVRELE LGSNQHRMYR TGDLVQQNTD GTLTYLGRID
SQIKIRGQRV EIGEIEYHIG KQGGVHDAVV LYMRQGPLAD RLVAAVNLGE SSSVDRPQTS
TVQVVSEDHM ANARLRLREV QHGLSRQVMH YMVPSIWLPL SAMPMNQSGK TDRRALTDWI
KSLSSDDIAA LSNGGDADDV DDISATAVER ELRQIWSEVL AVPLGSVTYS SSFFSLGGDS
ITAMQVVSAS RSRGILITVR KVLDCQTIPE LAAEVQATQN SDYAAVPEGA IALSPIQQMY
FESIAADGLR ADNEHCFNQG VLLHVTRQIT LAELTRALDM AVAKHAMLRA RFQYSQEQGW
QQRIEREVTG SYRLCAHTAG DTEMSGIVAQ SQSTLDIENG PVFAADLIER EDRQVLHLVA
HHLVIDLVSW RILLRDLEEV IVNQKLPNPS SLSFPTWLGR QKESLSKVSN VLDTLPMIVP
KTNWTYWGLT PGQETFGSRS STQARCDIET TSLLTGGANS PLKTEPVEIL LAGLLLSFQQ
VFADRPVPTV FTEGHGREAP DDGTDLSETI GWFTTMSPIY VSFTEASSNG AIDVLRQVKD
QRRRIPGRGV PYFGSRFLTA QGKEQFAGHG PAEIMFNYTG RFQQLERDDA LFHFDRDNNS
STMARAGDLV KLFAALDVAI TIEAGKLCIT VHYSNQSRCQ EGIRRWVEVY GQTVKSLVNA
LNTVEPMATA TDFPLARLSD SDMETIEGQY LPMIGLTSSA EIEDILPCSP IQQGILLTQL
QSPSTYCIYQ TCRIVPSGDA PVSADRLIAA WREVVSRHSI LRTVLLEPLQ GQENFIQVVL
RHPEIGIIKK DGITDGVTAA EWLASRPSLD VSILGRPPHL LTVLTTVSGE VYCRFDISHA
LVDASSVTLI MRDLMDAYDE EKSQSAVSGS KYSTYVAFLQ GRDSQDDLQY WTSLLQDAEP
CLLPPNDPTH DAGDQPTIKK VSTRIDDIGK LHQFRDKYGV SVASIFQLSW ALVLAMQTCS
RNVSFGNLSS GRDVPISGVQ ELVGPMINML VCHLDLNWGE GVSEAARKLQ SQSTEAFEHQ
RTSLAAIQHG LGLSRNQPLF NSTLSYKRQA STSSGRASSI TLEGLTWEDP TEYDVHINIE
ASSTSIDINM QYSTAAFSET TTKKLVDSLV HAVFAVCGGG DRALGQLRLL LPAEEAKLCE
WNSVIPPRVE RCVHELVLDR MVSQPEALAV SAWDGDLTYA ELNHSSYQLA HHLVAERGIG
PEVMVGLCMD KSKWAIVAMI AVLRAGGTLV PLGVQEPFSR IEMITGETGT PLVLVDRHHE
QRVAGLDTQL FTVDYFFDAV SPISMLPPFA ELSATRPDHA AWVIFTSGST GRPKGVVLEH
GSVATSILAH GPAIGIQIQD RVSQFAAYTF DVSIAEIMTT LTFGACVCVP SEDDRTNRLT
QFLSESEVSI ATLTSTVAAL VQPDKTPKIR TLVLTGEAVQ PKVVDQWMHR ATVINAYGPA
ESSIWTTGKV IENALDATNI GTPLAGAFWV VNPDSVGQLV HIGELLIESP LLARGYLNDP
VKTAASFITN LELLKNLGLG IGSRRMYRTG DLVRQNQDGT ITYLGRRDTQ VKIRGMQDAV
VLYMNQGALA GRLIAFIVSS DTLAASHDQS KPQSSASIQR VHEEQNETAD IWLKDVQQNL
SQLVMHYMMP SVWIRLLAIP INASGKTDRL GLFRWVESLS AEDTAALTDA GAIEGEDIDE
SSATPIEREL RQIWSEVLDV PISRVTYSTN FFSLGGDSIT AMQVVSACRA RGILVTVRKV
LDCQTIPELA ANSQTGREDA AHLARIPEGV FGLSPIQQMF FDEIAGDGLR ADMDYRFNQA
VSLYTTQRIE KTDLIWAINA LVSKHAMLRA RFRYTQDNAW QQWIEKDITG SYRFQDHTVA
DVELMQNLIE KSQATLDLEH GPVFAIDFIE RQDRQGRQVL HLVAHHLVID LVSWRILIRD
LEELLVHHKL PNPTSLSFPV WLERQQDSLN RLVTETEEGA DKSLEETLPV VVPNANWGYW
GLVPGNDIYA SLSTIEVQCG SATTSLLSGN ANNALKTEPV DILLAALISS FQDVFADRSM
PAVFTEGHGR ETEEEEIDLS DTVSWFTTMV PVHVPQGAAK NAIEVLRKVK DQRRRLPGKG
LPYFSSRYLT SHGREKFASH GPAEIIFNYL GRFQQLERDD ALFHIEEDDT SASSQFGPLV
KLFSVLDVSA GVEAGQLSIK VRFSRESLHQ SAIKQWVKLY GDTVKTLVEE LTMVPLTLTA
TDFPLARLSD SDWELIEGQY LKTEMGLSST KEVEDILPCS AIQQGILLTQ LQSPSTYCIH
QTCRILPTDH TRPVSIQRLV VAWHQVVARH SILRSVLVEP LPAQESFLQI VLREPQIDVQ
ITDGEDIRDE EAVEWLASQS TLDITKLSRP PHRLIILKTN TDNVYCRFDV SHALVDASSV
ALILRDLIAG YEGELGSSGG SNYSDYVAFL ENRQQHNDLQ YWKSLLADAE PCLLPLQQPV
HEASQAHLGH VIDQLDDITM VHQFRDRHNV SIASICQLSW ALVLANWTGS RNISFGNLSS
GRDVPIPGVQ ELVGPMINML VCNLFLDWDA STTIGGLARK LQSQSTESFE HQRASLASIQ
HELGFSKDQP LFNSTVSYRR QVSPSSDSKP AAIRLEAVIS VDPTEYDVHV NIDASSTSLE
FNLQYSTAVL SKAAATRLAE SLVQAVRIVA QNVDRPLREL TSSLLPVGDK LQLQEWNSAV
ALPVQRCVHE LVSDRIYTLP EALAISSWDG DMTYDQLGDT SRRLASYLVE QGVGPKTMVG
LCLDKSKWAI VGMLAVLFAG GAVVPLGVQH PVSRIRSIVE DTNAPIILVD DIHEKRLATM
TAHTQLLSVE SFFRASQPVS LQSGKSTVRF EDPAWIIYTS GSSGVPKGVV LQHDALATSI
LAHGPVIGVK PFDRLSQFAA YTFDVSIAEI MTALSFGACV CVPSEQDRME RLPSFLKDAN
ITIATLTSTV AALIQEDIPT IRTMVLTGEA VQSKVVDRWV QQATVINAYG PSESSIWASC
NIVKSGTDAL NIGKPLAGAF WVANPDNVGQ LVVRGAPGEL LIEGPLLARE YLNDPEKTST
AFVSDPKFMQ ELGLIPGRRL YRTGDLVQQN EDATLTYLGR IDSQIKIRGQ RVEVAEIESQ
IVRLLPGARE AVVDLVRPEG EVHDGPLILV AVIEHSDASP SLDGEVFSLD GITQIPDNAL
QALGKLDNDL GSVLPPYMVP AAFLLVSKLP INASGKLDRR VVRQKLQSTS RDTIVRFSGS
RDSIKAAPTT DLERKLQILY STALRLVPEA VGLDDSFFKL GGDSVAAMKL TAVARAQNVS
VSVADIFRWP RLADLSRVVE EKCSRDIGPL DKDPAPFSLW PELTETRTEI QTDDHNDKTR
QARLLENIAT RCNTSADQIE DVYPCSPMQA GLMAITAQRP EAYVIQRVFK LHDDLSTQQL
KDAWTRLTGK LSVLRTRIIP SASAQADALQ VVIQEAPVWQ DCRSLEVYLA TDRATPITYG
RALSRTAVVD DPAGRYFIWT VHHSIYDGWS VARTMEMLTQ LLSGQSTFSP APVSRFINYL
VQQDKDQIAN FWNGHFEGAN WVRYPAIASP QYRAKPRDTL QSQMHVNLET GGPATMSTLL
RAAWALLVAT NTGANDAAIN VVLSGRMAPI DAVMDVISPL VTTVPFYVSA SKQQSVRVFL
ETIHRRATEM IPYEHTGLQN IRALVPGLGS EFDPGHTFVV QPAGESESAD LPMFKMDVER
DATSFDAFDA YALTVECTVG GQNPGDVTIE VRYDRAVLAV DTVQHLIVQL AHIVQQLAQN
AATDKPLSEL QLLTDEDCDQ LREWNSVVPP RLERCLHDLI LETMTSYPTA PAISSWDGEM
TYSELNDASQ YLAYHLVDQG IGPEVMVGLC MDKSRWAVVA ILAILRAGGV VVPLGIQQPL
LRIEGIIQDT SCPLILVDRS QEHRLASLSD RASLFSVSSF FDAVPTPLAK SHNLSTKALP
SVKPDHSAYV IFTSGSTGVP KGIILEHGAL ATSIIAHGTE FGMDIKNCDR VLQFAAYTFD
VAIQDIIATL SFGACLCIPS EHDRMNRLVP YISEAKVTFA ILTPTVAALI QPQDVPSIRT
LILGGEALPA KVVDQWIGHA NIINGYGPSE CSIHSTCHKV QHSSEASIIG RGITAKTWVI
DSSNSDQLVP IGAIGELIIE GPLLARGYLN DPLMTANSFI TDPAFVQQLG FSPNRRMYRT
GDLVQQNMDG SLVYLGRRDT QVKIRGQRVE VGEIESHILD LLPNAREAIV DVIQAAAEDQ
DVSPMLVAVI ETDVSDTEVL ASDTQMELYS PSQITQKMRE GLDGLDTDLG LVLPAYMVPT
VYLLASKLPL NASGKLDRRA VRDQLMLLPR HVLSSFSGLA NSKQMPTTPM EQKLQTLYTS
VLALKPEAVG ISDSFFRLGG DSVAAMKLTA AAHAQGIPLT VADIFQWPRL ADLAEAMEEK
GGYGSSSAGQ KDPEPFSLWP ELQTGATEKS RLLADVAVQC GVSIDKIEDV YPCSPLQAGL
MTITAQRPEA YVVQRVFKLQ GGISTQTLKA AWTQLITDLP ILRTRIIPSV QATALQVVLR
ETPIWQAGIS LEDYLTADRA IPMIYGGALS RTAILEDGSH RHFIWTVHHS IYDGWSMAKT
MEMLEGLLSG STLSHPVSVP VSRFIGYLTQ KDKDQTAMFW QKHLEGANWT RYPELPSQQH
IINPRVTSHR RLRIPKIAGS TTFIVLRAAW ALLVASKTGA DEAVINVVLS GRAAPVKNLL
HLVAPTITTV PFHVSTSTSQ SIRDFLANIQ GQATDMIPHE HTGLQNIRRM VSSLGPDFDP
GHIFVVQPAA EMESTVTNSH LQIEREASSM DAFHAQALTI ECTVGHDTRD VEVELRFDDA
VIAADDVEQL LDQFNHLVQQ LAENADKKLP LDRLQLLSPG QMAQICRWNS SIPSRVDRCI
HELVMDQMTT RPAATAVTAC DGDLTYRQLD DLSFQLALHL IETGIGPEVI VGVCMSKSKW
AVVAMLAVLR AGGVVVPLGT RQPLGRIETI ITDTAAPLIL ADRPQEQQLK GLQAPTQLLV
VESFFEKTAK PAQNTGLQVF ARSDNAAWII YTSGSTGTPK GVVLEHGALA TSILGHGKAY
GLQSDDRILQ FAAYTFDAAI QEIMTTLAFG ACICIPSEQD RVERLTSYVA ENRITMATLT
STVAALVRPR ETPTVRTIIL VGEAVQANVV DQWLQQAKVI NGYGPSECSI ASTCREIQNS
SSALNIGTAI AGGLWVVKPS STELAAFGSP GELLIDGPLL ARGYLNDPTK TAASFITDTV
FLKDLGLSGR RLYRTGDLVQ QNRDGSLTYL GRIDTQIKIR GQRVEIGEIE SQIEKHPTVR
DAVVLYPRQG PLANRLVATV VLGETSTDSQ STAIQQISQE YQGYANTQFH ELQRILSENL
VYYMIPSVWV PLAAVPVNTS GKTDRLAISR WVQSLTEDEV STLTSEETEY IDEELTTTIE
RQLRQIWSEV LDVPLHKITY TTTKFFSLGG DSITAMQVVS ACRARGILVT VRKALDYQTI
RELATQTQTT ENSSSITKIP EGNFQLSPIQ QMYFADIAAD GIRADGEYRF NQGVTLHVTR
QIDWEDLAQA LDAIVSKHAM LRARFSHSQN GWHQWVEKHV PASYRLCKHT ASDSRSMQDI
IEHSQVSLDL EHGPVFAADL IHRPDMQKED GQDGQVLHFV AHHLVIDLMS WRILLQDLET
LLVYDQPLKM DILSFPTWLE RQRQSLSKSL DNNIDPLPVT IPKADWEYWG LVPRQERSVD
LVNIQTKCDS ATTSILLSDA ANSALKTEPV EILLAAFYHS FREHFSDRPV PPIFTEGHGR
EAMDGETNLT ETVGWFTTLS PIYIPPSENN NKAIDVLRQL KDQRRRIPSR GMPYFASRFL
TPEGRERYAD HGPAEIVFNY LGRFQQLERE DALFRIDNGD DAISVAPVGN LVNISAVLDI
SATVHEGELC INIRFSPKTK HQKTLQRWAQ SYSCAIESLV GELATVTPGA TATDFPLARL
SDNDMSLIEN QYLTTMGVSS SQVQDILPCS PIQQGILLTQ MQLPRAYLIY QTCRITSSNH
NRPVAADRLI KAWKQVVARH SVLRTILMEP LPNQEKFVQI VLADPEIDVV CVDDVLDEDA
TQWFEAQSQL DPSDRRRPPH RLTTLATTSG EIYCRFDINH ALVDASSMTL IIRDLIDAYG
NGFTTGGSNY SSYIQFLQGK HQQDDLQYWK SALHNAEPCL LSPQDPTQSD AHGKILSVSK
RIEDLTMLNI FRDTHGVSIA SVCQLAWAVV LANWTNSQNI SFGNLSSGRD APIPDVQELV
GPMINMLVCH LQVDWNANVS DVARKLQSQS AEAFEHQGVS LAAIQHELGL SRGQPLFNSI
LSYKRQVPTS SSTAEIIFEG LDSEDPTEYD INIHVVASPT DLEFDILYSN TLLSESAASR
LADCLIQAVR AISENASRQL GQLNLLPAGD ANQICKWNSD MAEPREACLH DLVIQQMAAH
PTAQAVYAHD GELTYGDLDK FSRQLACYLV NQGVGPEVMI GLCMDKSKWS VVAILGILRA
GGAVLPLGVS NPLARVEAIL KDTAAPLAIV DEAQECRLGA LEADIKLINI NSFVNAVPSA
VEGLPVSEPC TSVQPNNIAW CQFTSGSTGT PKGVILEHGA LATSIYSHSQ RFGLRPGERL
LQFAAFTFDA TIYDIMAPLS FGGCTCIPSE NDRMNRLGPF ISEANVSFAF MTPAVVSLLQ
PKDVPSIKTL IVGGEIFTSK TIDQWIQHAD VKEAYGPTEC SIFSTCNDLL DSSQVRNIGM
AVAARTWVIN PFSNGQLVPI STPGELLIEG PLLSRGYLND PEKTAGSFLV DPAFVKELGL
SPGRRFYRTG DLVQQNSDGT LQYLDRIGTQ VKINGQRLEI GEIESQILLL LPNAKYAYVC
KQGSSLIGVI EATSSSRAAV VGSHSIITPN MEQQKSFEYI EASLRERLPS YMIPSTFLVI
NSFPLTDSGK LDRRRVVNLL DAIPSDKWLE YTARSQIYYA PVGRNEEILS ELWAACINLE
KKSVSRMDNF FELGGDSITA MSLVQRLAKL GLRIHVSEIF KDPVLCAMAA RVTADIDENG
EYERFSLVSS EERAQIVKEN MSPARRQLNH FAFDATGPCD TSHLTSAIIQ LVTKIESLRT
GFAQTHKQKL LQVVYAKWEP AVRVFKTDKS PGAFYEETLE RDMFIEPNLA RPMFDVAIII
TRTTQQVRIV FRMSHALYDG ATLHQVWAAL EAIMAGQTIG NFAPVGPYFQ SLRAQTTNET
EEYWGELVQG AAISSVSAGA EPRVSRLGIF SSKPIMLPRS RQFDFTLAVA VKAAWAIVLS
HHVTSNDVVF ADVLTGRTVV HPSVADVVAC CARAVPCRVT CEPEWTAKTL LEQIKQQQVD
SMAHEGLELQ QIAQRFMGWS EEVESDAPDM RVSMVNHTKA QKQTMSLGST VYERVTVDLS
NSYASVDFAI ESVEQEDGSL SLGMAYASDR ISEQLARTLS NGFQAVLLEI IEDSGCNVSH
LDDILRDLVV KQ
