ID   WAAK_SALTY              Reviewed;         381 AA.
AC   P26470;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Lipopolysaccharide 1,2-N-acetylglucosaminetransferase;
DE            EC=2.4.1.56;
GN   Name=waaK; Synonyms=rfaK; OrderedLocusNames=STM3714;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=1657881; DOI=10.1128/jb.173.22.7151-7163.1991;
RA   Maclachlan P.R., Kadam S.K., Sanderson K.E.;
RT   "Cloning, characterization, and DNA sequence of the rfaLK region for
RT   lipopolysaccharide synthesis in Salmonella typhimurium LT2.";
RL   J. Bacteriol. 173:7151-7163(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Adds the terminal N-acetyl-D-glucosamine group on the
CC       glucose(II) group of LPS.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-N-acetyl-alpha-D-glucosamine + [lipopolysaccharide] = UDP
CC         + N-acetyl-alpha-D-glucosaminyl-[lipopolysaccharide].; EC=2.4.1.56;
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M73826; AAA27207.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22573.1; -; Genomic_DNA.
DR   PIR; C41317; C41317.
DR   RefSeq; NP_462614.1; NC_003197.2.
DR   RefSeq; WP_000591818.1; NC_003197.2.
DR   AlphaFoldDB; P26470; -.
DR   SMR; P26470; -.
DR   STRING; 99287.STM3714; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   PaxDb; P26470; -.
DR   DNASU; 1255238; -.
DR   EnsemblBacteria; AAL22573; AAL22573; STM3714.
DR   GeneID; 1255238; -.
DR   KEGG; stm:STM3714; -.
DR   PATRIC; fig|99287.12.peg.3928; -.
DR   HOGENOM; CLU_009583_38_1_6; -.
DR   OMA; VHNEMFA; -.
DR   PhylomeDB; P26470; -.
DR   BioCyc; MetaCyc:STM3714-MON; -.
DR   BioCyc; SENT99287:STM3714-MON; -.
DR   UniPathway; UPA00958; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008917; F:lipopolysaccharide N-acetylglucosaminyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Membrane;
KW   Reference proteome; Transferase.
FT   CHAIN           1..381
FT                   /note="Lipopolysaccharide 1,2-N-
FT                   acetylglucosaminetransferase"
FT                   /id="PRO_0000080306"
SQ   SEQUENCE   381 AA;  43152 MW;  F60F37FF175372C6 CRC64;
     MIKKIIFTVT PIFSIPPRGA AAVETWIYQV AKRLSIPNAI ACIKNAGYPE YNKINDNCDI
     HYIGFSKVYK RLFQKWTRLD PLPYSQRILN IRDKVTTQED SVIVIHNSMK LYRQIRERNP
     NAKLVMHMHN AFEPELPDND AKIIVPSQFL KAFYEERLPA AAVSIVPNGF CAETYKRNPQ
     DNLRQQLNIA EDATVLLYAG RISPDKGILL LLQAFKQLRT LRSNIKLVVV GDPYASRKGE
     KAEYQKKVLD AAKEIGTDCI MAGGQSPDQM HNFYHIADLV IVPSQVEEAF CMVAVEAMAA
     GKAVLASKKG GISEFVLDGI TGYHLAEPMS SDSIINDINR ALADKERHQI AEKAKSLVFS
     KYSWENVAQR FEEQMKNWFD K