ID   WAAU_ECOLI              Reviewed;         357 AA.
AC   P27242; Q2M7T7;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Lipopolysaccharide 1,2-N-acetylglucosaminetransferase;
DE            EC=2.4.1.56;
GN   Name=waaU; Synonyms=rfaK, waaK; OrderedLocusNames=b3623, JW3598;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1624462; DOI=10.1128/jb.174.14.4746-4752.1992;
RA   Klena J.D., Pradel E., Schnaitman C.A.;
RT   "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and
RT   rfaZ of Escherichia coli K-12 and Salmonella typhimurium.";
RL   J. Bacteriol. 174:4746-4752(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA   Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT   from 76.0 to 81.5 minutes.";
RL   Nucleic Acids Res. 22:2576-2586(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Adds the terminal N-acetyl-D-glucosamine group on the
CC       glucose(II) group of LPS.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-N-acetyl-alpha-D-glucosamine + [lipopolysaccharide] = UDP
CC         + N-acetyl-alpha-D-glucosaminyl-[lipopolysaccharide].; EC=2.4.1.56;
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
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DR   EMBL; M95398; AAA24523.1; -; Genomic_DNA.
DR   EMBL; U00039; AAB18600.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76647.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77669.1; -; Genomic_DNA.
DR   PIR; A65163; A65163.
DR   RefSeq; NP_418080.1; NC_000913.3.
DR   RefSeq; WP_001236433.1; NZ_LN832404.1.
DR   AlphaFoldDB; P27242; -.
DR   SMR; P27242; -.
DR   BioGRID; 4263305; 249.
DR   DIP; DIP-10672N; -.
DR   IntAct; P27242; 7.
DR   STRING; 511145.b3623; -.
DR   CAZy; GT9; Glycosyltransferase Family 9.
DR   jPOST; P27242; -.
DR   PaxDb; P27242; -.
DR   PRIDE; P27242; -.
DR   EnsemblBacteria; AAC76647; AAC76647; b3623.
DR   EnsemblBacteria; BAE77669; BAE77669; BAE77669.
DR   GeneID; 948147; -.
DR   KEGG; ecj:JW3598; -.
DR   KEGG; eco:b3623; -.
DR   PATRIC; fig|511145.12.peg.3743; -.
DR   EchoBASE; EB1393; -.
DR   eggNOG; COG0859; Bacteria.
DR   HOGENOM; CLU_056162_2_0_6; -.
DR   InParanoid; P27242; -.
DR   OMA; HIASGFN; -.
DR   PhylomeDB; P27242; -.
DR   BioCyc; EcoCyc:EG11423-MON; -.
DR   UniPathway; UPA00958; -.
DR   PRO; PR:P27242; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008713; F:ADP-heptose-lipopolysaccharide heptosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008917; F:lipopolysaccharide N-acetylglucosaminyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IMP:EcoCyc.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IBA:GO_Central.
DR   CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR   InterPro; IPR002201; Glyco_trans_9.
DR   Pfam; PF01075; Glyco_transf_9; 1.
PE   4: Predicted;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Membrane;
KW   Reference proteome; Transferase.
FT   CHAIN           1..357
FT                   /note="Lipopolysaccharide 1,2-N-
FT                   acetylglucosaminetransferase"
FT                   /id="PRO_0000097283"
FT   CONFLICT        293
FT                   /note="I -> M (in Ref. 1; AAA24523)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   357 AA;  41729 MW;  951523B8192616F5 CRC64;
     MRLGTFHKKK RFYINKIKIN FLSFLFRNKI NNQITDPAQV KSCLIIHDNN KLGDLIVLSS
     IYRELYSKGV KITLLTNRKG GEFLSNNKNI FEFCIKESTG FLEMLTLCKH LRDLQFDIVL
     DPFETMPSFK HSLILSSLKD SYILGFDHWY KRYYSFYHPH DECLKEHMST RAIEILKHIY
     GEGKFSTNYD LHLPVDVEDK IKEFIGDTRI VIINPLGAKK ICRLTFEQIK VIYQEVKTHF
     ENYRIIFTGL PQDLLTIPIL EIETLPFDEF IYTVALTKYS DFVISVDTAL VHIAAAYHKP
     TLAFYPNSRT PEYPSHLIWS PNHHKSIQIV SPTYTVKDID TETLTNSVKR LSCIDKK