ID   WAG24_TROWA             Reviewed;          24 AA.
AC   P58930;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 2.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Waglerin-4;
DE   AltName: Full=SL-Waglerin-2;
DE   Contains:
DE     RecName: Full=Waglerin-2;
DE     AltName: Full=Lethal peptide II;
DE     AltName: Full=Waglerin II;
OS   Tropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Tropidolaemus.
OX   NCBI_TaxID=8770;
RN   [1]
RP   PROTEIN SEQUENCE (WAGLERIN-4), AND SYNTHESIS OF 3-24 (WAGLERIN-2).
RC   TISSUE=Venom;
RX   PubMed=1440639; DOI=10.1016/0041-0101(92)90047-9;
RA   Schmidt J.J., Weinstein S.A., Smith L.A.;
RT   "Molecular properties and structure-function relationships of lethal
RT   peptides from venom of Wagler's pit viper, Trimeresurus wagleri.";
RL   Toxicon 30:1027-1036(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 3-24 (WAGLERIN-2), AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=2048140; DOI=10.1016/0041-0101(91)90107-3;
RA   Weinstein S.A., Schmidt J.J., Bernheimer A.W., Smith L.A.;
RT   "Characterization and amino acid sequences of two lethal peptides isolated
RT   from venom of Wagler's pit viper, Trimeresurus wagleri.";
RL   Toxicon 29:227-236(1991).
CC   -!- FUNCTION: Waglerin-2 selectively blocks the epsilon subunit of muscle
CC       nicotinic acetylcholine receptor (nAChR). Has also effects on rodent
CC       ionotropic GABA(A) receptors (GABR), since it potentiates I(GABA) in
CC       some neurons and depresses I(GABA) in others. In mice, it elicits
CC       tachypnea, ocular proptosis, rapid collapse and spasms, whereas no
CC       toxic effects on respiration and blood pressure are observed in rats
CC       (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Waglerin-4 selectively blocks the epsilon subunit of muscle
CC       nicotinic acetylcholine receptor. It elicits tachypnea, ocular
CC       proptosis, rapid collapse and spasms in mice. It causes death by
CC       respiratory failure (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- TOXIC DOSE: LD(50) is 0.51-0.583 mg/kg by intraperitoneal injection
CC       into mice. {ECO:0000269|PubMed:2048140}.
CC   -!- TOXIC DOSE: LD(50) of waglerin-2 is 0.51 mg/kg by intraperitoneal
CC       injection into mice. {ECO:0000269|PubMed:2048140}.
CC   -!- SIMILARITY: Belongs to the waglerin family. {ECO:0000305}.
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DR   PIR; A44008; A44008.
DR   AlphaFoldDB; P58930; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:InterPro.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012637; Toxin_33.
DR   Pfam; PF08121; Toxin_33; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Postsynaptic neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..24
FT                   /note="Waglerin-4"
FT                   /id="PRO_0000404086"
FT   PEPTIDE         3..24
FT                   /note="Waglerin-2"
FT                   /id="PRO_0000044550"
FT   DISULFID        11..15
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   24 AA;  2748 MW;  D9B533A33387594F CRC64;
     SLGGKPDLRP CYPPCHYIPR PKPR