WAGO1_CAEEL
ID WAGO1_CAEEL Reviewed; 945 AA.
AC Q21770;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Argonaute protein wago-1 {ECO:0000305};
DE AltName: Full=Worm-specific argonaute protein 1 {ECO:0000312|WormBase:R06C7.1};
GN Name=wago-1 {ECO:0000312|WormBase:R06C7.1};
GN ORFNames=R06C7.1 {ECO:0000312|WormBase:R06C7.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:CAA95839.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=11030340; DOI=10.1016/s1097-2765(00)00059-9;
RA Reinke V., Smith H.E., Nance J., Wang J., Van Doren C., Begley R.,
RA Jones S.J.M., Davis E.B., Scherer S., Ward S., Kim S.K.;
RT "A global profile of germline gene expression in C. elegans.";
RL Mol. Cell 6:605-616(2000).
RN [3]
RP GENE FAMILY.
RX PubMed=17110334; DOI=10.1016/j.cell.2006.09.033;
RA Yigit E., Batista P.J., Bei Y., Pang K.M., Chen C.C., Tolia N.H.,
RA Joshua-Tor L., Mitani S., Simard M.J., Mello C.C.;
RT "Analysis of the C. elegans Argonaute family reveals that distinct
RT Argonautes act sequentially during RNAi.";
RL Cell 127:747-757(2006).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19800275; DOI=10.1016/j.molcel.2009.09.020;
RA Gu W., Shirayama M., Conte D. Jr., Vasale J., Batista P.J., Claycomb J.M.,
RA Moresco J.J., Youngman E.M., Keys J., Stoltz M.J., Chen C.C., Chaves D.A.,
RA Duan S., Kasschau K.D., Fahlgren N., Yates J.R., Mitani S.,
RA Carrington J.C., Mello C.C.;
RT "Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in
RT the C. elegans germline.";
RL Mol. Cell 36:231-244(2009).
RN [5]
RP INTERACTION WITH RDE-12, AND SUBCELLULAR LOCATION.
RX PubMed=24684931; DOI=10.1016/j.cub.2014.03.008;
RA Shirayama M., Stanney W., Gu W., Seth M., Mello C.C.;
RT "The vasa homolog rde-12 engages target mRNA and multiple argonaute
RT proteins to promote RNAi in C. elegans.";
RL Curr. Biol. 24:845-851(2014).
RN [6]
RP INTERACTION WITH ZNFX-1.
RX PubMed=29775580; DOI=10.1016/j.molcel.2018.04.009;
RA Ishidate T., Ozturk A.R., Durning D.J., Sharma R., Shen E.Z., Chen H.,
RA Seth M., Shirayama M., Mello C.C.;
RT "ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.";
RL Mol. Cell 70:639-649(2018).
CC -!- FUNCTION: Argonaute protein which is involved in the endogenous small
CC interfering RNA (endo-siRNA) pathway. Interacts with secondary 22G-
CC RNAs, which are RNA-dependent RNA polymerase-derived endo-siRNAs,
CC typically 22 nucleotides in length with a 5'guanosine residue. In the
CC germline, functions in a genome surveillance system to silence
CC transposons and aberrant transcripts. {ECO:0000269|PubMed:19800275}.
CC -!- SUBUNIT: Interacts with rde-12 (PubMed:24684931). Interacts with znfx-1
CC (PubMed:29775580). {ECO:0000269|PubMed:24684931,
CC ECO:0000269|PubMed:29775580}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic granule {ECO:0000269|PubMed:19800275,
CC ECO:0000269|PubMed:24684931}.
CC -!- TISSUE SPECIFICITY: Enriched in sperm and oocytes.
CC {ECO:0000269|PubMed:11030340}.
CC -!- MISCELLANEOUS: Members of the WAGO (worm-specific argonaute) subfamily
CC lack conserved metal-binding residues found in other argonaute proteins
CC and probably do not cleave target mRNAs directly.
CC {ECO:0000303|PubMed:17110334}.
CC -!- SIMILARITY: Belongs to the Argonaute family. WAGO subfamily.
CC {ECO:0000305}.
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DR EMBL; BX284601; CAA95839.1; -; Genomic_DNA.
DR PIR; T23965; T23965.
DR RefSeq; NP_492045.1; NM_059644.5.
DR AlphaFoldDB; Q21770; -.
DR SMR; Q21770; -.
DR BioGRID; 37906; 25.
DR STRING; 6239.R06C7.1; -.
DR EPD; Q21770; -.
DR PaxDb; Q21770; -.
DR PeptideAtlas; Q21770; -.
DR EnsemblMetazoa; R06C7.1.1; R06C7.1.1; WBGene00011061.
DR GeneID; 172463; -.
DR KEGG; cel:CELE_R06C7.1; -.
DR UCSC; R06C7.1; c. elegans.
DR CTD; 172463; -.
DR WormBase; R06C7.1; CE06244; WBGene00011061; wago-1.
DR eggNOG; KOG1041; Eukaryota.
DR GeneTree; ENSGT00940000173649; -.
DR HOGENOM; CLU_310185_0_0_1; -.
DR InParanoid; Q21770; -.
DR OMA; KCCNIFF; -.
DR OrthoDB; 220258at2759; -.
DR PhylomeDB; Q21770; -.
DR Reactome; R-CEL-203927; MicroRNA (miRNA) biogenesis.
DR Reactome; R-CEL-426486; Small interfering RNA (siRNA) biogenesis.
DR Reactome; R-CEL-5578749; Transcriptional regulation by small RNAs.
DR PRO; PR:Q21770; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00011061; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0043186; C:P granule; IDA:WormBase.
DR GO; GO:0017151; F:DEAD/H-box RNA helicase binding; IPI:WormBase.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0035198; F:miRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; SSF101690; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..945
FT /note="Argonaute protein wago-1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000087436"
FT DOMAIN 321..432
FT /note="PAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00142,
FT ECO:0000305"
FT DOMAIN 636..899
FT /note="Piwi"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..29
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 945 AA; 105437 MW; 6751A5184FD54BF7 CRC64;
MSPHPPQPHP PMPPMPPVTA PPGAMTPMPP VPADAQKLHQ STGNDACIKR LQQLNVEDGA
KMYMKPTEPG KMGRPVDIQT NVFGIEVTKE TTVHRFMVHA KADLTSTKEV TFTKKGKEDF
VVQDRRDKCC NIFFLAVEKN PEFFKMKDGN QIVYDGQSTL YTTVNLFSEL DANGTKSKVF
QINGADTGND DLKTLPCISL EIYAPRDNSI TLSSENLGKR TADQNIEVNN REYTQFLELA
LNQHCVRETN RFGCFEHGKV YFLNATEEGF DQRDCVDVGD GKQLYPGLKK TIQFIEGPYG
RGQNNPSLVI DGMKAAFHKE QTVIQKLFDI TGQDPSNGLN NMTREKAAAV IKGLDCYSTY
TNRKRHLRIE GIFHESATKT RFELPDGKTC SIAEYYADKY KISLQYPNAN LVVCKDRGNN
NYFPAELMTV SRNQRVTIPQ QTGNQSQKTT KECAVLPDVR QRMIITGKNA VNITLENELL
VALGIKVYSE PLMVQARELD GKELVYQRSV MSDMGKWRAP PGWFVKPATV PDLWAAYAVG
NPGCRFSIGD VNQLVGMFID SCKKKGMVIK PPCETGLYST EKIMTQLEKV AASKCKYVLM
ITDDAIVHLH KQYKALEQRT MMIVQDMKIS KANAVVKDGK RLTLENIINK TNVKLGGLNY
TVSDAKKSMT DEQLIIGVGV SAPPAGTKYM MDNKGHLNPQ IIGFASNAVA NHEFVGDFVL
APSGQDTMAS IEDVLQNSID LFEKNRKALP KRIIIYRSGA SEGSHASILA YEIPLARAII
HGYSKEIKLI FIVVTKEHSY RFFRDQLRSG GKATEMNIPP GIVLDNAVTN PACKQFFLNG
HTTLQGTAKT PLYTVLADDC KAPMDRLEEL TFTLCHHHQI VSLSTSIPTP LYVANEYAKR
GRDLWGELTT KGPIEAKESQ GERLKELTKE IGYKQTDLNQ KRVNA
