WAKLO_ARATH
ID WAKLO_ARATH Reviewed; 708 AA.
AC Q8RY67; O80461;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Wall-associated receptor kinase-like 14;
DE EC=2.7.11.-;
DE Flags: Precursor;
GN Name=WAKL14; OrderedLocusNames=At2g23450; ORFNames=F26B6.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY ORGANIZATION.
RX PubMed=12068092; DOI=10.1104/pp.011028;
RA Verica J.A., He Z.-H.;
RT "The cell wall-associated kinase (WAK) and WAK-like kinase gene family.";
RL Plant Physiol. 129:455-459(2002).
CC -!- FUNCTION: Serine/threonine-protein kinase that may function as a
CC signaling receptor of extracellular matrix component.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Lacks the calcium-binding EGF-like domain which is a conserved
CC feature of the wall-associated receptor kinase family. {ECO:0000305}.
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DR EMBL; AC003040; AAC23760.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07456.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07457.1; -; Genomic_DNA.
DR EMBL; AY075606; AAL91622.1; -; mRNA.
DR EMBL; BT000817; AAN33192.1; -; mRNA.
DR PIR; T01134; T01134.
DR RefSeq; NP_565552.1; NM_127909.2.
DR RefSeq; NP_850041.1; NM_179710.3.
DR AlphaFoldDB; Q8RY67; -.
DR SMR; Q8RY67; -.
DR BioGRID; 2229; 8.
DR IntAct; Q8RY67; 7.
DR STRING; 3702.AT2G23450.2; -.
DR PaxDb; Q8RY67; -.
DR PRIDE; Q8RY67; -.
DR ProteomicsDB; 242744; -.
DR EnsemblPlants; AT2G23450.1; AT2G23450.1; AT2G23450.
DR EnsemblPlants; AT2G23450.2; AT2G23450.2; AT2G23450.
DR GeneID; 816877; -.
DR Gramene; AT2G23450.1; AT2G23450.1; AT2G23450.
DR Gramene; AT2G23450.2; AT2G23450.2; AT2G23450.
DR KEGG; ath:AT2G23450; -.
DR Araport; AT2G23450; -.
DR TAIR; locus:2046728; AT2G23450.
DR eggNOG; KOG1187; Eukaryota.
DR HOGENOM; CLU_000288_43_5_1; -.
DR InParanoid; Q8RY67; -.
DR OMA; SCYSEAR; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; Q8RY67; -.
DR PRO; PR:Q8RY67; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8RY67; baseline and differential.
DR Genevisible; Q8RY67; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR045274; WAK-like.
DR PANTHER; PTHR27005; PTHR27005; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycoprotein; Kinase; Membrane; Nucleotide-binding;
KW Reference proteome; Serine/threonine-protein kinase; Signal; Transferase;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..42
FT /evidence="ECO:0000255"
FT CHAIN 43..708
FT /note="Wall-associated receptor kinase-like 14"
FT /id="PRO_0000253319"
FT TOPO_DOM 43..285
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..708
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 348..629
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 636..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 686..708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 472
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 354..362
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 376
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CARBOHYD 43
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 89
FT /note="V -> A (in Ref. 3; AAL91622/AAN33192)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 708 AA; 77873 MW; FE11B114AE3E469A CRC64;
MLRSIFDFNQ RSTKMVMISH KLDLILVFII VIGGSIFRRV SANFTVPCNG RCGGLTLPYP
FGFSNGCSIR FDCSAAEKPM IGDFSVQNVT ENSIFVGLSH NCTRKIEDMN PLFGENFAPT
SENSFLMENC NRTTDGCSIK QKFLENVLKL KSCDATGNIS CFSLDSNSSS KNSAKFFSMK
TLRNSSCSLL FSSIAFESVG VNAGIALEFE RVRLGWWLKG GCESGTCAAN TDCTDVETPH
GYAGHRCSCL DGFHGDGYTN PCQRALPECR GSKLVWRHCR SNLITIVGGT VGGAFLLAAL
AFFFFCKRRR STPLRSHLSA KRLLSEAAGN SSVAFFPYKE IEKATDGFSE KQKLGIGAYG
TVYRGKLQND EWVAIKRLRH RDSESLDQVM NEIKLLSSVS HPNLVRLLGC CIEQGDPVLV
YEYMPNGTLS EHLQRDRGSG LPWTLRLTVA TQTAKAIAYL HSSMNPPIYH RDIKSTNILL
DYDFNSKVAD FGLSRLGMTE SSHISTAPQG TPGYLDPQYH QCFHLSDKSD VYSFGVVLAE
IITGLKVVDF TRPHTEINLA ALAVDKIGSG CIDEIIDPIL DLDLDAWTLS SIHTVAELAF
RCLAFHSDMR PTMTEVADEL EQIRLSGWIP SMSLDSPAGS LRSSDRGSER SVKQSSIGSR
RVVIPQKQPD CLASVEEISD SSPISVQDPW LSAQSSPSTN TLLGNIPR
