ID   WALR_STAAN              Reviewed;         233 AA.
AC   Q7A8E1;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Transcriptional regulatory protein WalR {ECO:0000305};
GN   Name=walR; Synonyms=vicR; OrderedLocusNames=SA0017;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
RN   [3]
RP   PHOSPHORYLATION AT THR-101, AND MUTAGENESIS OF THR-101.
RX   PubMed=27989665; DOI=10.1016/j.ijmm.2016.12.001;
RA   Hardt P., Engels I., Rausch M., Gajdiss M., Ulm H., Sass P., Ohlsen K.,
RA   Sahl H.G., Bierbaum G., Schneider T., Grein F.;
RT   "The cell wall precursor lipid II acts as a molecular signal for the
RT   Ser/Thr kinase PknB of Staphylococcus aureus.";
RL   Int. J. Med. Microbiol. 307:1-10(2017).
CC   -!- FUNCTION: Member of the two-component regulatory system WalK/WalR that
CC       regulates genes involved in cell wall metabolism, virulence regulation,
CC       biofilm production, oxidative stress resistance and antibiotic
CC       resistance via direct or indirect regulation of autolysins (By
CC       similarity). Functions as a transcription regulator by direct binding
CC       to promoter regions (By similarity). {ECO:0000250|UniProtKB:Q2G2U6,
CC       ECO:0000250|UniProtKB:Q9RDT5}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by WalK on Asp-53 (By similarity). Phosphorylated
CC       by PknB on Thr-101 (PubMed:27989665). {ECO:0000250|UniProtKB:Q9RDT5,
CC       ECO:0000269|PubMed:27989665}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB41234.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000018; BAB41234.1; ALT_INIT; Genomic_DNA.
DR   PIR; B89760; B89760.
DR   RefSeq; WP_000101976.1; NC_002745.2.
DR   AlphaFoldDB; Q7A8E1; -.
DR   SMR; Q7A8E1; -.
DR   EnsemblBacteria; BAB41234; BAB41234; BAB41234.
DR   GeneID; 66838357; -.
DR   KEGG; sau:SA0017; -.
DR   HOGENOM; CLU_000445_30_4_9; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..233
FT                   /note="Transcriptional regulatory protein WalR"
FT                   /id="PRO_0000353041"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        132..231
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         101
FT                   /note="Phosphothreonine; by PknB"
FT                   /evidence="ECO:0000269|PubMed:27989665"
FT   MUTAGEN         101
FT                   /note="T->A: Complete loss of phosphorylation by PknB."
FT                   /evidence="ECO:0000269|PubMed:27989665"
SQ   SEQUENCE   233 AA;  27192 MW;  BFA71D9C2464F600 CRC64;
     MARKVVVVDD EKPIADILEF NLKKEGYDVY CAYDGNDAVD LIYEEEPDIV LLDIMLPGRD
     GMEVCREVRK KYEMPIIMLT AKDSEIDKVL GLELGADDYV TKPFSTRELI ARVKANLRRH
     YSQPAQDTGN VTNEITIKDI VIYPDAYSIK KRGEDIELTH REFELFHYLS KHMGQVMTRE
     HLLQTVWGYD YFGDVRTVDV TIRRLREKIE DDPSHPEYIV TRRGVGYFLQ QHE