ID   WAPA_BACS4              Reviewed;        2335 AA.
AC   G4NYJ6;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=tRNA3(Ser)-specific nuclease WapA;
DE            EC=3.1.-.-;
DE   AltName: Full=Cell wall-associated protein;
DE   AltName: Full=RNase WapA;
DE   AltName: Full=Toxin WapA;
DE   Flags: Precursor;
GN   Name=wapA; OrderedLocusNames=GYO_4330;
OS   Bacillus spizizenii (strain DSM 15029 / JCM 12233 / NBRC 101239 / NRRL
OS   B-23049 / TU-B-10) (Bacillus subtilis subsp. spizizenii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1052585;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10;
RX   PubMed=22493193; DOI=10.1128/jb.05675-11;
RA   Earl A.M., Eppinger M., Fricke W.F., Rosovitz M.J., Rasko D.A.,
RA   Daugherty S., Losick R., Kolter R., Ravel J.;
RT   "Whole-genome sequences of Bacillus subtilis and close relatives.";
RL   J. Bacteriol. 194:2378-2379(2012).
RN   [2]
RP   FUNCTION AS A TRNA NUCLEASE, FUNCTION AS A TOXIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10;
RX   PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA   Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA   Kaplan M.D., Low D.A., Hayes C.S.;
RT   "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC   -!- FUNCTION: Toxic component of a toxin-immunity protein module, which
CC       functions as a cellular contact-dependent growth inhibition (CDI)
CC       system. A site-specific tRNA3(Ser) nuclease, the C-terminus (residues
CC       2201-2335) probably removes 2 or 4 nucleotides from the 3' end of
CC       tRNA3(Ser) but not tRNA2(Arg) or tRNA(Glu) (upon expression in E.coli),
CC       possibly endonucleolytically. The nuclease activity is neutralized by
CC       expression of the cognate immunity protein WapI from the same strain,
CC       but not its homolog from 2 other B.subtilis strains. The C-terminus
CC       cannot be expressed on its own in E.coli, however it can be cloned in
CC       the presence of its cognate immunity protein gene wapI. Cell contact is
CC       probably necessary for growth inhibition.
CC       {ECO:0000269|PubMed:23572593}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Note=Released
CC       into the medium. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RHS/WapA nuclease family. {ECO:0000305}.
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DR   EMBL; CP002905; AEP88889.1; -; Genomic_DNA.
DR   RefSeq; WP_014115727.1; NC_016047.1.
DR   AlphaFoldDB; G4NYJ6; -.
DR   SMR; G4NYJ6; -.
DR   STRING; 1052585.GYO_4330; -.
DR   EnsemblBacteria; AEP88889; AEP88889; GYO_4330.
DR   KEGG; bst:GYO_4330; -.
DR   HOGENOM; CLU_001086_0_0_9; -.
DR   OMA; GKKIWPT; -.
DR   OrthoDB; 1639867at2; -.
DR   Proteomes; UP000002651; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0004549; F:tRNA-specific ribonuclease activity; IDA:UniProtKB.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR045351; DUF6531.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR022385; Rhs_assc_core.
DR   InterPro; IPR031325; RHS_repeat.
DR   InterPro; IPR006530; YD.
DR   Pfam; PF20148; DUF6531; 1.
DR   Pfam; PF05593; RHS_repeat; 3.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
DR   TIGRFAMs; TIGR01643; YD_repeat_2x; 6.
PE   1: Evidence at protein level;
KW   Cell wall; Endonuclease; Hydrolase; Nuclease; Repeat; Secreted; Signal;
KW   Toxin; Virulence.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..2335
FT                   /note="tRNA3(Ser)-specific nuclease WapA"
FT                   /id="PRO_0000423976"
FT   REPEAT          1032..1065
FT                   /note="YD 1"
FT   REPEAT          1076..1103
FT                   /note="YD 2"
FT   REPEAT          1678..1716
FT                   /note="YD 3"
FT   REPEAT          1898..1935
FT                   /note="YD 4"
FT   REPEAT          2082..2113
FT                   /note="YD 5"
FT   REGION          36..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2251..2285
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..56
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        64..81
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2335 AA;  259015 MW;  7475F5C4CC695FA1 CRC64;
     MKKRKRRTFK RFIAAFLVLS LMISLLPADV LAKTTEEEAG NRIVSDDPEE TPRNEQTEEA
     VPFPSKDINK EGEITSERTE NTKLYYEGDG VYKQEVYLDP IHTKETPNAD WEDISPELKE
     STSKQVETEN AILNSDFQKQ MKNGLYATFE HNDHKVTYSL VEAKGPNKTS LTPKDTSADY
     KTDSNEIVYP DVFPNIDLQT FTFNENIKED LVLHQYDGYN TFTFQVKTDL QAKEQEDGSI
     DFSDEKGKVV FSVPKPFMTD SKLDELSGEV ERSDKVSYKL EKNEEGYLLH LTADENWLKD
     PERVYPVSID PSTSLSVSSD TFVMSAYPTT NYSASSQKWD ANLKSYVLKT GYYDKTTGTN
     YAFMKFNNLK PIQNMAVTKA TLKTYVAHSY YGTKATGLWL DTVNSNYDNG KVTWNTKPAS
     KNIGKAEVHK GQWASYDVTA AVKSWNSGGA NYGFKLHTNG NGKEYWKKLI SSANSANKPY
     IEVTYTIPKG NTPSIKAYHN GDSTGYFDIS WKKVEGAKGY KVWIYNGKEY QAISAGNVTS
     WSTKGKKIWP TSAEIASKRY KLHVDGKDGA ELALDPSPVY KNSGGSYATS KNYWIGVSAI
     FDQGEGAMSA PAKPVIPNVG KAQAPSTKGY NNGNATGYFD LSWKAVSGAT GYKVQVFNGK
     GFETLDLGNQ TSWTTKGKKI WPTSAEIKAG KYALHLKDGN GAELPINPGP TYKNAGGDGA
     KKNYSFKIIA YNKDGEAIAS PAANPTLPDI AKPKNLTGYL YTNTKSSQTG YVNLIWEKVQ
     NAKGYKVNIY NGKEYQSYDV GDVDHWTTQN KNIWPTPEEI KAGSYKLHTD GKGRELALDP
     SPVYNNANGN YKGKKNYSFT LSAYNANGET IPTAPFNPTF HEGAEFLGTE EYWSIIDIPS
     GQLNGATGNV IVNEEDLSID GRGPGLGLSR TYNSLDTSDH LFGQGWYADA ETSVISTDGG
     AMYIDEDATT HRFTKKADGT YQPPTGVYLE LTETADQFIL KTKDQTNAYF NKKGGKLQKV
     VDGHNNATVY TYNDKNQLTA ITDASGRKLT FTYDENGHVT SITGPKNKKV TYSYESDLLK
     KVTDTDGTVT SFDYDAEGRL VKQYSANSTE AKPVFTEYQY SGHRLEKAIN AKKETYVYSY
     DADKKTLLMT QPNGRKVQYG YNEAGNPIQV IDDAEGLKIT TNTKYEGNNV VEDVDPNDVG
     TGKATESYQY DKDGNVTSVK DAYGTETYEY NKNNDVTKMK DTEGNVTDIA YDGLDAVSET
     DQSGKSSSAA VYDKYGNQIQ SSKDLSASTN ILKDGSFEAQ KSGWNLTASK DSGKISVIAD
     KSGVLSGSKA LEVLSQSTSA GTDHGYSSAT QTVELEPNTT YTLSGKIKTD LAKSRAYFNI
     DLRDKDQKRI QWIHNEYSAL AGKNDWTKRQ ITFTTPANAG KAVVYMEVDH RDKDGKGKAW
     FGEVQLEKGE VSSSYNPVQN SSFTAATENW SVSGASVDSE EGFNDDVSLK AARTSASQAG
     SVTKQTVVLG QNANDKPVYL TLTGMSKASS VKFTDEKDYS LQANVTYADG STGVYNAKFP
     SGTQEWNRAA VVIPKTKPIN KVDISILFQK SATGTVWFDD IRLIEGSLLT KSTYDSNGNY
     VTKEEDELGF STSTDYDETG KKTAETDAKG EKTTYTYDQA DQLTNMTLSN GTSILHSYDK
     EGNEVSKTIR AGADQTYKYE YDVMGKLVKT TDPLGNVLAS EYDANSNLTK TISPNGNEVS
     LSYDGTDRVK SKSYNGTEKY NFTYDKNGNE TSVVNKEQNT TKKRTFDNKN RLTELTDRGG
     SQTWTYPSDS DKLKTFSWSH GDQKGTNQFT YNKLDQMIEM KDSTSSYSFD YDENGNVQTF
     ITGNGGGTSF SYDERNLVSS LHIGDKNGGS ILTESYEYDA NGNRTTINSS ASGKVQYEYG
     KLNQLVKETH EDGTVIEYTY DGFGNRKTVT TVKDGSSKTV NASFNIMNQL TKVNDESISY
     DKNGNRTSDG KFTYTWDAED NLTAVTKKGE DKPFATYKYD EKGNRIQKTV NGNVTNYFYD
     GDSLNVLYET DADNKVTKSY TYGDSGQLLS YTENGKKYFY HYNAHGDVIA ISDSSGKTLA
     KYQYDAWGNP TKTEASDEVK DNRYRYAGYQ YDEETGLYYL MARYYEPRNG VFLSLDPDPG
     SDGDSLDQNG YAYGNNNPVM NVDPDGHWVW LVVNAGFAAY DGYKAYKSGK GWKGAAWAAA
     SNFGPGKIFK GAKRVYRFAK SGKNFNWKHI KKDHGPKSKA RMPNGQPKSK FRSAKTLRRT
     TKATARTRPA YVQKDGRTVH FKKFKKPIGR KTNGRHTYTV KVVKSGRYVV TSYPY